BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation1683 1 stem_loop range1683 1 13 35 annotation7019 1 BBa_B0011 range7019 1 1 46 BBa_K2023009 1 BBa_K2023009 Gaussia luciferase coding sequence optimized for use in <i>E.coli</i> DH5-Alpha 2016-10-13T11:00:00Z 2016-10-20T04:19:03Z IDT synthesis, extraction from part 3 Gaussia luciferase coding sequence optimized for use in E.coli false false _2490_ 30356 30230 9 false Consideration of the use in E.coli false C??lia Chenebault annotation2518419 1 GLuc range2518419 1 1 561 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K2023002 1 BBa_K2023002 XylR coding device 2016-10-13T11:00:00Z 2016-10-20T01:45:44Z IDT synthesis XylR coding device used to create our biosensor false false _2490_ 30356 30230 9 false blabla false C??lia Chenebault component2517754 1 BBa_B0034 component2517756 1 BBa_K2023005 component2517752 1 BBa_I723018 component2517763 1 BBa_B0014 annotation2517756 1 BBa_K2023005 range2517756 1 437 2140 annotation2517754 1 BBa_B0034 range2517754 1 419 430 annotation2517752 1 BBa_I723018 range2517752 1 1 410 annotation2517763 1 BBa_B0014 range2517763 1 2149 2243 BBa_I723018 1 Pr Pr (promoter for XylR) 2007-10-24T11:00:00Z 2015-08-31T04:07:54Z [Christine/Maia] This is the naturally-occurring (possibly stationary-phase only) promoter for the XylR gene (which encodes the transcriptional regulator XylR). false false _126_ 0 2140 9 Not in stock false [Christine/Maia] false Scott Ramsay annotation1955191 1 Pr range1955191 1 1 410 BBa_I723020 1 Pu Pu 2007-10-24T11:00:00Z 2015-08-31T04:07:54Z [Christine/Maia] This is the promoter activated by the transcriptional regulator XylR (BBa_I723136) in response to detection of environmental xylene. false false _126_ 0 2140 9 It's complicated false [Christine/Maia] true Scott Ramsay annotation1955192 1 Pu range1955192 1 1 320 BBa_K2023001 1 BB123 Biosensor device for BTEX detection 2016-10-13T11:00:00Z 2016-10-20T12:05:53Z IDT synthesis in 3 parts that were then assembled The Pr promoter is a constitutive promoter driving the transcription of the XylR gene, coding for the XylR protein. The XylR protein, a transcriptional regulatory protein for the Pu promoter, is activated by aromatic hydrocarbons that carry a methyl group (like toluene and xylene). In our biosensor the Pu promoter allows the transcription of the bioluminescent reporter gene GLuc, coding for the Gaussia luciferase. When this enzyme reacts with its coelenterazine substrate it emits luminescence. false false _2490_ 30230 30230 9 false Site directed mutagenesis to overcome the mutation in the start codon of XylR false Celia Chenebault, Camille Soucies, Benjamin Piot component2518713 1 BBa_K2023002 component2518727 1 BBa_K2023003 annotation2518727 1 BBa_K2023003 range2518727 1 2252 3261 annotation2518713 1 BBa_K2023002 range2518713 1 1 2243 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939303 1 BBa_B0012 component939311 1 BBa_B0011 annotation939311 1 BBa_B0011 range939311 1 50 95 annotation939303 1 BBa_B0012 range939303 1 1 41 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K2023003 1 BBa_K2023003 Gluc coding device with Pu 2016-10-13T11:00:00Z 2016-10-20T03:23:39Z IDT synthesis Gluc coding device with Pu false false _2490_ 30356 30230 9 false blabla false C??lia Chenebault component2518688 1 BBa_K2023009 component2518686 1 BBa_B0034 component2518684 1 BBa_I723020 component2518695 1 BBa_B0014 annotation2518695 1 BBa_B0014 range2518695 1 916 1010 annotation2518686 1 BBa_B0034 range2518686 1 329 340 annotation2518688 1 BBa_K2023009 range2518688 1 347 907 annotation2518684 1 BBa_I723020 range2518684 1 1 320 BBa_K2023005 1 BBa_K2023005 XylR gene 2016-10-13T11:00:00Z 2016-10-19T07:51:02Z IDT synthesis XylR gene optimized for E.coli to synthesis our biosensor false false _2490_ 30356 30230 9 false Blabla false C??lia Chenebault annotation2517452 1 XylR range2517452 1 1 1704 BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0034_sequence 1 aaagaggagaaa BBa_I723020_sequence 1 cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgc BBa_K2023009_sequence 1 atgggagtgaaagttctttttgcccttatttgtattgctgtggccgaggccaaaccaactgaaaacaatgaagatttcaacattgtagctgtagctagcaactttgctacaacggatctcgatgctgaccgtggtaaattgcccggaaaaaaattaccacttgaggtactcaaagaaatggaagccaatgctaggaaagctggctgcactaggggatgtctgatatgcctgtcacacatcaagtgtacacccaaaatgaagaagtttatcccaggaagatgccacacctatgaaggagacaaagaaagtgcacagggaggaataggagaggctattgttgacattcctgaaattcctgggtttaaggatttggaacccatggaacaattcattgcacaagttgacctatgtgtagactgcacaactggatgcctcaaaggtcttgccaatgtgcaatgttctgatttactcaagaaatggctgccacaaagatgtgcaacttttgctagcaaaattcaaggccaagtggacaaaataaagggtgccggtggtgattaatag BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_K2023005_sequence 1 atgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggacttatgagaaccgccatggacagatgcgggcaaaatatatctcaggccgcacgtttgcttggcttgaccagacccgcaatggcgtatcgcttaaaaaaactggatcctagtttgtcagtcaaggctacggggcgttaatag BBa_K2023003_sequence 1 cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgctactagagaaagaggagaaatactagatgggagtgaaagttctttttgcccttatttgtattgctgtggccgaggccaaaccaactgaaaacaatgaagatttcaacattgtagctgtagctagcaactttgctacaacggatctcgatgctgaccgtggtaaattgcccggaaaaaaattaccacttgaggtactcaaagaaatggaagccaatgctaggaaagctggctgcactaggggatgtctgatatgcctgtcacacatcaagtgtacacccaaaatgaagaagtttatcccaggaagatgccacacctatgaaggagacaaagaaagtgcacagggaggaataggagaggctattgttgacattcctgaaattcctgggtttaaggatttggaacccatggaacaattcattgcacaagttgacctatgtgtagactgcacaactggatgcctcaaaggtcttgccaatgtgcaatgttctgatttactcaagaaatggctgccacaaagatgtgcaacttttgctagcaaaattcaaggccaagtggacaaaataaagggtgccggtggtgattaatagtactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I723018_sequence 1 attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccga BBa_K2023002_sequence 1 attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagaaagaggagaaatactagatgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggacttatgagaaccgccatggacagatgcgggcaaaatatatctcaggccgcacgtttgcttggcttgaccagacccgcaatggcgtatcgcttaaaaaaactggatcctagtttgtcagtcaaggctacggggcgttaatagtactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K2023001_sequence 1 attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagaaagaggagaaatactagatgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggacttatgagaaccgccatggacagatgcgggcaaaatatatctcaggccgcacgtttgcttggcttgaccagacccgcaatggcgtatcgcttaaaaaaactggatcctagtttgtcagtcaaggctacggggcgttaatagtactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagcccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgctactagagaaagaggagaaatactagatgggagtgaaagttctttttgcccttatttgtattgctgtggccgaggccaaaccaactgaaaacaatgaagatttcaacattgtagctgtagctagcaactttgctacaacggatctcgatgctgaccgtggtaaattgcccggaaaaaaattaccacttgaggtactcaaagaaatggaagccaatgctaggaaagctggctgcactaggggatgtctgatatgcctgtcacacatcaagtgtacacccaaaatgaagaagtttatcccaggaagatgccacacctatgaaggagacaaagaaagtgcacagggaggaataggagaggctattgttgacattcctgaaattcctgggtttaaggatttggaacccatggaacaattcattgcacaagttgacctatgtgtagactgcacaactggatgcctcaaaggtcttgccaatgtgcaatgttctgatttactcaagaaatggctgccacaaagatgtgcaacttttgctagcaaaattcaaggccaagtggacaaaataaagggtgccggtggtgattaatagtactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z