BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K2027051 1 BBa_K2027051 rAIP Coding Sequence with tag 2016-10-23T11:00:00Z 2016-10-24T12:32:19Z See BBa_K2027000 Coding sequence for BBa_K2027000 false false _2494_ 29123 29123 9 false See BBa_K2027000 false Michael Becich BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_Z0251 1 BBa_Z0251 T7 strong promoter 2005-04-04T11:00:00Z 2015-05-08T01:14:55Z bacteriophage T7 Promoter for T7 RNA polymerase false false _10_ 0 250 10 Not in stock false false T7.2 annotation1476333 1 consensus sequence range1476333 1 6 28 BBa_K2027000 1 rAIP Recombinant Apoptosis-Inducing Protein (L-lysine Alpha Oxidase) 2016-09-06T11:00:00Z 2016-10-25T09:20:16Z To be added before 2016 deadline. To be added before 2016 deadline. false false _2494_ 29123 29123 9 true To be added before 2016 deadline. false Charles Gleason, Michael Becich component2528824 1 BBa_B0034 component2528822 1 BBa_Z0251 component2528826 1 BBa_B0010 component2528825 1 BBa_K2027051 component2528828 1 BBa_B0012 annotation2528824 1 BBa_B0034 range2528824 1 44 55 annotation2528825 1 BBa_K2027051 range2528825 1 62 1549 annotation2528828 1 BBa_B0012 range2528828 1 1646 1686 annotation2528826 1 BBa_B0010 range2528826 1 1558 1637 annotation2528822 1 BBa_Z0251 range2528822 1 1 35 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_Z0251_sequence 1 gaaattaatacgactcactatagggagaccacaac BBa_K2027000_sequence 1 gaaattaatacgactcactatagggagaccacaactactagagaaagaggagaaatactagatggagcacttggctgattgcttggaagataaggattacgatacacttttgcagacattggacaacggtttgcctcacattaacacttctcatcacgttgtcattgttggtgccggaatggcaggtttgaccgctgccaaattgcttcaagatgcaggacacactgttacaatcttggaggctaacgacagagttggtggaagagtcgaaacttacagaaatgaaaaggagggttggtatgctgaaatgggagccatgagaatcccatcttctcatagaatcgttcagtggtttgtcaagaaattgggtgttgaaatgaacgagtttgtcatgacagatgacaacaccttctacttggttaatggagtcagagaaagaacttatgttgtccaagagaaccctgatgttttgaagtacaatgtctctgaatccgagaaaggtatttctgctgatgacttgcttgacagagccttgcagaaggttaaagaagaggtcgaagctaatggttgtaaggcagctttggagaagtacgatagatactctgttaaggaatatttgaaagaagagggtggactttccccaggtgctgttagaatgattggagatttgcttaacgagcaatctttgatgtacactgccctttccgaaatgatctatgatcaggcagacgttaatgattcagtcagttaccacgaggttacaggtggatccgatttgcttccagaagctttcttgtcagttcttgacgtccctatcttgcttaactccaaggttaagcatatcagacaatcagataagggtgttatcgtcagttatcagactggaaatgaatcaagtttgatggacctttctgctgatattgttttggtcactacaaccgccaaagccgcattgtttattgatttcgacccacctttgtctatctccaagatggaggctttgagatctgttcactacgattcttccactaagatcttgcttacttttagagacaagttctgggaagatgacggtattagaggtggaaagtcaatcacagatggaccaagtagatacatctactatccttcacatagttttcacaccaacgagactatcggtgttttgcttgcctcatatacttggtctgacgaatccttgcttttcttgggagcttctgatgaagagttgaaggagttggcccttagagacttggcaaaaattcacggtgaacaagtttgggataagtgcacaggagttatcgtcaagaaatggtctgctgacccatactccttgggtgcatttgctcttttcaccccttaccaacatttggaatatgcacaggagttgttttcttctgaaggtagagttcattttgctggagagcacaccgcattccctcatgcttggattgaaacttcaatgaaaagtgctatcagagctgccacaaacattaacaaagtcgctaacgaagaatccaccattgaacacacaaaagatgagttgtagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2027051_sequence 1 atggagcacttggctgattgcttggaagataaggattacgatacacttttgcagacattggacaacggtttgcctcacattaacacttctcatcacgttgtcattgttggtgccggaatggcaggtttgaccgctgccaaattgcttcaagatgcaggacacactgttacaatcttggaggctaacgacagagttggtggaagagtcgaaacttacagaaatgaaaaggagggttggtatgctgaaatgggagccatgagaatcccatcttctcatagaatcgttcagtggtttgtcaagaaattgggtgttgaaatgaacgagtttgtcatgacagatgacaacaccttctacttggttaatggagtcagagaaagaacttatgttgtccaagagaaccctgatgttttgaagtacaatgtctctgaatccgagaaaggtatttctgctgatgacttgcttgacagagccttgcagaaggttaaagaagaggtcgaagctaatggttgtaaggcagctttggagaagtacgatagatactctgttaaggaatatttgaaagaagagggtggactttccccaggtgctgttagaatgattggagatttgcttaacgagcaatctttgatgtacactgccctttccgaaatgatctatgatcaggcagacgttaatgattcagtcagttaccacgaggttacaggtggatccgatttgcttccagaagctttcttgtcagttcttgacgtccctatcttgcttaactccaaggttaagcatatcagacaatcagataagggtgttatcgtcagttatcagactggaaatgaatcaagtttgatggacctttctgctgatattgttttggtcactacaaccgccaaagccgcattgtttattgatttcgacccacctttgtctatctccaagatggaggctttgagatctgttcactacgattcttccactaagatcttgcttacttttagagacaagttctgggaagatgacggtattagaggtggaaagtcaatcacagatggaccaagtagatacatctactatccttcacatagttttcacaccaacgagactatcggtgttttgcttgcctcatatacttggtctgacgaatccttgcttttcttgggagcttctgatgaagagttgaaggagttggcccttagagacttggcaaaaattcacggtgaacaagtttgggataagtgcacaggagttatcgtcaagaaatggtctgctgacccatactccttgggtgcatttgctcttttcaccccttaccaacatttggaatatgcacaggagttgttttcttctgaaggtagagttcattttgctggagagcacaccgcattccctcatgcttggattgaaacttcaatgaaaagtgctatcagagctgccacaaacattaacaaagtcgctaacgaagaatccaccattgaacacacaaaagatgagttgtag BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z