BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939311 1 BBa_B0011 component939303 1 BBa_B0012 annotation939311 1 BBa_B0011 range939311 1 50 95 annotation939303 1 BBa_B0012 range939303 1 1 41 BBa_K2023015 1 BBa_K2023015 XylR coding device-GFP coding device with Pu 2016-10-13T11:00:00Z 2016-10-20T03:40:10Z XylR coding device (BBa_K2023002) and GFP coding device with Pu (BBa_K2023016) XylR coding device-GFP coding device with Pu false false _2490_ 30356 30230 9 false blabla false C??lia Chenebault component2518979 1 BBa_K2023002 component2518981 1 BBa_I723020 component2518983 1 BBa_B0034 component2518985 1 BBa_E0040 component2518992 1 BBa_B0015 annotation2518992 1 BBa_B0015 range2518992 1 3326 3454 annotation2518981 1 BBa_I723020 range2518981 1 2252 2571 annotation2518985 1 BBa_E0040 range2518985 1 2598 3317 annotation2518983 1 BBa_B0034 range2518983 1 2580 2591 annotation2518979 1 BBa_K2023002 range2518979 1 1 2243 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K2023002 1 BBa_K2023002 XylR coding device 2016-10-13T11:00:00Z 2016-10-20T01:45:44Z IDT synthesis XylR coding device used to create our biosensor false false _2490_ 30356 30230 9 false blabla false C??lia Chenebault component2517756 1 BBa_K2023005 component2517754 1 BBa_B0034 component2517763 1 BBa_B0014 component2517752 1 BBa_I723018 annotation2517763 1 BBa_B0014 range2517763 1 2149 2243 annotation2517752 1 BBa_I723018 range2517752 1 1 410 annotation2517754 1 BBa_B0034 range2517754 1 419 430 annotation2517756 1 BBa_K2023005 range2517756 1 437 2140 BBa_K2023005 1 BBa_K2023005 XylR gene 2016-10-13T11:00:00Z 2016-10-19T07:51:02Z IDT synthesis XylR gene optimized for E.coli to synthesis our biosensor false false _2490_ 30356 30230 9 false Blabla false C??lia Chenebault annotation2517452 1 XylR range2517452 1 1 1704 BBa_I723018 1 Pr Pr (promoter for XylR) 2007-10-24T11:00:00Z 2015-08-31T04:07:54Z [Christine/Maia] This is the naturally-occurring (possibly stationary-phase only) promoter for the XylR gene (which encodes the transcriptional regulator XylR). false false _126_ 0 2140 9 Not in stock false [Christine/Maia] false Scott Ramsay annotation1955191 1 Pr range1955191 1 1 410 BBa_I723020 1 Pu Pu 2007-10-24T11:00:00Z 2015-08-31T04:07:54Z [Christine/Maia] This is the promoter activated by the transcriptional regulator XylR (BBa_I723136) in response to detection of environmental xylene. false false _126_ 0 2140 9 It's complicated false [Christine/Maia] true Scott Ramsay annotation1955192 1 Pu range1955192 1 1 320 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0034_sequence 1 aaagaggagaaa BBa_I723020_sequence 1 cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgc BBa_K2023015_sequence 1 attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagaaagaggagaaatactagatgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggacttatgagaaccgccatggacagatgcgggcaaaatatatctcaggccgcacgtttgcttggcttgaccagacccgcaatggcgtatcgcttaaaaaaactggatcctagtttgtcagtcaaggctacggggcgttaatagtactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagcccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_K2023005_sequence 1 atgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggacttatgagaaccgccatggacagatgcgggcaaaatatatctcaggccgcacgtttgcttggcttgaccagacccgcaatggcgtatcgcttaaaaaaactggatcctagtttgtcagtcaaggctacggggcgttaatag BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I723018_sequence 1 attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccga BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2023002_sequence 1 attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagaaagaggagaaatactagatgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggacttatgagaaccgccatggacagatgcgggcaaaatatatctcaggccgcacgtttgcttggcttgaccagacccgcaatggcgtatcgcttaaaaaaactggatcctagtttgtcagtcaaggctacggggcgttaatagtactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z