BBa_K2023015
type: ComponentDefinition
persistent identity: BBa K2023015
display id: BBa_K2023015
version: 1
was generated by: iGEM to SBOL conversion
description: XylR coding device-GFP coding device with Pu
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T03:40:10Z
mutable provenance: XylR coding device (BBa_K2023002) and GFP coding device with Pu (BBa_K2023016)
owned by: james
owned by: myers
mutable description: XylR coding device-GFP coding device with Pu
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: blabla
star: false
creator: C??lia Chenebault
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_K2023002
component: BBa_B0034
component: BBa_I723020
component: BBa_B0015
component: BBa_E0040
sequence annotation: BBa_K2023002
sequence annotation: BBa_I723020
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K2023015_sequence/1

BBa_K2023002
type: Component
persistent identity: component2518979
display id: component2518979
version: 1
top level: BBa_K2023015
definition: BBa_K2023002
access: public

BBa_B0034
type: Component
persistent identity: component2518983
display id: component2518983
version: 1
top level: BBa_K2023015
definition: BBa_B0034
access: public

BBa_I723020
type: Component
persistent identity: component2518981
display id: component2518981
version: 1
top level: BBa_K2023015
definition: Pu
access: public

BBa_B0015
type: Component
persistent identity: component2518992
display id: component2518992
version: 1
top level: BBa_K2023015
definition: BBa_B0015
access: public

BBa_E0040
type: Component
persistent identity: component2518985
display id: component2518985
version: 1
top level: BBa_K2023015
definition: GFP
access: public

BBa_K2023002
type: SequenceAnnotation
persistent identity: annotation2518979
display id: annotation2518979
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518979/range2518979/1
component: BBa_K2023002

https://synbiohub.org/public/igem/BBa_K2023015/annotation2518979/range2518979/1
type: Range
persistent identity: range2518979
display id: range2518979
version: 1
top level: BBa_K2023015
direction: direction/reverse
start: 1
end: 2243
orientation: inline

BBa_I723020
type: SequenceAnnotation
persistent identity: annotation2518981
display id: annotation2518981
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518981/range2518981/1
component: BBa_I723020

https://synbiohub.org/public/igem/BBa_K2023015/annotation2518981/range2518981/1
type: Range
persistent identity: range2518981
display id: range2518981
version: 1
top level: BBa_K2023015
direction: direction/reverse
start: 2252
end: 2571
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518983
display id: annotation2518983
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518983/range2518983/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023015/annotation2518983/range2518983/1
type: Range
persistent identity: range2518983
display id: range2518983
version: 1
top level: BBa_K2023015
direction: direction/reverse
start: 2580
end: 2591
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2518985
display id: annotation2518985
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518985/range2518985/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K2023015/annotation2518985/range2518985/1
type: Range
persistent identity: range2518985
display id: range2518985
version: 1
top level: BBa_K2023015
direction: direction/reverse
start: 2598
end: 3317
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2518992
display id: annotation2518992
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518992/range2518992/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2023015/annotation2518992/range2518992/1
type: Range
persistent identity: range2518992
display id: range2518992
version: 1
top level: BBa_K2023015
direction: direction/reverse
start: 3326
end: 3454
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

Pu
type: ComponentDefinition
persistent identity: BBa I723020
display id: BBa_I723020
version: 1
was generated by: iGEM to SBOL conversion
description: Pu
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
mutable description: This is the promoter activated by the transcriptional regulator XylR (BBa_I723136) in response to detection of environmental xylene.
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: [Christine/Maia]
star: true
creator: Scott Ramsay
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Pu
sequence: https://synbiohub.org/public/igem/BBa_I723020_sequence/1

Pu
type: SequenceAnnotation
persistent identity: annotation1955192
display id: annotation1955192
version: 1
top level: Pu
location: https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1
type: Range
persistent identity: range1955192
display id: range1955192
version: 1
top level: Pu
direction: direction/forward
start: 1
end: 320
orientation: inline

BBa_K2023002
type: ComponentDefinition
persistent identity: BBa K2023002
display id: BBa_K2023002
version: 1
was generated by: iGEM to SBOL conversion
description: XylR coding device
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T01:45:44Z
mutable provenance: IDT synthesis
owned by: james
owned by: myers
mutable description: XylR coding device used to create our biosensor
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: blabla
star: false
creator: C??lia Chenebault
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0014
component: BBa_K2023005
component: BBa_B0034
component: BBa_I723018
sequence annotation: BBa_I723018
sequence annotation: BBa_B0034
sequence annotation: BBa_K2023005
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K2023002_sequence/1

BBa_B0014
type: Component
persistent identity: component2517763
display id: component2517763
version: 1
top level: BBa_K2023002
definition: BBa_B0014
access: public

BBa_K2023005
type: Component
persistent identity: component2517756
display id: component2517756
version: 1
top level: BBa_K2023002
definition: BBa_K2023005
access: public

BBa_B0034
type: Component
persistent identity: component2517754
display id: component2517754
version: 1
top level: BBa_K2023002
definition: BBa_B0034
access: public

BBa_I723018
type: Component
persistent identity: component2517752
display id: component2517752
version: 1
top level: BBa_K2023002
definition: Pr
access: public

BBa_I723018
type: SequenceAnnotation
persistent identity: annotation2517752
display id: annotation2517752
version: 1
top level: BBa_K2023002
location: https://synbiohub.org/public/igem/BBa_K2023002/annotation2517752/range2517752/1
component: BBa_I723018

https://synbiohub.org/public/igem/BBa_K2023002/annotation2517752/range2517752/1
type: Range
persistent identity: range2517752
display id: range2517752
version: 1
top level: BBa_K2023002
direction: direction/reverse
start: 1
end: 410
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2517754
display id: annotation2517754
version: 1
top level: BBa_K2023002
location: https://synbiohub.org/public/igem/BBa_K2023002/annotation2517754/range2517754/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023002/annotation2517754/range2517754/1
type: Range
persistent identity: range2517754
display id: range2517754
version: 1
top level: BBa_K2023002
direction: direction/reverse
start: 419
end: 430
orientation: inline

BBa_K2023005
type: SequenceAnnotation
persistent identity: annotation2517756
display id: annotation2517756
version: 1
top level: BBa_K2023002
location: https://synbiohub.org/public/igem/BBa_K2023002/annotation2517756/range2517756/1
component: BBa_K2023005

https://synbiohub.org/public/igem/BBa_K2023002/annotation2517756/range2517756/1
type: Range
persistent identity: range2517756
display id: range2517756
version: 1
top level: BBa_K2023002
direction: direction/reverse
start: 437
end: 2140
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2517763
display id: annotation2517763
version: 1
top level: BBa_K2023002
location: https://synbiohub.org/public/igem/BBa_K2023002/annotation2517763/range2517763/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2023002/annotation2517763/range2517763/1
type: Range
persistent identity: range2517763
display id: range2517763
version: 1
top level: BBa_K2023002
direction: direction/reverse
start: 2149
end: 2243
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

Pr
type: ComponentDefinition
persistent identity: BBa I723018
display id: BBa_I723018
version: 1
was generated by: iGEM to SBOL conversion
description: Pr (promoter for XylR)
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
mutable description: This is the naturally-occurring (possibly stationary-phase only) promoter for the XylR gene (which encodes the transcriptional regulator XylR).
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: [Christine/Maia]
star: false
creator: Scott Ramsay
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Pr
sequence: https://synbiohub.org/public/igem/BBa_I723018_sequence/1

Pr
type: SequenceAnnotation
persistent identity: annotation1955191
display id: annotation1955191
version: 1
top level: Pr
location: https://synbiohub.org/public/igem/BBa_I723018/annotation1955191/range1955191/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I723018/annotation1955191/range1955191/1
type: Range
persistent identity: range1955191
display id: range1955191
version: 1
top level: Pr
direction: direction/forward
start: 1
end: 410
orientation: inline

BBa_K2023005
type: ComponentDefinition
persistent identity: BBa K2023005
display id: BBa_K2023005
version: 1
was generated by: iGEM to SBOL conversion
description: XylR gene
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T07:51:02Z
mutable provenance: IDT synthesis
owned by: james
owned by: myers
mutable description: XylR gene optimized for E.coli to synthesis our biosensor
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Blabla
star: false
creator: C??lia Chenebault
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: XylR
sequence: https://synbiohub.org/public/igem/BBa_K2023005_sequence/1

XylR
type: SequenceAnnotation
persistent identity: annotation2517452
display id: annotation2517452
version: 1
top level: BBa_K2023005
location: https://synbiohub.org/public/igem/BBa_K2023005/annotation2517452/range2517452/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K2023005/annotation2517452/range2517452/1
type: Range
persistent identity: range2517452
display id: range2517452
version: 1
top level: BBa_K2023005
direction: direction/forward
start: 1
end: 1704
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

https://synbiohub.org/public/igem/BBa_K2023015_sequence/1
type: Sequence
persistent identity: BBa K2023015 sequence
display id: BBa_K2023015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagaaagaggagaaatactagatgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I723020_sequence/1
type: Sequence
persistent identity: BBa I723020 sequence
display id: BBa_I723020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2023002_sequence/1
type: Sequence
persistent identity: BBa K2023002 sequence
display id: BBa_K2023002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgatactagagaaagaggagaaatactagatgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I723018_sequence/1
type: Sequence
persistent identity: BBa I723018 sequence
display id: BBa_I723018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2023005_sequence/1
type: Sequence
persistent identity: BBa K2023005 sequence
display id: BBa_K2023005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcacttatttataaacctaagatgcaacacgaagacatgcaggatcttatttcccaaatccgttttgtcgctgccgagggaaaaatttggctgggagaacagcgtatgttgcttatgcaactttcgacgttggcatcatttcggagagaaataatatccttgataggcatagaaagagcgaagggctttttcttacggcttgggtatcagagtggattgatggacgcagaattggctcgtaaactgcgcccggccatgcgtgaagaagaggtattccttgccggtccgcaactgtatgcgttgaaaggcatggtgaaagtcagattgttgaccatggatatagcaatcagagatggcagattcaatgtcgaagcggagtggatagactccttcgaagtggatatttgtcggacggagctggggctgatgaatgagccagtgtgctggactgtgttaggatacgcaagtggatacggtagtgcctttatgggtcgcagaataattttccaggaaaccagttgtcgtggctgcggtgacgacaaatgccttattgtgggtaagacggcagaggaatggggggatgtatcctcgtttgaagcttactttaaaagcgacccgatagtggatgagcggtacgaactccagacacaggttgctaatctgagaaacagacttaaacagtatgacggccagtactacgggataggtcattcacctgcatataaacgcatctgtgagacaatagacaaagccgctcgcggtaaggtatcagtcttactgttgggcgagacgggcgttggaaaagaagtaatagcacggagtgtccacttacgctccgagagagcggagcagccgttcgtggcggttaattgcgctgcaattcctccggatttaattgagtctgaacttttcggcgtggataagggagcttatacgggggcggttaatgcgcgggctggaagattcgaacgtgcaaatgggggcacaatcttcttggatgaggtcatagaacttacgccccgcgcgcaggccactttgctgcgggttttgcaggagggagagctggaaagagtggggggcgatagaacacgtaaagtggacgttagattaattaccgctaccaatgaaaacttggaggaagctgtgaaaatgggtagatttcgggcggatctgttcttccggcttaatgtttttccggttcagattccaccccttcgcgagcgggttgaggacataccccttttagtagagcattttctgcgtcgtcatcacaaagagtatggtaaaaagacccttgggttatctgacagagccatggaggcatgccttcactaccagtggcctggcaacatacgtgaattggagaacgcgctggagcgtggtgtaattctgacggaatcgaatgaaagtataaatgtggaaagcttattcccagggcttgcgatggctactgaaggtgatagactttctagcgagggacggcttgaagaggaatcgggagattcatggttcagacagataatagaccaaggcgtatctctggaagatttggaagcaggacttatgagaaccgccatggacagatgcgggcaaaatatatctcaggccgcacgtttgcttggcttgaccagacccgcaatggcgtatcgcttaaaaaaactggatcctagtttgtcagtcaaggctacggggcgttaatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z