LOCUS BBa_K203099 5139 bp DNA linear 17-Dec-2024 DEFINITION pSMB_REFERENCE ACCESSION BBa_K203099 VERSION BBa_K203099.1 FEATURES Location/Qualifiers CDS 373..1083 /label=mcherry polyA_site 2815..2934 /label=SV40 terminator CDS 1672..2679 /label=Hygromycine Resistance misc_feature 1605..1652 /label=FRT site promoter 188..366 /label=JeT CDS 4143..5003 /label=Amp resistance promoter 5045..5073 /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattggtga gaattcgcgg 181 ccgcactagt gggcggagtt agggcggagc caatcagcgt gcgccgttcc gaaagttgcc 241 ttttatggct gggcggaagc ttagaatggg cggtgaacgc cgatgattat ataaggacgc 301 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt 361 gctagggcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 421 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 481 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 541 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 601 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 661 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 721 tcccttcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 781 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 841 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 901 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 961 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1021 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1081 taatgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 1141 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 1201 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 1261 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 1321 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc 1381 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 1441 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 1501 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 1561 ctcgacccca aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga 1621 agttcctatt ctctagaaag tataggaact tccttggcca aaaagcctga actcaccgcg 1681 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc 1741 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg 1801 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca 1861 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg agttcagcga gagcctgacc 1921 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg 1981 cccgctgttc tacagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc 2041 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt 2101 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac 2161 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc 2221 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat 2281 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag 2341 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac 2401 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc 2461 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg 2521 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa 2581 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt 2641 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc 2701 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 2761 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 2821 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 2881 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 2941 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 3001 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 3061 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 3121 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 3181 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 3241 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 3301 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 3361 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 3421 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 3481 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 3541 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 3601 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 3661 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 3721 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 3781 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 3841 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 3901 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 3961 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 4021 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 4081 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 4141 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 4201 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 4261 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 4321 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 4381 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 4441 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 4501 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 4561 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 4621 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 4681 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 4741 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 4801 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 4861 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 4921 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 4981 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 5041 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 5101 gttccgcgca catttccccg aaaagtgcca cctgacgtc //