LOCUS       BBa_K2023014            1293 bp    DNA     linear       08-Dec-2025
DEFINITION  GFP coding device with Pr
ACCESSION   BBa_K2023014
VERSION     BBa_K2023014.1  
FEATURES             Location/Qualifiers
     promoter        1..410
                     /label=Pr
     promoter        1..410
                     /label=BBa_I723018
     misc_feature    423..426
                     /label=conserved
     RBS             419..430
                     /label=BBa_B0034
     CDS             437..1156
                     /label=GFP protein
     CDS             437..1156
                     /label=BBa_E0040
     stem_loop       1176..1219
                     /label=stem_loop
     misc_feature    1165..1244
                     /label=BBa_B0010
     terminator      1165..1244
                     /label=BBa_B0010
     stem_loop       1260..1279
                     /label=T7 TE
     misc_feature    1286..1286
                     /label=stop
     polyA_site      1280..1293
                     /label=polya
     misc_feature    1253..1293
                     /label=BBa_B0012
     terminator      1253..1293
                     /label=BBa_B0012
ORIGIN
        1 attttaatgt gggctgcttg gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct
       61 cgacgtgatg cgtatacggg ttacccccat tgccacgttg cgccatcctt tttgcaatca
      121 gtgaccactt ttccaagcaa aaataacgcc aagcagaacg aagacgttct ttttaagaag
      181 cgagaacacc agaagttcgt gctgtcgggg catgcggcga cgaattggcg gataaagggg
      241 atctgcgttg aggtggattt cagttaatca attggttaat ctttcaggac cacctaagca
      301 aatgctaaag tggcagatgg aatgctgagc cggcaagcac aggccttgac gttgcaaggt
      361 agtcatgacc gcagtgagcc tctgatgttc cgccgggtgg atcatcccga tactagagaa
      421 agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
      481 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      541 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      601 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
      661 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      721 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
      781 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
      841 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
      901 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
      961 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     1021 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     1081 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     1141 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1201 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1261 gctcaccttc gggtgggcct ttctgcgttt ata
//