##gff-version 3
##sequence-region BBa_K2032000 1 5403
BBa_K2032000	.	polypeptide_domain	3560	4729	.	+	0	ID=BBa_K1390005;Name=BBa_K1390005
BBa_K2032000	.	ribosome_entry_site	4738	4750	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	4738	4750	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K2032000	.	engineered_region	4738	4750	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	4744	4747	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	1930	1942	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	1930	1942	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K2032000	.	engineered_region	1930	1942	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	1936	1939	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2032000	.	polypeptide_domain	4757	5266	.	+	0	ID=BBa_K1390006;Name=BBa_K1390006
BBa_K2032000	.	polypeptide_domain	1949	3532	.	+	0	ID=BBa_K1390004;Name=BBa_K1390004
BBa_K2032000	.	terminator	5275	5403	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2032000	.	terminator	5363	5403	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2032000	.	stop_codon	5396	5396	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2032000	.	stem_loop	5370	5389	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2032000	.	engineered_region	5363	5403	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2032000	.	polyA_site	5390	5403	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2032000	.	terminator	5275	5354	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2032000	.	stem_loop	5286	5329	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2032000	.	engineered_region	5275	5354	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2032000	.	ribosome_entry_site	517	529	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	517	529	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K2032000	.	engineered_region	517	529	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	523	526	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	1591	1603	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	1591	1603	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K2032000	.	engineered_region	1591	1603	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	1597	1600	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2032000	.	polypeptide_domain	83	508	.	+	0	ID=BBa_K1390001;Name=BBa_K1390001
BBa_K2032000	.	polypeptide_domain	1610	1921	.	+	0	ID=BBa_K1390003;Name=BBa_K1390003
BBa_K2032000	.	polypeptide_domain	536	1582	.	+	0	ID=BBa_K1390002;Name=BBa_K1390002
BBa_K2032000	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K2032000	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	3541	3553	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	3541	3553	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K2032000	.	engineered_region	3541	3553	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2032000	.	ribosome_entry_site	3547	3550	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2032000	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K2032000	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K2032000	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K2032000	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K2032000	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K2032000	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
>BBa_K2032000
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtac
tagatgagcgtaaacagcaacgcatacgacgccggcatcatgggcctgaaaggcaaggacttcgccgatcagttctttg
ccgacgaaaaccaagtggtccatgaaagcgacacggtcgttctggtcctcaagaagtcggacgagatcaatacctttat
cgaggagatccttctgacggactacaagaagaacgtcaatccgacggtaaacgtggaagaccgcgcgggttactggtgg
atcaaggccaacggcaagatcgaggtcgattgcgacgagatttccgagctgttggggcggcagttcaacgtctacgact
tcctcgtcgacgtttcctccaccatcggccgggcctataccctgggcaacaagttcaccattaccagtgagctgatggg
cctggaccgcaagctcgaagactatcacgcttaatactagagtcacacaggaaagtactagatgcagcgagttcacact
atcacggcggtgacggaggatggcgaatcgctccgcttcgaatgccgttcggacgaggacgtcatcaccgccgccctgc
gccagaacatctttctgatgtcgtcctgccgggagggcggctgtgcgacctgcaaggccttgtgcagcgaaggggacta
cgacctcaagggctgtagcgttcaggcgctgccgccggaagaggaggaggaagggttggtgttgttgtgccggacctac
ccgaagaccgacctggaaatcgaactgccctatacccattgccgcatcagttttggtgaggtcggcagtttcgaggcgg
aggtcgtcggcctcaactgggtttcgagcaacaccgtccagtttcttttgcagaagcggcccgacgagtgcggcaaccg
tggcgtgaaattcgaacccggtcagttcatggacctgaccatccccggcaccgatgtctcccgctcctactcgccggcg
aaccttcctaatcccgaaggccgcctggagttcctgatccgcgtgttaccggagggacggttttcggactacctgcgca
atgacgcgcgtgtcggacaggtcctctcggtcaaagggccactgggcgtgttcggtctcaaggagcggggcatggcgcc
gcgctatttcgtggccggcggcaccgggttggcgccggtggtctcgatggtgcggcagatgcaggagtggaccgcgccg
aacgagacccgcatctatttcggtgtgaacaccgagccggaattgttctacatcgacgagctcaaatccctggaacgat
cgatgcgcaatctcaccgtgaaggcctgtgtctggcacccgagcggggactgggaaggcgagcagggctcgcccatcga
tgcgttgcgggaagacctggagtcctccgacgccaacccggacatttatttgtgcggtccgccgggcatgatcgatgcc
gcctgcgagctggtacgcagccgcggtatccccggcgaacaggtcttcttcgaaaaattcctgccgtccggggcggcct
gatactagagtcacacaggaaagtactagatggtcgaatcggcatttcagccattttcgggcgacgcagacgaatggtt
cgaggaaccacggccccaggccggtttcttcccttccgcggactggcatctgctcaaacgggacgagacctacgcagcc
tatgccaaggatctcgatttcatgtggcggtgggtcatcgtccgggaagaaaggatcgtccaggagggttgctcgatca
gcctggagtcgtcgatccgcgccgtgacgcacgtactgaattattttggtatgaccgaacaacgcgccccggcagagga
ccggaccggcggagttcaacattgatactagagtcacacaggaaagtactagatggcacttagcaccgcaaccaaggcc
gcgacggacgcgctggctgccaatcgggcacccaccagcgtgaatgcacaggaagtgcaccgttggctccagagcttca
actgggatttcaagaacaaccggaccaagtacgccaccaagtacaagatggcgaacgagaccaaggaacagttcaagct
gatcgccaaggaatatgcgcgcatggaggcagtcaaggacgaaaggcagttcggtagcctgcaagatgcgctgacccgc
ctcaacgccggtgttcgcgttcatccgaagtggaacgagaccatgaaagtggtttcgaacttcctggaagtgggcgaat
acaacgccatcgccgctaccgggatgctgtgggattccgcccaggcggcggaacagaagaacggctatctggcccaggt
gttggatgaaatccgccacacccaccagtgtgcctacgtcaactactacttcgcgaagaacggccaggacccggccggt
cacaacgatgctcgccgcacccgtaccatcggtccgctgtggaagggcatgaagcgcgtgttttccgacggcttcattt
ccggcgacgccgtggaatgctccctcaacctgcaactggtgggtgaggcctgcttcaccaatccgctgatcgtcgcagt
gaccgaatgggctgccgccaacggcgatgaaatcaccccgacggtgttcctgtcgatcgagaccgacgaactgcgccac
atggccaacggttaccagaccgtcgtttccatcgccaacgatccggcttccgccaagtatctcaacacggacctgaaca
acgccttctggacccagcagaagtacttcacgccggtgttgggcatgctgttcgagtatggctccaagttcaaggtcga
gccgtgggtcaagacgtggaaccgctgggtgtacgaggactggggcggcatctggatcggccgtctgggcaagtacggg
gtggagtcgccgcgcagcctcaaggacgccaagcaggacgcttactgggctcaccacgacctgtatctgctggcttatg
cgctgtggccgaccggcttcttccgtctggcgctgccggatcaggaagaaatggagtggttcgaggccaactaccccgg
ctggtacgaccactacggcaagatctacgaggaatggcgcgcccgcggttgcgaggatccgtcctcgggcttcatcccg
ctgatgtggttcatcgaaaacaaccatcccatctacatcgatcgcgtgtcgcaagtgccgttctgcccgagcttggcca
agggcgccagcaccctgcgcgtgcacgagtacaacggccagatgcacaccttcagcgaccagtggggcgagcgcatgtg
gctggccgagccggagcgctacgagtgccagaacatcttcgaacagtacgaaggacgcgaactgtcggaagtgatcgcc
gaactgcacgggctgcgcagtgatggcaagaccctgatcgcccagccgcatgtccgtggcgacaagctgtggacgttgg
acgatatcaaacgcctgaactgcgtcttcaagaacccggtgaaggcattcaattgatactagagtcacacaggaaagta
ctagatgagcatgttaggagaaagacgccgcggtctgaccgatccggaaatggcggccgtcattttgaaggcgcttcct
gaagctccgctggacggcaacaacaagatgggttatttcgtcaccccccgctggaaacgcttgacggaatatgaagccc
tgaccgtttatgcgcagcccaacgccgactggatcgccggcggcctggactggggcgactggacccagaaattccacgg
cggccgcccttcctggggcaacgagaccacggagctgcgcaccgtcgactggttcaagcaccgtgacccgctccgccgt
tggcatgcgccgtacgtcaaggacaaggccgaggaatggcgctacaccgaccgcttcctgcaaggttactccgccgacg
gtcagatccgggcgatgaacccgacctggcgggacgagttcatcaaccggtattggggcgccttcctgttcaacgaata
cggattgttcaacgctcattcgcagggcgcccgggaggcgctgtcggacgtaacccgcgtcagcctggctttctggggc
ttcgacaagatcgacatcgcccagatgatccaactcgaacggggtttcctcgccaagatcgtacccggtttcgacgagt
ccacagcggtgccgaaggccgaatggacgaacggggaggtctacaagagcgcccgtctggccgtggaagggctgtggca
ggaggtgttcgactggaacgagagcgctttctcggtgcacgccgtctatgacgcgctgttcggtcagttcgtccgccgc
gagttctttcagcggctggctccccgcttcggcgacaatctgacgccattcttcatcaaccaggcccagacatacttcc
agatcgccaagcagggcgtacaggatctgtattacaactgtctgggtgacgatccggagttcagcgattacaaccgtac
cgtgatgcgcaactggaccggcaagtggctggagcccacgatcgccgctctgcgcgacttcatggggctgtttgcgaag
ctgccggcgggcaccactgacaaggaagaaatcaccgcgtccctgtaccgggtggtcgacgactggatcgaggactacg
ccagcaggatcgacttcaaggcggaccgcgatcagatcgttaaagcggttctggcaggattgaaatgatactagagtca
cacaggaaagtactagatggcgaaactgggtatacacagcaacgacacccgcgacgcctgggtgaacaagatcgcgcag
ctcaacaccctggaaaaagcggccgagatgctgaagcagttccggatggaccacaccacgccgttccgcaacagctacg
aactggacaacgactacctctggatcgaggccaagctcgaagagaaggtcgccgtcctcaaggcacgcgccttcaacga
ggtggacttccgtcataagaccgctttcggcgaggatgccaagtccgttctggacggcaccgtcgcgaagatgaacgcg
gccaaggacaagtgggaggcggagaagatccatatcggtttccgccaggcctacaagccgccgatcatgccggtgaact
atttcctggacggcgagcgtcagttggggacccggctgatggaactgcgcaacctcaactactacgacacgccgctgga
agaactgcgcaaacagcgcggtgtgcgggtggtgcatctgcaatcgccgtgatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata