BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K2027052
1
BBa_K2027052
Flag-Lumio-HisTag
2016-10-23T11:00:00Z
2016-10-24T01:41:34Z
See BBa_K2027000
Scar sequence between T7 Promoter and RBS: TACTAGAG
false
false
_2494_
29123
29123
9
false
Necessary spacing
false
Michael Becich
BBa_K2027040
1
BBa_K2027040
DXS Synthase protein (G3P +Pyr->DXP)
2016-10-12T11:00:00Z
2016-10-25T09:29:46Z
TBA
TBA
false
false
_2494_
31619
29123
9
false
TBA
false
Michael Becich
component2530236
1
BBa_B0010
component2530238
1
BBa_B0012
component2530233
1
BBa_B0034
component2530231
1
BBa_Z0251
component2530234
1
BBa_K2027056
component2530235
1
BBa_K2027052
annotation2530235
1
BBa_K2027052
range2530235
1
1930
2001
annotation2530238
1
BBa_B0012
range2530238
1
2098
2138
annotation2530236
1
BBa_B0010
range2530236
1
2010
2089
annotation2530233
1
BBa_B0034
range2530233
1
44
55
annotation2530234
1
BBa_K2027056
range2530234
1
62
1921
annotation2530231
1
BBa_Z0251
range2530231
1
1
35
BBa_K2027056
1
BBa_K2027056
<i>E. coli</i> DXS Synthase Enzyme cds (codon optimized)
2016-10-23T11:00:00Z
2016-10-24T02:34:04Z
Sequence is sourced from Zhao Y, Yang J, Qin B, et al. Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway. Appl Microbiol Biotechnol. 2011;90(6):1915-22. (http://www.ecogene.org/gene/EG13612/dnasequence?q=gene/EG13612/dnasequence&sites[0]=BamHI&sites[1]=EcoRI&sites[2]=HindIII&left_end=433315&right_end=443315&type=fasta)
EcoGene: EG13612
This sequence codes for DXS synthase, an enzyme involved in the conversion of G3P and Pyruvate to DXP in the MEP/DOXP pathway. Because DXS synthase activity is the rate limiting step in the MEP/DOXP pathway, overexpression of this gene will override the bottleneck in the G3P and Pyr conversion process.
This sequence is endogenous to <i>E. coli</i> and is referenced from EcoGene:EG13612.
false
false
_2494_
31619
31619
9
false
This sequence has been codon optimized for expression in <i>E. coli</i>.
No further modifications were made.
false
Gordon Sun
BBa_Z0251
1
BBa_Z0251
T7 strong promoter
2005-04-04T11:00:00Z
2015-05-08T01:14:55Z
bacteriophage T7
Promoter for T7 RNA polymerase
false
false
_10_
0
250
10
Not in stock
false
false
T7.2
annotation1476333
1
consensus sequence
range1476333
1
6
28
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K2027040_sequence
1
gaaattaatacgactcactatagggagaccacaactactagagaaagaggagaaatactagatgagttttgacattgcgaagtaccctacattggcgcttgtggacagcactcaagagttgcgtttgttgccgaaggagagcttgcctaaactgtgtgacgagctgcgccgttacttattggatagtgtgtctcgctcttcgggtcattttgcgtcgggtctgggtactgtggaacttacggtggcgctgcattacgtatacaataccccctttgatcagctgatctgggatgtaggacatcaagcttatccgcataagatcttaactggtcgccgtgacaaaattggcacgatccgtcaaaaaggtggcttacatccgttcccgtggcgcggcgaaagtgagtacgatgtactgtcggtgggccattcgtctacatctatttcggctggtatcggtatcgcggtagccgcagagaaggagggaaaaaatcgtcgcacagtatgtgttatcggtgatggagctattacagcgggtatggccttcgaggccatgaaccacgccggagacattcgtcccgatatgttagtaattttgaatgacaatgaaatgtctattagtgagaatgtcggggcgttaaacaatcatctggctcaattattatcaggtaaactttattcatcgctgcgtgagggtggtaagaaggttttttctggagtgccacccattaaggagttattaaagcgcacggaagagcacattaaaggaatggtggtgccagggacgttgtttgaggagttgggtttcaactacattggtccagtagacggccatgatgtccttgggcttatcacgacattaaaaaatatgcgcgatttgaaaggcccgcagttcctgcatatcatgaccaaaaaggggcgcggttatgagcctgctgagaaagaccccattacttttcatgcggttccaaaatttgatccatcgtcggggtgcctgcctaaaagctctggaggactgccttcatatagcaaaatctttggagattggttatgtgagacagctgccaaagataataaattaatggcgattacgcctgcgatgcgtgaagggtcagggatggtcgagttctcgcgtaagtttcccgatcgttactttgacgttgccatcgctgagcaacacgctgtgaccttcgcagctggattagcgattggtgggtacaaaccgattgttgctatctattccacttttttacagcgcgcatacgatcaagttttacacgatgtcgcaatccagaagttgcccgtcttgtttgccattgatcgtgcgggtatcgtaggggcagacggtcaaacacaccagggcgcatttgatttgagctatcttcgttgtatcccggaaatggttattatgaccccctcggatgaaaatgagtgtcgtcaaatgttatatactgggtaccattacaacgatggcccatcggcagtgcgttatccccgtggaaatgcggtcggtgtagagttaaccccccttgagaagctgcctattggaaaagggatcgttaagcgccgcggggagaaattggcaattctgaacttcggtacacttatgccagaggctgcgaaagttgcagaatccttgaatgctaccttggtcgacatgcgcttcgttaagcccttggatgaagctcttatcttggagatggcggcaagtcacgaagcgttggtgactgtagaggagaacgctatcatgggtggagctgggagcggggttaatgaggtgttaatggcccatcgtaagcctgttcctgtgttgaatattggattgccggatttcttcatcccacaaggtacgcaagaagagatgcgcgccgaattgggcttagacgcagcaggcatggaagcaaagattaaggcttggttagcctactagaggactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K2027056_sequence
1
atgagttttgacattgcgaagtaccctacattggcgcttgtggacagcactcaagagttgcgtttgttgccgaaggagagcttgcctaaactgtgtgacgagctgcgccgttacttattggatagtgtgtctcgctcttcgggtcattttgcgtcgggtctgggtactgtggaacttacggtggcgctgcattacgtatacaataccccctttgatcagctgatctgggatgtaggacatcaagcttatccgcataagatcttaactggtcgccgtgacaaaattggcacgatccgtcaaaaaggtggcttacatccgttcccgtggcgcggcgaaagtgagtacgatgtactgtcggtgggccattcgtctacatctatttcggctggtatcggtatcgcggtagccgcagagaaggagggaaaaaatcgtcgcacagtatgtgttatcggtgatggagctattacagcgggtatggccttcgaggccatgaaccacgccggagacattcgtcccgatatgttagtaattttgaatgacaatgaaatgtctattagtgagaatgtcggggcgttaaacaatcatctggctcaattattatcaggtaaactttattcatcgctgcgtgagggtggtaagaaggttttttctggagtgccacccattaaggagttattaaagcgcacggaagagcacattaaaggaatggtggtgccagggacgttgtttgaggagttgggtttcaactacattggtccagtagacggccatgatgtccttgggcttatcacgacattaaaaaatatgcgcgatttgaaaggcccgcagttcctgcatatcatgaccaaaaaggggcgcggttatgagcctgctgagaaagaccccattacttttcatgcggttccaaaatttgatccatcgtcggggtgcctgcctaaaagctctggaggactgccttcatatagcaaaatctttggagattggttatgtgagacagctgccaaagataataaattaatggcgattacgcctgcgatgcgtgaagggtcagggatggtcgagttctcgcgtaagtttcccgatcgttactttgacgttgccatcgctgagcaacacgctgtgaccttcgcagctggattagcgattggtgggtacaaaccgattgttgctatctattccacttttttacagcgcgcatacgatcaagttttacacgatgtcgcaatccagaagttgcccgtcttgtttgccattgatcgtgcgggtatcgtaggggcagacggtcaaacacaccagggcgcatttgatttgagctatcttcgttgtatcccggaaatggttattatgaccccctcggatgaaaatgagtgtcgtcaaatgttatatactgggtaccattacaacgatggcccatcggcagtgcgttatccccgtggaaatgcggtcggtgtagagttaaccccccttgagaagctgcctattggaaaagggatcgttaagcgccgcggggagaaattggcaattctgaacttcggtacacttatgccagaggctgcgaaagttgcagaatccttgaatgctaccttggtcgacatgcgcttcgttaagcccttggatgaagctcttatcttggagatggcggcaagtcacgaagcgttggtgactgtagaggagaacgctatcatgggtggagctgggagcggggttaatgaggtgttaatggcccatcgtaagcctgttcctgtgttgaatattggattgccggatttcttcatcccacaaggtacgcaagaagagatgcgcgccgaattgggcttagacgcagcaggcatggaagcaaagattaaggcttggttagcc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_Z0251_sequence
1
gaaattaatacgactcactatagggagaccacaac
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K2027052_sequence
1
gactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z