BB123
type: ComponentDefinition
persistent identity: BBa K2023001
display id: BBa_K2023001
version: 1
was generated by: iGEM to SBOL conversion
description: Biosensor device for BTEX detection
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T12:05:53Z
mutable provenance: IDT synthesis in 3 parts that were then assembled
owned by: james
owned by: myers
mutable description: The Pr promoter is a constitutive promoter driving the transcription of the XylR gene, coding for the XylR protein. The XylR protein, a transcriptional regulatory protein for the Pu promoter, is activated by aromatic hydrocarbons that carry a methyl group (like toluene and xylene). In our biosensor the Pu promoter allows the transcription of the bioluminescent reporter gene GLuc, coding for the Gaussia luciferase. When this enzyme reacts with its coelenterazine substrate it emits luminescence.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2490_
m user id: 30230
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Site directed mutagenesis to overcome the mutation in the start codon of XylR
star: false
creator: Celia Chenebault, Camille Soucies, Benjamin Piot
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_K2023003
component: BBa_K2023002
sequence annotation: BBa_K2023002
sequence annotation: BBa_K2023003
sequence: https://synbiohub.org/public/igem/BBa_K2023001_sequence/1

BBa_K2023003
type: Component
persistent identity: component2518727
display id: component2518727
version: 1
top level: BB123
definition: BBa_K2023003
access: public

BBa_K2023002
type: Component
persistent identity: component2518713
display id: component2518713
version: 1
top level: BB123
definition: BBa_K2023002
access: public

BBa_K2023002
type: SequenceAnnotation
persistent identity: annotation2518713
display id: annotation2518713
version: 1
top level: BB123
location: https://synbiohub.org/public/igem/BBa_K2023001/annotation2518713/range2518713/1
component: BBa_K2023002

https://synbiohub.org/public/igem/BBa_K2023001/annotation2518713/range2518713/1
type: Range
persistent identity: range2518713
display id: range2518713
version: 1
top level: BB123
direction: direction/reverse
start: 1
end: 2243
orientation: inline

BBa_K2023003
type: SequenceAnnotation
persistent identity: annotation2518727
display id: annotation2518727
version: 1
top level: BB123
location: https://synbiohub.org/public/igem/BBa_K2023001/annotation2518727/range2518727/1
component: BBa_K2023003

https://synbiohub.org/public/igem/BBa_K2023001/annotation2518727/range2518727/1
type: Range
persistent identity: range2518727
display id: range2518727
version: 1
top level: BB123
direction: direction/reverse
start: 2252
end: 3261
orientation: inline

BBa_K2023002
type: ComponentDefinition
persistent identity: BBa K2023002
display id: BBa_K2023002
version: 1
was generated by: iGEM to SBOL conversion
description: XylR coding device
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T01:45:44Z
mutable provenance: IDT synthesis
owned by: james
owned by: myers
mutable description: XylR coding device used to create our biosensor
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: blabla
star: false
creator: C??lia Chenebault
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_B0014
component: BBa_K2023005
component: BBa_B0034
component: BBa_I723018
sequence annotation: BBa_I723018
sequence annotation: BBa_B0034
sequence annotation: BBa_K2023005
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K2023002_sequence/1

BBa_B0014
type: Component
persistent identity: component2517763
display id: component2517763
version: 1
top level: BBa_K2023002
definition: BBa_B0014
access: public

BBa_K2023005
type: Component
persistent identity: component2517756
display id: component2517756
version: 1
top level: BBa_K2023002
definition: BBa_K2023005
access: public

BBa_B0034
type: Component
persistent identity: component2517754
display id: component2517754
version: 1
top level: BBa_K2023002
definition: BBa_B0034
access: public

BBa_I723018
type: Component
persistent identity: component2517752
display id: component2517752
version: 1
top level: BBa_K2023002
definition: Pr
access: public

BBa_I723018
type: SequenceAnnotation
persistent identity: annotation2517752
display id: annotation2517752
version: 1
top level: BBa_K2023002
location: https://synbiohub.org/public/igem/BBa_K2023002/annotation2517752/range2517752/1
component: BBa_I723018

https://synbiohub.org/public/igem/BBa_K2023002/annotation2517752/range2517752/1
type: Range
persistent identity: range2517752
display id: range2517752
version: 1
top level: BBa_K2023002
direction: direction/reverse
start: 1
end: 410
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2517754
display id: annotation2517754
version: 1
top level: BBa_K2023002
location: https://synbiohub.org/public/igem/BBa_K2023002/annotation2517754/range2517754/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023002/annotation2517754/range2517754/1
type: Range
persistent identity: range2517754
display id: range2517754
version: 1
top level: BBa_K2023002
direction: direction/reverse
start: 419
end: 430
orientation: inline

BBa_K2023005
type: SequenceAnnotation
persistent identity: annotation2517756
display id: annotation2517756
version: 1
top level: BBa_K2023002
location: https://synbiohub.org/public/igem/BBa_K2023002/annotation2517756/range2517756/1
component: BBa_K2023005

https://synbiohub.org/public/igem/BBa_K2023002/annotation2517756/range2517756/1
type: Range
persistent identity: range2517756
display id: range2517756
version: 1
top level: BBa_K2023002
direction: direction/reverse
start: 437
end: 2140
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2517763
display id: annotation2517763
version: 1
top level: BBa_K2023002
location: https://synbiohub.org/public/igem/BBa_K2023002/annotation2517763/range2517763/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2023002/annotation2517763/range2517763/1
type: Range
persistent identity: range2517763
display id: range2517763
version: 1
top level: BBa_K2023002
direction: direction/reverse
start: 2149
end: 2243
orientation: inline

BBa_K2023003
type: ComponentDefinition
persistent identity: BBa K2023003
display id: BBa_K2023003
version: 1
was generated by: iGEM to SBOL conversion
description: Gluc coding device with Pu
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T03:23:39Z
mutable provenance: IDT synthesis
owned by: james
owned by: myers
mutable description: Gluc coding device with Pu
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: blabla
star: false
creator: C??lia Chenebault
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K2023009
component: BBa_B0034
component: BBa_I723020
component: BBa_B0014
sequence annotation: BBa_I723020
sequence annotation: BBa_B0034
sequence annotation: BBa_K2023009
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K2023003_sequence/1

BBa_K2023009
type: Component
persistent identity: component2518688
display id: component2518688
version: 1
top level: BBa_K2023003
definition: BBa_K2023009
access: public

BBa_B0034
type: Component
persistent identity: component2518686
display id: component2518686
version: 1
top level: BBa_K2023003
definition: BBa_B0034
access: public

BBa_I723020
type: Component
persistent identity: component2518684
display id: component2518684
version: 1
top level: BBa_K2023003
definition: Pu
access: public

BBa_B0014
type: Component
persistent identity: component2518695
display id: component2518695
version: 1
top level: BBa_K2023003
definition: BBa_B0014
access: public

BBa_I723020
type: SequenceAnnotation
persistent identity: annotation2518684
display id: annotation2518684
version: 1
top level: BBa_K2023003
location: https://synbiohub.org/public/igem/BBa_K2023003/annotation2518684/range2518684/1
component: BBa_I723020

https://synbiohub.org/public/igem/BBa_K2023003/annotation2518684/range2518684/1
type: Range
persistent identity: range2518684
display id: range2518684
version: 1
top level: BBa_K2023003
direction: direction/reverse
start: 1
end: 320
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518686
display id: annotation2518686
version: 1
top level: BBa_K2023003
location: https://synbiohub.org/public/igem/BBa_K2023003/annotation2518686/range2518686/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023003/annotation2518686/range2518686/1
type: Range
persistent identity: range2518686
display id: range2518686
version: 1
top level: BBa_K2023003
direction: direction/reverse
start: 329
end: 340
orientation: inline

BBa_K2023009
type: SequenceAnnotation
persistent identity: annotation2518688
display id: annotation2518688
version: 1
top level: BBa_K2023003
location: https://synbiohub.org/public/igem/BBa_K2023003/annotation2518688/range2518688/1
component: BBa_K2023009

https://synbiohub.org/public/igem/BBa_K2023003/annotation2518688/range2518688/1
type: Range
persistent identity: range2518688
display id: range2518688
version: 1
top level: BBa_K2023003
direction: direction/reverse
start: 347
end: 907
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2518695
display id: annotation2518695
version: 1
top level: BBa_K2023003
location: https://synbiohub.org/public/igem/BBa_K2023003/annotation2518695/range2518695/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2023003/annotation2518695/range2518695/1
type: Range
persistent identity: range2518695
display id: range2518695
version: 1
top level: BBa_K2023003
direction: direction/reverse
start: 916
end: 1010
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

Pr
type: ComponentDefinition
persistent identity: BBa I723018
display id: BBa_I723018
version: 1
was generated by: iGEM to SBOL conversion
description: Pr (promoter for XylR)
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
mutable description: This is the naturally-occurring (possibly stationary-phase only) promoter for the XylR gene (which encodes the transcriptional regulator XylR).
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: [Christine/Maia]
star: false
creator: Scott Ramsay
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Pr
sequence: https://synbiohub.org/public/igem/BBa_I723018_sequence/1

Pr
type: SequenceAnnotation
persistent identity: annotation1955191
display id: annotation1955191
version: 1
top level: Pr
location: https://synbiohub.org/public/igem/BBa_I723018/annotation1955191/range1955191/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I723018/annotation1955191/range1955191/1
type: Range
persistent identity: range1955191
display id: range1955191
version: 1
top level: Pr
direction: direction/forward
start: 1
end: 410
orientation: inline

Pu
type: ComponentDefinition
persistent identity: BBa I723020
display id: BBa_I723020
version: 1
was generated by: iGEM to SBOL conversion
description: Pu
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
mutable description: This is the promoter activated by the transcriptional regulator XylR (BBa_I723136) in response to detection of environmental xylene.
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: [Christine/Maia]
star: true
creator: Scott Ramsay
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Pu
sequence: https://synbiohub.org/public/igem/BBa_I723020_sequence/1

Pu
type: SequenceAnnotation
persistent identity: annotation1955192
display id: annotation1955192
version: 1
top level: Pu
location: https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1
type: Range
persistent identity: range1955192
display id: range1955192
version: 1
top level: Pu
direction: direction/forward
start: 1
end: 320
orientation: inline

BBa_K2023009
type: ComponentDefinition
persistent identity: BBa K2023009
display id: BBa_K2023009
version: 1
was generated by: iGEM to SBOL conversion
description: Gaussia luciferase coding sequence optimized for use in E.coli DH5-Alpha
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T04:19:03Z
mutable provenance: IDT synthesis, extraction from part 3
owned by: james
owned by: myers
mutable description: Gaussia luciferase coding sequence optimized for use in E.coli
discontinued: false
dominant: false
experience: experience/None
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Consideration of the use in E.coli
star: false
creator: C??lia Chenebault
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GLuc
sequence: https://synbiohub.org/public/igem/BBa_K2023009_sequence/1

GLuc
type: SequenceAnnotation
persistent identity: annotation2518419
display id: annotation2518419
version: 1
top level: BBa_K2023009
location: https://synbiohub.org/public/igem/BBa_K2023009/annotation2518419/range2518419/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2023009/annotation2518419/range2518419/1
type: Range
persistent identity: range2518419
display id: range2518419
version: 1
top level: BBa_K2023009
direction: direction/forward
start: 1
end: 561
orientation: inline

BBa_K2023005
type: ComponentDefinition
persistent identity: BBa K2023005
display id: BBa_K2023005
version: 1
was generated by: iGEM to SBOL conversion
description: XylR gene
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T07:51:02Z
mutable provenance: IDT synthesis
owned by: james
owned by: myers
mutable description: XylR gene optimized for E.coli to synthesis our biosensor
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Blabla
star: false
creator: C??lia Chenebault
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: XylR
sequence: https://synbiohub.org/public/igem/BBa_K2023005_sequence/1

XylR
type: SequenceAnnotation
persistent identity: annotation2517452
display id: annotation2517452
version: 1
top level: BBa_K2023005
location: https://synbiohub.org/public/igem/BBa_K2023005/annotation2517452/range2517452/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K2023005/annotation2517452/range2517452/1
type: Range
persistent identity: range2517452
display id: range2517452
version: 1
top level: BBa_K2023005
direction: direction/forward
start: 1
end: 1704
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K2023001_sequence/1
type: Sequence
persistent identity: BBa K2023001 sequence
display id: BBa_K2023001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagaaagaggagaaatactagatgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2023002_sequence/1
type: Sequence
persistent identity: BBa K2023002 sequence
display id: BBa_K2023002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagaaagaggagaaatactagatgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2023003_sequence/1
type: Sequence
persistent identity: BBa K2023003 sequence
display id: BBa_K2023003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgctactagagaaagaggagaaatactagatgggagtgaaagttctttttgcccttatttgtattgctgtggccgaggccaaaccaactgaaaacaatgaagatttcaacattgtagctgtagctagcaactttgctacaacggatctcgatgctgaccgtggtaaattgcccggaaaaaaattaccacttgaggtactcaaagaaatggaagccaatgctaggaaagctggctgcactaggggatgtctgatatgcctgtcacacatcaagtgtacacccaaaatgaagaagtttatcccaggaagatgccacacctatgaaggagacaaagaaagtgcacagggaggaataggagaggctattgttgacattcctgaaattcctgggtttaaggatttggaacccatggaacaattcattgcacaagttgacctatgtgtagactgcacaactggatgcctcaaaggtcttgccaatgtgcaatgttctgatttactcaagaaatggctgccacaaagatgtgcaacttttgctagcaaaattcaaggccaagtggacaaaataaagggtgccggtggtgattaatagtactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I723018_sequence/1
type: Sequence
persistent identity: BBa I723018 sequence
display id: BBa_I723018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I723020_sequence/1
type: Sequence
persistent identity: BBa I723020 sequence
display id: BBa_I723020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2023009_sequence/1
type: Sequence
persistent identity: BBa K2023009 sequence
display id: BBa_K2023009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggagtgaaagttctttttgcccttatttgtattgctgtggccgaggccaaaccaactgaaaacaatgaagatttcaacattgtagctgtagctagcaactttgctacaacggatctcgatgctgaccgtggtaaattgcccggaaaaaaattaccacttgaggtactcaaagaaatggaagccaatgctaggaaagctggctgcactaggggatgtctgatatgcctgtcacacatcaagtgtacacccaaaatgaagaagtttatcccaggaagatgccacacctatgaaggagacaaagaaagtgcacagggaggaataggagaggctattgttgacattcctgaaattcctgggtttaaggatttggaacccatggaacaattcattgcacaagttgacctatgtgtagactgcacaactggatgcctcaaaggtcttgccaatgtgcaatgttctgatttactcaagaaatggctgccacaaagatgtgcaacttttgctagcaaaattcaaggccaagtggacaaaataaagggtgccggtggtgattaatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2023005_sequence/1
type: Sequence
persistent identity: BBa K2023005 sequence
display id: BBa_K2023005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggacttatgagaaccgccatggacagatgcgggcaaaatatatctcaggccgcacgtttgcttggcttgaccagacccgcaatggcgtatcgcttaaaaaaactggatcctagtttgtcagtcaaggctacggggcgttaatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z