LOCUS BBa_K2023006 1113 bp DNA linear 25-Nov-2024 DEFINITION Term-Pu-RBS-Gluc-term ACCESSION BBa_K2023006 VERSION BBa_K2023006.1 FEATURES Location/Qualifiers stem_loop 8..27 /label=T7 TE misc_feature 34..34 /label=stop polyA_site 28..41 /label=polya misc_feature 1..41 /label=BBa_B0012 terminator 1..41 /label=BBa_B0012 stem_loop 62..84 /label=stem_loop misc_feature 50..95 /label=BBa_B0011 terminator 50..95 /label=BBa_B0011 terminator 1..95 /label=BBa_B0014 promoter 104..423 /label=Pu promoter 104..423 /label=BBa_I723020 misc_feature 436..439 /label=conserved RBS 432..443 /label=BBa_B0034 CDS 450..1010 /label=GLuc CDS 450..1010 /label=BBa_K2023009 stem_loop 1026..1045 /label=T7 TE misc_feature 1052..1052 /label=stop polyA_site 1046..1059 /label=polya misc_feature 1019..1059 /label=BBa_B0012 terminator 1019..1059 /label=BBa_B0012 stem_loop 1080..1102 /label=stem_loop misc_feature 1068..1113 /label=BBa_B0011 terminator 1068..1113 /label=BBa_B0011 terminator 1019..1113 /label=BBa_B0014 ORIGIN 1 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa 61 aaagccagat tattaatccg gcttttttat tattttacta gagcccggga aagcgcgatg 121 aacctttttt atcgctgcct tgatcaaatc gacaggtggt tatgcgcgat tgatgatttg 181 ctcaaataca gccagcgtgc tgtagatttt ctctcatacc ccccctttct tttttacaaa 241 gaaaatcaat aatttagatg aaataagggg atcggtataa gcaatggcat ggcggttgct 301 agctatacga gacttaaaat aaaaatagtg gtgacccttc aatgttgtat tttctcaact 361 ctgttcagat tggttgcttt cgccatgtat atcctcaaag cgggccagcc gtagccgtta 421 cgctactaga gaaagaggag aaatactaga tgggagtgaa agttcttttt gcccttattt 481 gtattgctgt ggccgaggcc aaaccaactg aaaacaatga agatttcaac attgtagctg 541 tagctagcaa ctttgctaca acggatctcg atgctgaccg tggtaaattg cccggaaaaa 601 aattaccact tgaggtactc aaagaaatgg aagccaatgc taggaaagct ggctgcacta 661 ggggatgtct gatatgcctg tcacacatca agtgtacacc caaaatgaag aagtttatcc 721 caggaagatg ccacacctat gaaggagaca aagaaagtgc acagggagga ataggagagg 781 ctattgttga cattcctgaa attcctgggt ttaaggattt ggaacccatg gaacaattca 841 ttgcacaagt tgacctatgt gtagactgca caactggatg cctcaaaggt cttgccaatg 901 tgcaatgttc tgatttactc aagaaatggc tgccacaaag atgtgcaact tttgctagca 961 aaattcaagg ccaagtggac aaaataaagg gtgccggtgg tgattaatag tactagagtc 1021 acactggctc accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa 1081 agccagatta ttaatccggc ttttttatta ttt //