LOCUS BBa_K2023011 2301 bp DNA linear 22-Dec-2024 DEFINITION XylR-His tag coding device ACCESSION BBa_K2023011 VERSION BBa_K2023011.1 FEATURES Location/Qualifiers promoter 1..410 /label=Pr promoter 1..410 /label=BBa_I723018 misc_feature 423..426 /label=conserved RBS 419..430 /label=BBa_B0034 CDS 437..2134 /label=XylR misc_feature 2135..2144 /label=Linkage misc_feature 2144..2162 /label=His Tag CDS 2161..2164 /label=STOP XylR CDS 437..2164 /label=BBa_K2023010 stem_loop 2184..2227 /label=stem_loop misc_feature 2173..2252 /label=BBa_B0010 terminator 2173..2252 /label=BBa_B0010 stem_loop 2268..2287 /label=T7 TE misc_feature 2294..2294 /label=stop polyA_site 2288..2301 /label=polya misc_feature 2261..2301 /label=BBa_B0012 terminator 2261..2301 /label=BBa_B0012 terminator 2173..2301 /label=BBa_B0015 ORIGIN 1 attttaatgt gggctgcttg gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct 61 cgacgtgatg cgtatacggg ttacccccat tgccacgttg cgccatcctt tttgcaatca 121 gtgaccactt ttccaagcaa aaataacgcc aagcagaacg aagacgttct ttttaagaag 181 cgagaacacc agaagttcgt gctgtcgggg catgcggcga cgaattggcg gataaagggg 241 atctgcgttg aggtggattt cagttaatca attggttaat ctttcaggac cacctaagca 301 aatgctaaag tggcagatgg aatgctgagc cggcaagcac aggccttgac gttgcaaggt 361 agtcatgacc gcagtgagcc tctgatgttc cgccgggtgg atcatcccga tactagagaa 421 agaggagaaa tactagatgt cacttattta taaacctaag atgcaacacg aagacatgca 481 ggatcttatt tcccaaatcc gttttgtcgc tgccgaggga aaaatttggc tgggagaaca 541 gcgtatgttg cttatgcaac tttcgacgtt ggcatcattt cggagagaaa taatatcctt 601 gataggcata gaaagagcga agggcttttt cttacggctt gggtatcaga gtggattgat 661 ggacgcagaa ttggctcgta aactgcgccc ggccatgcgt gaagaagagg tattccttgc 721 cggtccgcaa ctgtatgcgt tgaaaggcat ggtgaaagtc agattgttga ccatggatat 781 agcaatcaga gatggcagat tcaatgtcga agcggagtgg atagactcct tcgaagtgga 841 tatttgtcgg acggagctgg ggctgatgaa tgagccagtg tgctggactg tgttaggata 901 cgcaagtgga tacggtagtg cctttatggg tcgcagaata attttccagg aaaccagttg 961 tcgtggctgc ggtgacgaca aatgccttat tgtgggtaag acggcagagg aatgggggga 1021 tgtatcctcg tttgaagctt actttaaaag cgacccgata gtggatgagc ggtacgaact 1081 ccagacacag gttgctaatc tgagaaacag acttaaacag tatgacggcc agtactacgg 1141 gataggtcat tcacctgcat ataaacgcat ctgtgagaca atagacaaag ccgctcgcgg 1201 taaggtatca gtcttactgt tgggcgagac gggcgttgga aaagaagtaa tagcacggag 1261 tgtccactta cgctccgaga gagcggagca gccgttcgtg gcggttaatt gcgctgcaat 1321 tcctccggat ttaattgagt ctgaactttt cggcgtggat aagggagctt atacgggggc 1381 ggttaatgcg cgggctggaa gattcgaacg tgcaaatggg ggcacaatct tcttggatga 1441 ggtcatagaa cttacgcccc gcgcgcaggc cactttgctg cgggttttgc aggagggaga 1501 gctggaaaga gtggggggcg atagaacacg taaagtggac gttagattaa ttaccgctac 1561 caatgaaaac ttggaggaag ctgtgaaaat gggtagattt cgggcggatc tgttcttccg 1621 gcttaatgtt tttccggttc agattccacc ccttcgcgag cgggttgagg acatacccct 1681 tttagtagag cattttctgc gtcgtcatca caaagagtat ggtaaaaaga cccttgggtt 1741 atctgacaga gccatggagg catgccttca ctaccagtgg cctggcaaca tacgtgaatt 1801 ggagaacgcg ctggagcgtg gtgtaattct gacggaatcg aatgaaagta taaatgtgga 1861 aagcttattc ccagggcttg cgatggctac tgaaggtgat agactttcta gcgagggacg 1921 gcttgaagag gaatcgggag attcatggtt cagacagata atagaccaag gcgtatctct 1981 ggaagatttg gaagcaggac ttatgagaac cgccatggac agatgcgggc aaaatatatc 2041 tcaggccgca cgtttgcttg gcttgaccag acccgcaatg gcgtatcgct taaaaaaact 2101 ggatcctagt ttgtcagtca aggctacggg gcgtgggggg gggcatcatc accatcacca 2161 ctaatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 2221 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 2281 gtgggccttt ctgcgtttat a //