LOCUS BBa_K2029015 5434 bp DNA linear 28-Sep-2024 DEFINITION pSBbs1AK1 (replicative Bacillus subtilis expression vector) ACCESSION BBa_K2029015 VERSION BBa_K2029015.1 FEATURES Location/Qualifiers misc_feature 264..878 /label=pMB1 Ori (Coli) misc_feature 2436..3441 /label=repU Ori (subtilis) CDS 3600..4370 /label=KanR CDS 1039..1698 /label=AmpR primer_bind 2012..2020 /label=pBR1 CDS 4593..4991 /label=ble ORIGIN 1 actagtagcg gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt 61 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg 121 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacaagct 181 tttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 241 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga 301 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 361 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 421 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 481 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 541 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 601 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 661 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 721 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 781 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 841 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 901 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 961 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 1021 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 1081 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 1141 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 1201 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 1261 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 1321 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 1381 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 1441 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 1501 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 1561 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 1621 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 1681 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 1741 accgctgttg agatccagtt cgatataacc cactcgtgca cccaactgat cttcagcatc 1801 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 1861 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 1921 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 1981 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg gagctcaagg aacgtacaga 2041 cggcttaaaa gcctttaaaa acgtttttaa ggggtttgta gacaaggtaa aggataaaac 2101 agcacaattc caagaaaaac acgatttaga acctaaaaag aacgaatttg aactaactca 2161 taaccgagag gtaaaaaaag aacgaagtcg agatcaggga atgagtttat aaaataaaaa 2221 aagcacctga aaaggtgtct ttttttgatg gttttgaact tgttctttct tatcttgata 2281 catatagaaa taacgtcatt tttattttag ttgctgaaag gtgcgttgaa gtgttggtat 2341 gtatgtgttt taaagtattg aaaaccctta aaattggttg cacagaaaaa ccccatctgt 2401 taaagttata agtgactaaa caaataacta aatagatggg ggtttctttt aatattatgt 2461 gtcctaatag tagcatttat tcagatgaaa aatcaagggt tttagtggac aagacaaaaa 2521 gtggaaaagt gagaccatgg agagaaaaga aaatcgctaa tgttgattac tttgaacttc 2581 tgcatattct tgaatttaaa aaggctgaaa gagtaaaaga ttgtgctgaa atattagagt 2641 ataaacaaaa tcgtgaaaca ggcgaaagaa agttgtatcg agtgtggttt tgtaaatcca 2701 ggctttgtcc aatgtgcaac tggaggagag caatgaaaca tggcattcag tcacaaaagg 2761 ttgttgctga agttattaaa caaaagccaa cagttcgttg gttgtttctc acattaacag 2821 ttaaaaatgt ttatgatggc gaagaattaa ataagagttt gtcagatatg gctcaaggat 2881 ttcgccgaat gatgcaatat aaaaaaatta ataaaaatct tgttggtttt atgcgtgcaa 2941 cggaagtgac aataaataat aaagataatt cttataatca gcacatgcat gtattggtat 3001 gtgtggaacc aacttatttt aagaatacag aaaactacgt gaatcaaaaa caatggattc 3061 aattttggaa aaaggcaatg aaattagact atgatccaaa tgtaaaagtt caaatgattc 3121 gaccgaaaaa taaatataaa tcggatatac aatcggcaat tgacgaaact gcaaaatatc 3181 ctgtaaagga tacggatttt atgaccgatg atgaagaaaa gaatttgaaa cgtttgtctg 3241 atttggagga aggtttacac cgtaaaaggt taatctccta tggtggtttg ttaaaagaaa 3301 tacataaaaa attaaacctt gatgacacag aagaaggcga tttgattcat acagatgatg 3361 acgaaaaagc cgatgaagat ggattttcta ttattgcaat gtggaattgg gaacggaaaa 3421 attattttat taaagagtag ttcaacaaac gggccagttt gttgaagatt agatgctata 3481 attgttatta aaaggattga aggatgctta ggaagacgag ttattaatag ctgaataaga 3541 acggtgctct ccaaatattc ttatttagaa aagcaaatct aaaattatct gaaaagggaa 3601 tgagaatagt gaatggacca ataataatga ctagagaaga aagaatgaag attgttcatg 3661 aaattaagga acgaatattg gataaatatg gggatgatgt taaggctatt ggtgtttatg 3721 gctctcttgg tcgtcagact gatgggccct attcggatat tgagatgatg tgtgtcatgt 3781 caacagagga agcagagttc agccatgaat ggacaaccgg tgagtggaag gtggaagtga 3841 attttgatag cgaagagatt ctactagatt atgcatctca ggtggaatca gattggccgc 3901 ttacacatgg tcaatttttc tctattttgc cgatttatga ttcaggtgga tacttagaga 3961 aagtgtatca aactgctaaa tcggtagaag cccaaacgtt ccacgatgcg atttgtgccc 4021 ttatcgtaga agagctgttt gaatatgcag gcaaatggcg taatattcgt gtgcaaggac 4081 cgacaacatt tctaccatcc ttgactgtac aggtagcaat ggcaggtgcc atgttgattg 4141 gtctgcatca tcgcatctgt tatacgacga gcgcttcggt cttaactgaa gcagttaagc 4201 aatcagatct tccttcaggt tatgaccatc tgtgccagtt cgtaatgtct ggtcaacttt 4261 ccgactctga gaaacttctg gaatcgctag agaatttctg gaatgggatt caggagtgga 4321 cagaacgaca cggatatata gtggatgtgt caaaacgcat accattttga acgatgacct 4381 ctaataattg ttaatcatgt tggttacgta tttattaact tctcctagta ttagtaatta 4441 tcatggctgt catggcgcat taacggaata aagggtgtgc ttaaatcggg ccattttgcg 4501 taataagaaa aaggattaat tatgagcgaa ttgaattaat aataaggtaa tagatttaca 4561 ttagaaaatg aaaggggatt ttatgcgtga gaatgttaca gtctatcccg gcattgccag 4621 tcggggatat taaaaagagt ataggttttt attgcgataa actaggtttc actttggttc 4681 accatgaaga tggattcgca gttctaatgt gtaatgaggt tcggattcat ctatgggagg 4741 caagtgatga aggctggcgc tctcgtagta atgattcacc ggtttgtaca ggtgcggagt 4801 cgtttattgc tggtactgct agttgccgca ttgaagtaga gggaattgat gaattatatc 4861 aacatattaa gcctttgggc attttgcacc ccaatacatc attaaaagat cagtggtggg 4921 atgaacgaga ctttgcagta attgatcccg acaacaattt gattagcttt tttcaacaaa 4981 taaaaagcta aaatctatta ttaatctgtt cagcaatcgg gcgcgattgc tgaataaaag 5041 atacgagaga cctctcttgt atctttttta ttttgagtgg ttttgtccgt tacactagaa 5101 aaccgaaaga caataaaaat tttattcttg ctgagtctgg ctttcggtaa gctagacaaa 5161 acggacaaaa taaaaattgg caagggttta aaggtggaga ttttttgagt gatcttctca 5221 aaaaatacta cctgtccctt gctgattttt aaacgagcac gagagcaaaa cccccctttg 5281 ctgaggtggc gggtacccca cctgacgtct aagaaaccat tattatcatg acattaacct 5341 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta 5401 aggatgattt ctggaattcg cggccgcttc taga //