LOCUS BBa_K2023015 3454 bp DNA linear 25-Nov-2024 DEFINITION XylR coding device-GFP coding device with Pu ACCESSION BBa_K2023015 VERSION BBa_K2023015.1 FEATURES Location/Qualifiers promoter 1..410 /label=Pr promoter 1..410 /label=BBa_I723018 misc_feature 423..426 /label=conserved RBS 419..430 /label=BBa_B0034 CDS 437..2140 /label=XylR CDS 437..2140 /label=BBa_K2023005 stem_loop 2156..2175 /label=T7 TE misc_feature 2182..2182 /label=stop polyA_site 2176..2189 /label=polya misc_feature 2149..2189 /label=BBa_B0012 terminator 2149..2189 /label=BBa_B0012 stem_loop 2210..2232 /label=stem_loop misc_feature 2198..2243 /label=BBa_B0011 terminator 2198..2243 /label=BBa_B0011 terminator 2149..2243 /label=BBa_B0014 promoter 1..2243 /label=BBa_K2023002 promoter 2252..2571 /label=Pu promoter 2252..2571 /label=BBa_I723020 misc_feature 2584..2587 /label=conserved RBS 2580..2591 /label=BBa_B0034 CDS 2598..3317 /label=GFP protein CDS 2598..3317 /label=BBa_E0040 stem_loop 3337..3380 /label=stem_loop misc_feature 3326..3405 /label=BBa_B0010 terminator 3326..3405 /label=BBa_B0010 stem_loop 3421..3440 /label=T7 TE misc_feature 3447..3447 /label=stop polyA_site 3441..3454 /label=polya misc_feature 3414..3454 /label=BBa_B0012 terminator 3414..3454 /label=BBa_B0012 terminator 3326..3454 /label=BBa_B0015 ORIGIN 1 attttaatgt gggctgcttg gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct 61 cgacgtgatg cgtatacggg ttacccccat tgccacgttg cgccatcctt tttgcaatca 121 gtgaccactt ttccaagcaa aaataacgcc aagcagaacg aagacgttct ttttaagaag 181 cgagaacacc agaagttcgt gctgtcgggg catgcggcga cgaattggcg gataaagggg 241 atctgcgttg aggtggattt cagttaatca attggttaat ctttcaggac cacctaagca 301 aatgctaaag tggcagatgg aatgctgagc cggcaagcac aggccttgac gttgcaaggt 361 agtcatgacc gcagtgagcc tctgatgttc cgccgggtgg atcatcccga tactagagaa 421 agaggagaaa tactagatgt cacttattta taaacctaag atgcaacacg aagacatgca 481 ggatcttatt tcccaaatcc gttttgtcgc tgccgaggga aaaatttggc tgggagaaca 541 gcgtatgttg cttatgcaac tttcgacgtt ggcatcattt cggagagaaa taatatcctt 601 gataggcata gaaagagcga agggcttttt cttacggctt gggtatcaga gtggattgat 661 ggacgcagaa ttggctcgta aactgcgccc ggccatgcgt gaagaagagg tattccttgc 721 cggtccgcaa ctgtatgcgt tgaaaggcat ggtgaaagtc agattgttga ccatggatat 781 agcaatcaga gatggcagat tcaatgtcga agcggagtgg atagactcct tcgaagtgga 841 tatttgtcgg acggagctgg ggctgatgaa tgagccagtg tgctggactg tgttaggata 901 cgcaagtgga tacggtagtg cctttatggg tcgcagaata attttccagg aaaccagttg 961 tcgtggctgc ggtgacgaca aatgccttat tgtgggtaag acggcagagg aatgggggga 1021 tgtatcctcg tttgaagctt actttaaaag cgacccgata gtggatgagc ggtacgaact 1081 ccagacacag gttgctaatc tgagaaacag acttaaacag tatgacggcc agtactacgg 1141 gataggtcat tcacctgcat ataaacgcat ctgtgagaca atagacaaag ccgctcgcgg 1201 taaggtatca gtcttactgt tgggcgagac gggcgttgga aaagaagtaa tagcacggag 1261 tgtccactta cgctccgaga gagcggagca gccgttcgtg gcggttaatt gcgctgcaat 1321 tcctccggat ttaattgagt ctgaactttt cggcgtggat aagggagctt atacgggggc 1381 ggttaatgcg cgggctggaa gattcgaacg tgcaaatggg ggcacaatct tcttggatga 1441 ggtcatagaa cttacgcccc gcgcgcaggc cactttgctg cgggttttgc aggagggaga 1501 gctggaaaga gtggggggcg atagaacacg taaagtggac gttagattaa ttaccgctac 1561 caatgaaaac ttggaggaag ctgtgaaaat gggtagattt cgggcggatc tgttcttccg 1621 gcttaatgtt tttccggttc agattccacc ccttcgcgag cgggttgagg acatacccct 1681 tttagtagag cattttctgc gtcgtcatca caaagagtat ggtaaaaaga cccttgggtt 1741 atctgacaga gccatggagg catgccttca ctaccagtgg cctggcaaca tacgtgaatt 1801 ggagaacgcg ctggagcgtg gtgtaattct gacggaatcg aatgaaagta taaatgtgga 1861 aagcttattc ccagggcttg cgatggctac tgaaggtgat agactttcta gcgagggacg 1921 gcttgaagag gaatcgggag attcatggtt cagacagata atagaccaag gcgtatctct 1981 ggaagatttg gaagcaggac ttatgagaac cgccatggac agatgcgggc aaaatatatc 2041 tcaggccgca cgtttgcttg gcttgaccag acccgcaatg gcgtatcgct taaaaaaact 2101 ggatcctagt ttgtcagtca aggctacggg gcgttaatag tactagagtc acactggctc 2161 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta 2221 ttaatccggc ttttttatta ttttactaga gcccgggaaa gcgcgatgaa ccttttttat 2281 cgctgccttg atcaaatcga caggtggtta tgcgcgattg atgatttgct caaatacagc 2341 cagcgtgctg tagattttct ctcatacccc ccctttcttt tttacaaaga aaatcaataa 2401 tttagatgaa ataaggggat cggtataagc aatggcatgg cggttgctag ctatacgaga 2461 cttaaaataa aaatagtggt gacccttcaa tgttgtattt tctcaactct gttcagattg 2521 gttgctttcg ccatgtatat cctcaaagcg ggccagccgt agccgttacg ctactagaga 2581 aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc 2641 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag 2701 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg 2761 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc 2821 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg 2881 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg 2941 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa 3001 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag 3061 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa 3121 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt 3181 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga 3241 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg 3301 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 3361 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 3421 ggctcacctt cgggtgggcc tttctgcgtt tata //