LOCUS       BBa_K2023016            1203 bp    DNA     linear       25-Nov-2024
DEFINITION  GFP coding device with Pu
ACCESSION   BBa_K2023016
VERSION     BBa_K2023016.1  
FEATURES             Location/Qualifiers
     promoter        1..320
                     /label=Pu
     promoter        1..320
                     /label=BBa_I723020
     misc_feature    333..336
                     /label=conserved
     RBS             329..340
                     /label=BBa_B0034
     CDS             347..1066
                     /label=GFP protein
     CDS             347..1066
                     /label=BBa_E0040
     stem_loop       1086..1129
                     /label=stem_loop
     misc_feature    1075..1154
                     /label=BBa_B0010
     terminator      1075..1154
                     /label=BBa_B0010
     stem_loop       1170..1189
                     /label=T7 TE
     misc_feature    1196..1196
                     /label=stop
     polyA_site      1190..1203
                     /label=polya
     misc_feature    1163..1203
                     /label=BBa_B0012
     terminator      1163..1203
                     /label=BBa_B0012
     terminator      1075..1203
                     /label=BBa_B0015
ORIGIN
        1 cccgggaaag cgcgatgaac cttttttatc gctgccttga tcaaatcgac aggtggttat
       61 gcgcgattga tgatttgctc aaatacagcc agcgtgctgt agattttctc tcataccccc
      121 cctttctttt ttacaaagaa aatcaataat ttagatgaaa taaggggatc ggtataagca
      181 atggcatggc ggttgctagc tatacgagac ttaaaataaa aatagtggtg acccttcaat
      241 gttgtatttt ctcaactctg ttcagattgg ttgctttcgc catgtatatc ctcaaagcgg
      301 gccagccgta gccgttacgc tactagagaa agaggagaaa tactagatgc gtaaaggaga
      361 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      421 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      481 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      541 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      601 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      661 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      721 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      781 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      841 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      901 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      961 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
     1021 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagccaggc
     1081 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1141 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1201 ata
//