BBa_K2027056 1 BBa_K2027056 <i>E. coli</i> DXS Synthase Enzyme cds (codon optimized) 2016-10-23T11:00:00Z 2016-10-24T02:34:04Z Sequence is sourced from Zhao Y, Yang J, Qin B, et al. Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway. Appl Microbiol Biotechnol. 2011;90(6):1915-22. (http://www.ecogene.org/gene/EG13612/dnasequence?q=gene/EG13612/dnasequence&sites[0]=BamHI&sites[1]=EcoRI&sites[2]=HindIII&left_end=433315&right_end=443315&type=fasta) EcoGene: EG13612 This sequence codes for DXS synthase, an enzyme involved in the conversion of G3P and Pyruvate to DXP in the MEP/DOXP pathway. Because DXS synthase activity is the rate limiting step in the MEP/DOXP pathway, overexpression of this gene will override the bottleneck in the G3P and Pyr conversion process. This sequence is endogenous to <i>E. coli</i> and is referenced from EcoGene:EG13612. false false _2494_ 31619 31619 9 false This sequence has been codon optimized for expression in <i>E. coli</i>. No further modifications were made. false Gordon Sun BBa_K2027056_sequence 1 atgagttttgacattgcgaagtaccctacattggcgcttgtggacagcactcaagagttgcgtttgttgccgaaggagagcttgcctaaactgtgtgacgagctgcgccgttacttattggatagtgtgtctcgctcttcgggtcattttgcgtcgggtctgggtactgtggaacttacggtggcgctgcattacgtatacaataccccctttgatcagctgatctgggatgtaggacatcaagcttatccgcataagatcttaactggtcgccgtgacaaaattggcacgatccgtcaaaaaggtggcttacatccgttcccgtggcgcggcgaaagtgagtacgatgtactgtcggtgggccattcgtctacatctatttcggctggtatcggtatcgcggtagccgcagagaaggagggaaaaaatcgtcgcacagtatgtgttatcggtgatggagctattacagcgggtatggccttcgaggccatgaaccacgccggagacattcgtcccgatatgttagtaattttgaatgacaatgaaatgtctattagtgagaatgtcggggcgttaaacaatcatctggctcaattattatcaggtaaactttattcatcgctgcgtgagggtggtaagaaggttttttctggagtgccacccattaaggagttattaaagcgcacggaagagcacattaaaggaatggtggtgccagggacgttgtttgaggagttgggtttcaactacattggtccagtagacggccatgatgtccttgggcttatcacgacattaaaaaatatgcgcgatttgaaaggcccgcagttcctgcatatcatgaccaaaaaggggcgcggttatgagcctgctgagaaagaccccattacttttcatgcggttccaaaatttgatccatcgtcggggtgcctgcctaaaagctctggaggactgccttcatatagcaaaatctttggagattggttatgtgagacagctgccaaagataataaattaatggcgattacgcctgcgatgcgtgaagggtcagggatggtcgagttctcgcgtaagtttcccgatcgttactttgacgttgccatcgctgagcaacacgctgtgaccttcgcagctggattagcgattggtgggtacaaaccgattgttgctatctattccacttttttacagcgcgcatacgatcaagttttacacgatgtcgcaatccagaagttgcccgtcttgtttgccattgatcgtgcgggtatcgtaggggcagacggtcaaacacaccagggcgcatttgatttgagctatcttcgttgtatcccggaaatggttattatgaccccctcggatgaaaatgagtgtcgtcaaatgttatatactgggtaccattacaacgatggcccatcggcagtgcgttatccccgtggaaatgcggtcggtgtagagttaaccccccttgagaagctgcctattggaaaagggatcgttaagcgccgcggggagaaattggcaattctgaacttcggtacacttatgccagaggctgcgaaagttgcagaatccttgaatgctaccttggtcgacatgcgcttcgttaagcccttggatgaagctcttatcttggagatggcggcaagtcacgaagcgttggtgactgtagaggagaacgctatcatgggtggagctgggagcggggttaatgaggtgttaatggcccatcgtaagcctgttcctgtgttgaatattggattgccggatttcttcatcccacaaggtacgcaagaagagatgcgcgccgaattgggcttagacgcagcaggcatggaagcaaagattaaggcttggttagcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z