BBa_K2030002 1 BBa_K2030002 Acetate kinase (<i>ackA</i>) from Synechocystis 2016-08-16T11:00:00Z 2016-10-19T06:45:35Z Genomic DNA from Synechocystis sp. PCC 6803 This parts contains the coding region of Acetate kinase (AckA) + a potential native terminator (50 bp downstream of the gene) from Synechocystis sp. PCC 6803. It's involved in the Phosphate acetyltransferase-acetate kinase pathway and catalyzes the conversion of acetyl-CoA to acetate. In our project this part was overexpressed in Synechocystis to increase the acetate production. false false _2497_ 27749 27749 9 false The complete sequence of the genome of Synechocystis sp. PCC 6803(Genbank accession number NC_000911) was used to design primers for amplification of this gene. FW primer: ATGAAATTCCTGATTCTCAATGC RV primer: AGCCTAATTCAACATTTATCTTCAC The reverse primer was designed to also include 50 bp downstream of the gene as a potential native terminator. false John Hellgren annotation2481487 1 AckA range2481487 1 1 1242 BBa_K2030002_sequence 1 atgaaattcctgattctcaatgccggttccagcagtcaaaaaagttgtctttatgagctgactggcgatcgcctaccggagacgataccggagcccttatgggaggctttcattgattggacggtgttggcaaatcaggggcggttaacggtggaaactgctggccaaaaacaagtgatcattctggaaactggcgatcgccaacaaggtatagcccgaatgttggacaccctggtaacgggggatgatgcagtgttgaaaagtttggcagagattgacctggtgggccatcgagtggtccatggaggaacggatcatgccgaagccactttgatcacgccggaagtgcaacaggccattgcagacttaattcccctggctccagcccataatcctgctcacctagagggcattgaagcaatcagcgcactgttggtgttgggggaagtgccccagatcgctgtgttcgacacggcttttcaccgcaccattcccacccctgctgccgaatatcccattcctcaagcctggactaatttaggcatccgtcgctatgggttccacggcactagccacaaatattgtgcccagaaaactgcggaaattttaggtaaacctttggcggatttaaaactcatcacctgccacattggtaacggtgcctccctaacggcgattaaaaatggggtcagcattgataccactatgggcttcactcccctagaaggcttgatgatgggggcccggagcggttccattgacccggcaattctactttttttgcaagaaacccaagggttaaccccggcggaaattaacaccaccctcaataaaaaatccggtttgctcggagtctctgggctgtcggcggatcttcgtaccattttgcaggccaaagcagagggtaatgaacaagctcaattggcttatgtcatgtatatccatcgcttccggagttgtttggggcaaatgattgcttccttggaaggtttggatacgttggtgtttaccgccggggtgggggaaaatgccgccactgtgcgggcagatgtttgccaagcttttgaatttctaggtttaaaacttgatccagagttgaataaccgatcgccaagggatactgtcatttctcactccgactccttggtgacggtgttgattgtccacaccgaagaagattgggcgatcgcccaggattgttggcactggtggcatagccagggacagagaaagcaatcgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z