BBa_K2029000 1 nprb P43_RBS_nprb 2016-08-28T11:00:00Z 2016-08-29T12:12:46Z p43_RBS_nprb sequence was taken from the p43NMK plasmid: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1169017/ Part consisting of the P43 promotor, RBS and coding sequence of the nprb signal peptide which induces secretion in B. subtilis. This part can be used to produce and secrete proteins in B. subtilis. false false _2496_ 27381 27381 9 false standard iGEM cloning site was replaced with analoge for scarless cloning false Max Schelski, Niklas Schmacke annotation2481961 1 start codon range2481961 1 300 302 annotation2481960 1 RBS range2481960 1 281 290 annotation2481959 1 p43 promotor range2481959 1 1 280 annotation2481962 1 nprb signal peptide range2481962 1 303 380 BBa_K2029002 1 BBa_K2029002 XynA 2016-10-13T11:00:00Z 2016-10-30T09:03:31Z https://www.ncbi.nlm.nih.gov/pubmed/22101447 Catalytic enzyme (xylanase) false false _2496_ 28072 29985 9 false This part was designed for use in Bacillus subtilis false Niklas Schmacke, Max Schelski annotation2531377 1 Transcription start range2531377 1 46 48 annotation2531378 1 XynA-Protein range2531378 1 46 651 annotation2531380 1 His tag range2531380 1 634 651 annotation2531379 1 TT range2531379 1 652 657 BBa_K2029010 1 BBa_K2029010 nprb_XynA 2016-10-13T11:00:00Z 2016-10-14T03:33:14Z Enzyme: http://www.ebi.ac.uk/ena/data/view/M36648&display=fasta Catalytic enzyme (xylanase) preceded by secretion tag true false _2496_ 29985 29985 9 false Designed for use in Bacillus subtilis C-terminal 6His-tag added for convenient purification false Bui Hoang Duy component2509715 1 BBa_K2029002 component2509714 1 BBa_K2029000 annotation2509715 1 BBa_K2029002 range2509715 1 395 1168 annotation2509714 1 BBa_K2029000 range2509714 1 1 386 BBa_K2029010_sequence 1 tgataggtggtatgttttcgcttgaacttttaaatacagccattgaacatacggttgatttaataactgacaaacatcaccctcttgctaaagcggccaaggacgctgccgccggggctgtttgcgtttttgccgtgatttcgtgtatcattggtttacttatttttttgccaaagctgtaatggctgaaaattcttacatttattttacatttttagaaatgggcgtgaaaaaaagcgcgcgattatgtaaaatataaagtgatagcggtaccattatagtaagagaggaatgtacacatgcgcaacttgaccaagacatctctattactggccggcttatgcacagcggcccaaatggtttttgtaacacatgcctcagctagctactagagtttaagtttaaaaagaatttcctagttggattatcggcagctttaatgagtattagcttgttttcggcaaccgcctccgcagcaagcacagactactggcaaaattggactgatgggggcggtatagtaaacgctgtcaatgggtctggcgggaattacagtgttaattggtctaataccggaaattttgttgttggtaaaggttggactacaggctcgccatttaggacgataaactataatgccggagtttgggcgccgaatggcaatggatatttaactttatatggttggacaagatcacctctcatagaatattatgtagtggattcatggggtacttatagacctactggaacgtataaaggtactgtaaaaagtgatgggggtacgtatgacatatatacaactacacgttataacgcaccttccattgatggagatcgcactacttttacgcagtactggagtgttcgccagtcgaaaagaccaaccggaagcaacgcaacaatcactttcagcaatcatgtgaacgcatggaagtcacatggaatgaatctgggcagtaattgggcttatcaagtcatggcgacagaaggatatcaaagtagtggatcatctaacgtaacagtgcaccaccaccaccaccactaataaactagattgttcagaacgctcggtcttgcacaccgggcgttttttctttgtgagtccaactagataatttataaataaaaatcaccttttagaggtggtttttttatttataaatta BBa_K2029000_sequence 1 tgataggtggtatgttttcgcttgaacttttaaatacagccattgaacatacggttgatttaataactgacaaacatcaccctcttgctaaagcggccaaggacgctgccgccggggctgtttgcgtttttgccgtgatttcgtgtatcattggtttacttatttttttgccaaagctgtaatggctgaaaattcttacatttattttacatttttagaaatgggcgtgaaaaaaagcgcgcgattatgtaaaatataaagtgatagcggtaccattatagtaagagaggaatgtacacatgcgcaacttgaccaagacatctctattactggccggcttatgcacagcggcccaaatggtttttgtaacacatgcctcagctagc BBa_K2029002_sequence 1 tttaagtttaaaaagaatttcctagttggattatcggcagctttaatgagtattagcttgttttcggcaaccgcctccgcagcaagcacagactactggcaaaattggactgatgggggcggtatagtaaacgctgtcaatgggtctggcgggaattacagtgttaattggtctaataccggaaattttgttgttggtaaaggttggactacaggctcgccatttaggacgataaactataatgccggagtttgggcgccgaatggcaatggatatttaactttatatggttggacaagatcacctctcatagaatattatgtagtggattcatggggtacttatagacctactggaacgtataaaggtactgtaaaaagtgatgggggtacgtatgacatatatacaactacacgttataacgcaccttccattgatggagatcgcactacttttacgcagtactggagtgttcgccagtcgaaaagaccaaccggaagcaacgcaacaatcactttcagcaatcatgtgaacgcatggaagtcacatggaatgaatctgggcagtaattgggcttatcaagtcatggcgacagaaggatatcaaagtagtggatcatctaacgtaacagtgcaccaccaccaccaccactaataaactagattgttcagaacgctcggtcttgcacaccgggcgttttttctttgtgagtccaactagataatttataaataaaaatcaccttttagaggtggtttttttatttataaatta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z