BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K2023011 1 BBa_K2023011 XylR-His tag coding device 2016-10-13T11:00:00Z 2016-10-20T04:33:47Z IDT synthesis XylR-His tag coding device false false _2490_ 30356 30230 9 false blabla false C??lia Chenebault component2520056 1 BBa_I723018 component2520063 1 BBa_K2023010 component2520058 1 BBa_B0034 component2520070 1 BBa_B0015 annotation2520063 1 BBa_K2023010 range2520063 1 437 2164 annotation2520056 1 BBa_I723018 range2520056 1 1 410 annotation2520070 1 BBa_B0015 range2520070 1 2173 2301 annotation2520058 1 BBa_B0034 range2520058 1 419 430 BBa_I723018 1 Pr Pr (promoter for XylR) 2007-10-24T11:00:00Z 2015-08-31T04:07:54Z [Christine/Maia] This is the naturally-occurring (possibly stationary-phase only) promoter for the XylR gene (which encodes the transcriptional regulator XylR). false false _126_ 0 2140 9 Not in stock false [Christine/Maia] false Scott Ramsay annotation1955191 1 Pr range1955191 1 1 410 BBa_K2023010 1 BBa_K2023010 XylR gene with His tag 2016-10-13T11:00:00Z 2016-10-20T04:34:10Z IDT synthesis XylR gene with His tag false false _2490_ 30356 30230 9 false blabla false C??lia Chenebault annotation2519776 1 Linkage range2519776 1 1699 1708 annotation2519778 1 STOP XylR range2519778 1 1725 1728 annotation2519777 1 His Tag range2519777 1 1708 1726 annotation2519773 1 XylR range2519773 1 1 1698 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K2023010_sequence 1 atgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggacttatgagaaccgccatggacagatgcgggcaaaatatatctcaggccgcacgtttgcttggcttgaccagacccgcaatggcgtatcgcttaaaaaaactggatcctagtttgtcagtcaaggctacggggcgtgggggggggcatcatcaccatcaccactaa BBa_K2023011_sequence 1 attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagaaagaggagaaatactagatgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggacttatgagaaccgccatggacagatgcgggcaaaatatatctcaggccgcacgtttgcttggcttgaccagacccgcaatggcgtatcgcttaaaaaaactggatcctagtttgtcagtcaaggctacggggcgtgggggggggcatcatcaccatcaccactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I723018_sequence 1 attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccga BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z