BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K2027052 1 BBa_K2027052 Flag-Lumio-HisTag 2016-10-23T11:00:00Z 2016-10-24T01:41:34Z See BBa_K2027000 Scar sequence between T7 Promoter and RBS: TACTAGAG false false _2494_ 29123 29123 9 false Necessary spacing false Michael Becich BBa_K2027040 1 BBa_K2027040 DXS Synthase protein (G3P +Pyr->DXP) 2016-10-12T11:00:00Z 2016-10-25T09:29:46Z TBA TBA false false _2494_ 31619 29123 9 false TBA false Michael Becich component2530236 1 BBa_B0010 component2530238 1 BBa_B0012 component2530233 1 BBa_B0034 component2530231 1 BBa_Z0251 component2530234 1 BBa_K2027056 component2530235 1 BBa_K2027052 annotation2530235 1 BBa_K2027052 range2530235 1 1930 2001 annotation2530238 1 BBa_B0012 range2530238 1 2098 2138 annotation2530236 1 BBa_B0010 range2530236 1 2010 2089 annotation2530233 1 BBa_B0034 range2530233 1 44 55 annotation2530234 1 BBa_K2027056 range2530234 1 62 1921 annotation2530231 1 BBa_Z0251 range2530231 1 1 35 BBa_K2027056 1 BBa_K2027056 <i>E. coli</i> DXS Synthase Enzyme cds (codon optimized) 2016-10-23T11:00:00Z 2016-10-24T02:34:04Z Sequence is sourced from Zhao Y, Yang J, Qin B, et al. Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway. Appl Microbiol Biotechnol. 2011;90(6):1915-22. (http://www.ecogene.org/gene/EG13612/dnasequence?q=gene/EG13612/dnasequence&sites[0]=BamHI&sites[1]=EcoRI&sites[2]=HindIII&left_end=433315&right_end=443315&type=fasta) EcoGene: EG13612 This sequence codes for DXS synthase, an enzyme involved in the conversion of G3P and Pyruvate to DXP in the MEP/DOXP pathway. Because DXS synthase activity is the rate limiting step in the MEP/DOXP pathway, overexpression of this gene will override the bottleneck in the G3P and Pyr conversion process. This sequence is endogenous to <i>E. coli</i> and is referenced from EcoGene:EG13612. false false _2494_ 31619 31619 9 false This sequence has been codon optimized for expression in <i>E. coli</i>. No further modifications were made. false Gordon Sun BBa_Z0251 1 BBa_Z0251 T7 strong promoter 2005-04-04T11:00:00Z 2015-05-08T01:14:55Z bacteriophage T7 Promoter for T7 RNA polymerase false false _10_ 0 250 10 Not in stock false false T7.2 annotation1476333 1 consensus sequence range1476333 1 6 28 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K2027040_sequence 1 gaaattaatacgactcactatagggagaccacaactactagagaaagaggagaaatactagatgagttttgacattgcgaagtaccctacattggcgcttgtggacagcactcaagagttgcgtttgttgccgaaggagagcttgcctaaactgtgtgacgagctgcgccgttacttattggatagtgtgtctcgctcttcgggtcattttgcgtcgggtctgggtactgtggaacttacggtggcgctgcattacgtatacaataccccctttgatcagctgatctgggatgtaggacatcaagcttatccgcataagatcttaactggtcgccgtgacaaaattggcacgatccgtcaaaaaggtggcttacatccgttcccgtggcgcggcgaaagtgagtacgatgtactgtcggtgggccattcgtctacatctatttcggctggtatcggtatcgcggtagccgcagagaaggagggaaaaaatcgtcgcacagtatgtgttatcggtgatggagctattacagcgggtatggccttcgaggccatgaaccacgccggagacattcgtcccgatatgttagtaattttgaatgacaatgaaatgtctattagtgagaatgtcggggcgttaaacaatcatctggctcaattattatcaggtaaactttattcatcgctgcgtgagggtggtaagaaggttttttctggagtgccacccattaaggagttattaaagcgcacggaagagcacattaaaggaatggtggtgccagggacgttgtttgaggagttgggtttcaactacattggtccagtagacggccatgatgtccttgggcttatcacgacattaaaaaatatgcgcgatttgaaaggcccgcagttcctgcatatcatgaccaaaaaggggcgcggttatgagcctgctgagaaagaccccattacttttcatgcggttccaaaatttgatccatcgtcggggtgcctgcctaaaagctctggaggactgccttcatatagcaaaatctttggagattggttatgtgagacagctgccaaagataataaattaatggcgattacgcctgcgatgcgtgaagggtcagggatggtcgagttctcgcgtaagtttcccgatcgttactttgacgttgccatcgctgagcaacacgctgtgaccttcgcagctggattagcgattggtgggtacaaaccgattgttgctatctattccacttttttacagcgcgcatacgatcaagttttacacgatgtcgcaatccagaagttgcccgtcttgtttgccattgatcgtgcgggtatcgtaggggcagacggtcaaacacaccagggcgcatttgatttgagctatcttcgttgtatcccggaaatggttattatgaccccctcggatgaaaatgagtgtcgtcaaatgttatatactgggtaccattacaacgatggcccatcggcagtgcgttatccccgtggaaatgcggtcggtgtagagttaaccccccttgagaagctgcctattggaaaagggatcgttaagcgccgcggggagaaattggcaattctgaacttcggtacacttatgccagaggctgcgaaagttgcagaatccttgaatgctaccttggtcgacatgcgcttcgttaagcccttggatgaagctcttatcttggagatggcggcaagtcacgaagcgttggtgactgtagaggagaacgctatcatgggtggagctgggagcggggttaatgaggtgttaatggcccatcgtaagcctgttcctgtgttgaatattggattgccggatttcttcatcccacaaggtacgcaagaagagatgcgcgccgaattgggcttagacgcagcaggcatggaagcaaagattaaggcttggttagcctactagaggactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2027056_sequence 1 atgagttttgacattgcgaagtaccctacattggcgcttgtggacagcactcaagagttgcgtttgttgccgaaggagagcttgcctaaactgtgtgacgagctgcgccgttacttattggatagtgtgtctcgctcttcgggtcattttgcgtcgggtctgggtactgtggaacttacggtggcgctgcattacgtatacaataccccctttgatcagctgatctgggatgtaggacatcaagcttatccgcataagatcttaactggtcgccgtgacaaaattggcacgatccgtcaaaaaggtggcttacatccgttcccgtggcgcggcgaaagtgagtacgatgtactgtcggtgggccattcgtctacatctatttcggctggtatcggtatcgcggtagccgcagagaaggagggaaaaaatcgtcgcacagtatgtgttatcggtgatggagctattacagcgggtatggccttcgaggccatgaaccacgccggagacattcgtcccgatatgttagtaattttgaatgacaatgaaatgtctattagtgagaatgtcggggcgttaaacaatcatctggctcaattattatcaggtaaactttattcatcgctgcgtgagggtggtaagaaggttttttctggagtgccacccattaaggagttattaaagcgcacggaagagcacattaaaggaatggtggtgccagggacgttgtttgaggagttgggtttcaactacattggtccagtagacggccatgatgtccttgggcttatcacgacattaaaaaatatgcgcgatttgaaaggcccgcagttcctgcatatcatgaccaaaaaggggcgcggttatgagcctgctgagaaagaccccattacttttcatgcggttccaaaatttgatccatcgtcggggtgcctgcctaaaagctctggaggactgccttcatatagcaaaatctttggagattggttatgtgagacagctgccaaagataataaattaatggcgattacgcctgcgatgcgtgaagggtcagggatggtcgagttctcgcgtaagtttcccgatcgttactttgacgttgccatcgctgagcaacacgctgtgaccttcgcagctggattagcgattggtgggtacaaaccgattgttgctatctattccacttttttacagcgcgcatacgatcaagttttacacgatgtcgcaatccagaagttgcccgtcttgtttgccattgatcgtgcgggtatcgtaggggcagacggtcaaacacaccagggcgcatttgatttgagctatcttcgttgtatcccggaaatggttattatgaccccctcggatgaaaatgagtgtcgtcaaatgttatatactgggtaccattacaacgatggcccatcggcagtgcgttatccccgtggaaatgcggtcggtgtagagttaaccccccttgagaagctgcctattggaaaagggatcgttaagcgccgcggggagaaattggcaattctgaacttcggtacacttatgccagaggctgcgaaagttgcagaatccttgaatgctaccttggtcgacatgcgcttcgttaagcccttggatgaagctcttatcttggagatggcggcaagtcacgaagcgttggtgactgtagaggagaacgctatcatgggtggagctgggagcggggttaatgaggtgttaatggcccatcgtaagcctgttcctgtgttgaatattggattgccggatttcttcatcccacaaggtacgcaagaagagatgcgcgccgaattgggcttagacgcagcaggcatggaagcaaagattaaggcttggttagcc BBa_B0034_sequence 1 aaagaggagaaa BBa_Z0251_sequence 1 gaaattaatacgactcactatagggagaccacaac BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2027052_sequence 1 gactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z