BBa_K2027053
1
BBa_K2027053
<i>H. Brasiliensis</i> SRPP cDNA (codon Optimized)
2016-10-23T11:00:00Z
2016-10-24T02:36:47Z
Sequence was sourced from Oh SK, Kang H, Shin DH, et al. Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis. J Biol Chem. 1999;274(24):17132-8. (http://www.ncbi.nlm.nih.gov/nuccore/AF051317)
This sequence contains the gene for a small rubber particle protein (SRPP) from the rubber tree Hevea brasiliensis. This coenzyme serves as an activator for rubber transferases/prenyltransferases in H. brasiliensis. This protein is involved in rubber elongation.
Characteristics:
??? 612bp ORF
??? 63-bp 59-UTR
??? 181-bp 39-UTR
??? 18bp poly A tail
??? Localized in the cytoplasm
false
false
_2494_
31619
31619
9
false
This sequence has been codon optimized for transfection into E. coli.
Mutation A486T was induced to remove a restriction site.
A FLAG-Lumio tag can be appended to the sequence to allow for visualization and/or purification.
false
Gordon Sun
BBa_K2027053_sequence
1
atggccgaagaggtagaggaagagcgcctgaaatatctggactttgtacgcgcagctggagtctatgcggttgattcattctctacgttgtacttgtatgccaaggacatttcaggcccattgaaacctggggtagacaccatcgagaatgtcgtcaagacggtagttaccccggtgtactatatccctttagaagcagttaagtttgtcgacaaaacggttgatgttagtgttacgagtttagacggcgttgtgccgccagtaattaagcaagtatcggcacagacctacagcgtcgcgcaggatgctccccgcattgtgttagatgtcgcttcttctgtttttaacacgggcgttcaggaaggagcaaaagcgctgtacgcaaaccttgagccaaaggccgaacagtacgcggttatcacatggcgcgcacttaataaacttcctcttgtcccacaagttgcgaatgtcgttgtaccgactgctgtttattttagcgaaaaatataacgatgttgtacgtggcactaccgagcaaggctaccgtgttagctcgtaccttcctctgttacctacggaaaaaatcacgaaggtattcggagacgaggcatca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z