BBa_K2032000
type: ComponentDefinition
persistent identity: BBa K2032000
display id: BBa_K2032000
version: 1
was generated by: iGEM to SBOL conversion
description: sMMO
created: 2016-08-26T11:00:00Z
modified: 2016-08-27T01:05:40Z
mutable provenance: Methylococcus capsulatus, a methanotrophic bacteria
owned by: james
owned by: myers
mutable description: Contains the subunits necessary to assembly the soluble methane monooxygenase (sMMO) enzyme complex. Contains MMO subunits B, C, D, X, Y, and Z, each preceded by Anderson rbs B0032, and all under lactose of IPTG inducable control. The sMMO complex oxidizes methane into methanol. (add more detail later)
discontinued: false
dominant: false
experience: experience/None
group u list: _2499_
m user id: 26329
owner id: 26329
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: A second terminator and promoter to divide the transcript into was considered to avoid potential complications with a long mRNA.
star: false
creator: Jacob Premo, Seth Cohen, Chun Mun Loke
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1390003
component: BBa_B0032
component: BBa_K1390004
component: BBa_K1390005
component: BBa_K1390006
component: BBa_R0011
component: BBa_K1390001
component: BBa_K1390002
sequence annotation: BBa_R0011
sequence annotation: BBa_B0032
sequence annotation: BBa_K1390001
sequence annotation: BBa_K1390002
sequence annotation: BBa_K1390003
sequence annotation: BBa_K1390004
sequence annotation: BBa_K1390005
sequence annotation: BBa_K1390006
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K2032000_sequence/1

BBa_B0015
type: Component
persistent identity: component2481797
display id: component2481797
version: 1
top level: BBa_K2032000
definition: BBa_B0015
access: public

BBa_K1390003
type: Component
persistent identity: component2481778
display id: component2481778
version: 1
top level: BBa_K2032000
definition: BBa_K1390003
access: public

BBa_B0032
type: Component
persistent identity: component2481776
display id: component2481776
version: 1
top level: BBa_K2032000
definition: BBa_B0032
access: public

BBa_K1390004
type: Component
persistent identity: component2481782
display id: component2481782
version: 1
top level: BBa_K2032000
definition: BBa_K1390004
access: public

BBa_B0032
type: Component
persistent identity: component2481780
display id: component2481780
version: 1
top level: BBa_K2032000
definition: BBa_B0032
access: public

BBa_K1390005
type: Component
persistent identity: component2481786
display id: component2481786
version: 1
top level: BBa_K2032000
definition: BBa_K1390005
access: public

BBa_B0032
type: Component
persistent identity: component2481784
display id: component2481784
version: 1
top level: BBa_K2032000
definition: BBa_B0032
access: public

BBa_K1390006
type: Component
persistent identity: component2481790
display id: component2481790
version: 1
top level: BBa_K2032000
definition: BBa_K1390006
access: public

BBa_B0032
type: Component
persistent identity: component2481788
display id: component2481788
version: 1
top level: BBa_K2032000
definition: BBa_B0032
access: public

BBa_R0011
type: Component
persistent identity: component2481762
display id: component2481762
version: 1
top level: BBa_K2032000
definition: lacI+pL
access: public

BBa_K1390001
type: Component
persistent identity: component2481770
display id: component2481770
version: 1
top level: BBa_K2032000
definition: BBa_K1390001
access: public

BBa_B0032
type: Component
persistent identity: component2481768
display id: component2481768
version: 1
top level: BBa_K2032000
definition: BBa_B0032
access: public

BBa_K1390002
type: Component
persistent identity: component2481774
display id: component2481774
version: 1
top level: BBa_K2032000
definition: BBa_K1390002
access: public

BBa_B0032
type: Component
persistent identity: component2481772
display id: component2481772
version: 1
top level: BBa_K2032000
definition: BBa_B0032
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2481762
display id: annotation2481762
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481762/range2481762/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481762/range2481762/1
type: Range
persistent identity: range2481762
display id: range2481762
version: 1
top level: BBa_K2032000
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2481768
display id: annotation2481768
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481768/range2481768/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481768/range2481768/1
type: Range
persistent identity: range2481768
display id: range2481768
version: 1
top level: BBa_K2032000
direction: direction/forward
start: 64
end: 76
orientation: inline

BBa_K1390001
type: SequenceAnnotation
persistent identity: annotation2481770
display id: annotation2481770
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481770/range2481770/1
component: BBa_K1390001

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481770/range2481770/1
type: Range
persistent identity: range2481770
display id: range2481770
version: 1
top level: BBa_K2032000
direction: direction/reverse
start: 83
end: 508
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2481772
display id: annotation2481772
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481772/range2481772/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481772/range2481772/1
type: Range
persistent identity: range2481772
display id: range2481772
version: 1
top level: BBa_K2032000
direction: direction/forward
start: 517
end: 529
orientation: inline

BBa_K1390002
type: SequenceAnnotation
persistent identity: annotation2481774
display id: annotation2481774
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481774/range2481774/1
component: BBa_K1390002

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481774/range2481774/1
type: Range
persistent identity: range2481774
display id: range2481774
version: 1
top level: BBa_K2032000
direction: direction/reverse
start: 536
end: 1582
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2481776
display id: annotation2481776
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481776/range2481776/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481776/range2481776/1
type: Range
persistent identity: range2481776
display id: range2481776
version: 1
top level: BBa_K2032000
direction: direction/forward
start: 1591
end: 1603
orientation: inline

BBa_K1390003
type: SequenceAnnotation
persistent identity: annotation2481778
display id: annotation2481778
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481778/range2481778/1
component: BBa_K1390003

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481778/range2481778/1
type: Range
persistent identity: range2481778
display id: range2481778
version: 1
top level: BBa_K2032000
direction: direction/reverse
start: 1610
end: 1921
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2481780
display id: annotation2481780
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481780/range2481780/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481780/range2481780/1
type: Range
persistent identity: range2481780
display id: range2481780
version: 1
top level: BBa_K2032000
direction: direction/forward
start: 1930
end: 1942
orientation: inline

BBa_K1390004
type: SequenceAnnotation
persistent identity: annotation2481782
display id: annotation2481782
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481782/range2481782/1
component: BBa_K1390004

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481782/range2481782/1
type: Range
persistent identity: range2481782
display id: range2481782
version: 1
top level: BBa_K2032000
direction: direction/reverse
start: 1949
end: 3532
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2481784
display id: annotation2481784
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481784/range2481784/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481784/range2481784/1
type: Range
persistent identity: range2481784
display id: range2481784
version: 1
top level: BBa_K2032000
direction: direction/forward
start: 3541
end: 3553
orientation: inline

BBa_K1390005
type: SequenceAnnotation
persistent identity: annotation2481786
display id: annotation2481786
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481786/range2481786/1
component: BBa_K1390005

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481786/range2481786/1
type: Range
persistent identity: range2481786
display id: range2481786
version: 1
top level: BBa_K2032000
direction: direction/reverse
start: 3560
end: 4729
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2481788
display id: annotation2481788
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481788/range2481788/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481788/range2481788/1
type: Range
persistent identity: range2481788
display id: range2481788
version: 1
top level: BBa_K2032000
direction: direction/forward
start: 4738
end: 4750
orientation: inline

BBa_K1390006
type: SequenceAnnotation
persistent identity: annotation2481790
display id: annotation2481790
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481790/range2481790/1
component: BBa_K1390006

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481790/range2481790/1
type: Range
persistent identity: range2481790
display id: range2481790
version: 1
top level: BBa_K2032000
direction: direction/reverse
start: 4757
end: 5266
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2481797
display id: annotation2481797
version: 1
top level: BBa_K2032000
location: https://synbiohub.org/public/igem/BBa_K2032000/annotation2481797/range2481797/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2032000/annotation2481797/range2481797/1
type: Range
persistent identity: range2481797
display id: range2481797
version: 1
top level: BBa_K2032000
direction: direction/reverse
start: 5275
end: 5403
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K1390001
type: ComponentDefinition
persistent identity: BBa K1390001
display id: BBa_K1390001
version: 1
was generated by: iGEM to SBOL conversion
description: mmoB
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:13Z
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath
owned by: james
owned by: myers
mutable description: mmoB is one subunit of the mmo from Methylococcus capsulatus bath
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: the brick is constructed in the RCF10 standard
star: false
creator: Stefan D??bel
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K1390001_sequence/1

BBa_K1390002
type: ComponentDefinition
persistent identity: BBa K1390002
display id: BBa_K1390002
version: 1
was generated by: iGEM to SBOL conversion
description: mmoC
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:13Z
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath
owned by: james
owned by: myers
mutable description: mmoC is one subunit of the mmo from Methylococcus capsulatus bath
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: the brick is constructed in the RCF10 standard
star: false
creator: Stefan D??bel
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K1390002_sequence/1

BBa_K1390003
type: ComponentDefinition
persistent identity: BBa K1390003
display id: BBa_K1390003
version: 1
was generated by: iGEM to SBOL conversion
description: mmoD
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:14Z
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath
owned by: james
owned by: myers
mutable description: mmoD is one subunit of the mmo from Methylococcus capsulatus bath
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: the brick is constructed in the RCF10 standard
star: false
creator: Stefan D??bel
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1390003_sequence/1

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1390004
type: ComponentDefinition
persistent identity: BBa K1390004
display id: BBa_K1390004
version: 1
was generated by: iGEM to SBOL conversion
description: mmoX
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:14Z
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath without any PstI restriction site
owned by: james
owned by: myers
mutable description: mmoX is one subunit of the mmo from Methylococcus capsulatus bath
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: the brick is constructed in the RCF10 standard
star: false
creator: Stefan D??bel
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K1390004_sequence/1

BBa_K1390005
type: ComponentDefinition
persistent identity: BBa K1390005
display id: BBa_K1390005
version: 1
was generated by: iGEM to SBOL conversion
description: mmoY
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:14Z
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath without any PstI restriction site
owned by: james
owned by: myers
mutable description: mmoY is one subunit of the mmo from Methylococcus capsulatus bath
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: the brick is constructed in the RCF10 standard
star: false
creator: Stefan D??bel
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1390005_sequence/1

BBa_K1390006
type: ComponentDefinition
persistent identity: BBa K1390006
display id: BBa_K1390006
version: 1
was generated by: iGEM to SBOL conversion
description: mmoZ
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:14Z
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath
owned by: james
owned by: myers
mutable description: mmoZ is one subunit of the mmo from Methylococcus capsulatus bath
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: the brick is constructed in the RCF10 standard
star: false
creator: Stefan D??bel
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1390006_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K2032000_sequence/1
type: Sequence
persistent identity: BBa K2032000 sequence
display id: BBa_K2032000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtactagatgagcgtaaacagcaacgcatacgacgccggcatcatgggcctgaaaggcaaggacttcgccgatcagttctttgccgacgaaaaccaagtggtccatgaaagcgacacggtcgttctggtcctcaagaagtcggacgagatcaatacctttatcgaggagatccttctgacggactacaagaagaacgtcaatccgacggtaaacgtggaagaccgcgcgggttactggtggatcaaggccaacggcaagatcgaggtcgattgcgacgagatttccgagctgttggggcggcagttcaacgtctacgacttcctcgtcgacgtttcctccaccatcggccgggcctataccctgggcaacaagttcaccattaccagtgagctgatgggcctggaccgcaagctcgaagactatcacgcttaatactagagtcacacaggaaagtactagatgcagcgagttcacactatcacggcggtgacggaggatggcgaatcgctccgcttcgaatgccgttcggacgaggacgtcatcaccgccgccctgcgccagaacatctttctgatgtcgtcctgccgggagggcggctgtgcgacctgcaaggccttgtgcagcgaaggggactacgacctcaagggctgtagcgttcaggcgctgccgccggaagaggaggaggaagggttggtgttgttgtgccggacctacccgaagaccgacctggaaatcgaactgccctatacccattgccgcatcagttttggtgaggtcggcagtttcgaggcggaggtcgtcggcctcaactgggtttcgagcaacaccgtccagtttcttttgcagaagcggcccgacgagtgcggcaaccgtggcgtgaaattcgaacccggtcagttcatggacctgaccatccccggcaccgatgtctcccgctcctactcgccggcgaaccttcctaatcccgaaggccgcctggagttcctgatccgcgtgttaccggagggacggttttcggactacctgcgcaatgacgcgcgtgtcggacaggtcctctcggtcaaagggccactgggcgtgttcggtctcaaggagcggggcatggcgccgcgctatttcgtggccggcggcaccgggttggcgccggtggtctcgatggtgcggcagatgcaggagtggaccgcgccgaacgagacccgcatctatttcggtgtgaacaccgagccggaattgttctacatcgacgagctcaaatccctggaacgatcgatgcgcaatctcaccgtgaaggcctgtgtctggcacccgagcggggactgggaaggcgagcagggctcgcccatcgatgcgttgcgggaagacctggagtcctccgacgccaacccggacatttatttgtgcggtccgccgggcatgatcgatgccgcctgcgagctggtacgcagccgcggtatccccggcgaacaggtcttcttcgaaaaattcctgccgtccggggcggcctgatactagagtcacacaggaaagtactagatggtcgaatcggcatttcagccattttcgggcgacgcagacgaatggttcgaggaaccacggccccaggccggtttcttcccttccgcggactggcatctgctcaaacgggacgagacctacgcagcctatgccaaggatctcgatttcatgtggcggtgggtcatcgtccgggaagaaaggatcgtccaggagggttgctcgatcagcctggagtcgtcgatccgcgccgtgacgcacgtactgaattattttggtatgaccgaacaacgcgccccggcagaggaccggaccggcggagttcaacattgatactagagtcacacaggaaagtactagatggcacttagcaccgcaaccaaggccgcgacggacgcgctggctgccaatc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1390001_sequence/1
type: Sequence
persistent identity: BBa K1390001 sequence
display id: BBa_K1390001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcgtaaacagcaacgcatacgacgccggcatcatgggcctgaaaggcaaggacttcgccgatcagttctttgccgacgaaaaccaagtggtccatgaaagcgacacggtcgttctggtcctcaagaagtcggacgagatcaatacctttatcgaggagatccttctgacggactacaagaagaacgtcaatccgacggtaaacgtggaagaccgcgcgggttactggtggatcaaggccaacggcaagatcgaggtcgattgcgacgagatttccgagctgttggggcggcagttcaacgtctacgacttcctcgtcgacgtttcctccaccatcggccgggcctataccctgggcaacaagttcaccattaccagtgagctgatgggcctggaccgcaagctcgaagactatcacgcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1390002_sequence/1
type: Sequence
persistent identity: BBa K1390002 sequence
display id: BBa_K1390002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagcgagttcacactatcacggcggtgacggaggatggcgaatcgctccgcttcgaatgccgttcggacgaggacgtcatcaccgccgccctgcgccagaacatctttctgatgtcgtcctgccgggagggcggctgtgcgacctgcaaggccttgtgcagcgaaggggactacgacctcaagggctgtagcgttcaggcgctgccgccggaagaggaggaggaagggttggtgttgttgtgccggacctacccgaagaccgacctggaaatcgaactgccctatacccattgccgcatcagttttggtgaggtcggcagtttcgaggcggaggtcgtcggcctcaactgggtttcgagcaacaccgtccagtttcttttgcagaagcggcccgacgagtgcggcaaccgtggcgtgaaattcgaacccggtcagttcatggacctgaccatccccggcaccgatgtctcccgctcctactcgccggcgaaccttcctaatcccgaaggccgcctggagttcctgatccgcgtgttaccggagggacggttttcggactacctgcgcaatgacgcgcgtgtcggacaggtcctctcggtcaaagggccactgggcgtgttcggtctcaaggagcggggcatggcgccgcgctatttcgtggccggcggcaccgggttggcgccggtggtctcgatggtgcggcagatgcaggagtggaccgcgccgaacgagacccgcatctatttcggtgtgaacaccgagccggaattgttctacatcgacgagctcaaatccctggaacgatcgatgcgcaatctcaccgtgaaggcctgtgtctggcacccgagcggggactgggaaggcgagcagggctcgcccatcgatgcgttgcgggaagacctggagtcctccgacgccaacccggacatttatttgtgcggtccgccgggcatgatcgatgccgcctgcgagctggtacgcagccgcggtatccccggcgaacaggtcttcttcgaaaaattcctgccgtccggggcggcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1390003_sequence/1
type: Sequence
persistent identity: BBa K1390003 sequence
display id: BBa_K1390003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtcgaatcggcatttcagccattttcgggcgacgcagacgaatggttcgaggaaccacggccccaggccggtttcttcccttccgcggactggcatctgctcaaacgggacgagacctacgcagcctatgccaaggatctcgatttcatgtggcggtgggtcatcgtccgggaagaaaggatcgtccaggagggttgctcgatcagcctggagtcgtcgatccgcgccgtgacgcacgtactgaattattttggtatgaccgaacaacgcgccccggcagaggaccggaccggcggagttcaacattga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1390004_sequence/1
type: Sequence
persistent identity: BBa K1390004 sequence
display id: BBa_K1390004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcacttagcaccgcaaccaaggccgcgacggacgcgctggctgccaatcgggcacccaccagcgtgaatgcacaggaagtgcaccgttggctccagagcttcaactgggatttcaagaacaaccggaccaagtacgccaccaagtacaagatggcgaacgagaccaaggaacagttcaagctgatcgccaaggaatatgcgcgcatggaggcagtcaaggacgaaaggcagttcggtagcctgcaagatgcgctgacccgcctcaacgccggtgttcgcgttcatccgaagtggaacgagaccatgaaagtggtttcgaacttcctggaagtgggcgaatacaacgccatcgccgctaccgggatgctgtgggattccgcccaggcggcggaacagaagaacggctatctggcccaggtgttggatgaaatccgccacacccaccagtgtgcctacgtcaactactacttcgcgaagaacggccaggacccggccggtcacaacgatgctcgccgcacccgtaccatcggtccgctgtggaagggcatgaagcgcgtgttttccgacggcttcatttccggcgacgccgtggaatgctccctcaacctgcaactggtgggtgaggcctgcttcaccaatccgctgatcgtcgcagtgaccgaatgggctgccgccaacggcgatgaaatcaccccgacggtgttcctgtcgatcgagaccgacgaactgcgccacatggccaacggttaccagaccgtcgtttccatcgccaacgatccggcttccgccaagtatctcaacacggacctgaacaacgccttctggacccagcagaagtacttcacgccggtgttgggcatgctgttcgagtatggctccaagttcaaggtcgagccgtgggtcaagacgtggaaccgctgggtgtacgaggactggggcggcatctggatcggccgtctgggcaagtacggggtggagtcgccgcgcagcctcaaggacgccaagcaggacgcttactgggctcaccacgacctgtatctgctggcttatgcgctgtggccgaccggcttcttccgtctggcgctgccggatcaggaagaaatggagtggttcgaggccaactaccccggctggtacgaccactacggcaagatctacgaggaatggcgcgcccgcggttgcgaggatccgtcctcgggcttcatcccgctgatgtggttcatcgaaaacaaccatcccatctacatcgatcgcgtgtcgcaagtgccgttctgcccgagcttggccaagggcgccagcaccctgcgcgtgcacgagtacaacggccagatgcacaccttcagcgaccagtggggcgagcgcatgtggctggccgagccggagcgctacgagtgccagaacatcttcgaacagtacgaaggacgcgaactgtcggaagtgatcgccgaactgcacgggctgcgcagtgatggcaagaccctgatcgcccagccgcatgtccgtggcgacaagctgtggacgttggacgatatcaaacgcctgaactgcgtcttcaagaacccggtgaaggcattcaattga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1390005_sequence/1
type: Sequence
persistent identity: BBa K1390005 sequence
display id: BBa_K1390005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcatgttaggagaaagacgccgcggtctgaccgatccggaaatggcggccgtcattttgaaggcgcttcctgaagctccgctggacggcaacaacaagatgggttatttcgtcaccccccgctggaaacgcttgacggaatatgaagccctgaccgtttatgcgcagcccaacgccgactggatcgccggcggcctggactggggcgactggacccagaaattccacggcggccgcccttcctggggcaacgagaccacggagctgcgcaccgtcgactggttcaagcaccgtgacccgctccgccgttggcatgcgccgtacgtcaaggacaaggccgaggaatggcgctacaccgaccgcttcctgcaaggttactccgccgacggtcagatccgggcgatgaacccgacctggcgggacgagttcatcaaccggtattggggcgccttcctgttcaacgaatacggattgttcaacgctcattcgcagggcgcccgggaggcgctgtcggacgtaacccgcgtcagcctggctttctggggcttcgacaagatcgacatcgcccagatgatccaactcgaacggggtttcctcgccaagatcgtacccggtttcgacgagtccacagcggtgccgaaggccgaatggacgaacggggaggtctacaagagcgcccgtctggccgtggaagggctgtggcaggaggtgttcgactggaacgagagcgctttctcggtgcacgccgtctatgacgcgctgttcggtcagttcgtccgccgcgagttctttcagcggctggctccccgcttcggcgacaatctgacgccattcttcatcaaccaggcccagacatacttccagatcgccaagcagggcgtacaggatctgtattacaactgtctgggtgacgatccggagttcagcgattacaaccgtaccgtgatgcgcaactggaccggcaagtggctggagcccacgatcgccgctctgcgcgacttcatggggctgtttgcgaagctgccggcgggcaccactgacaaggaagaaatcaccgcgtccctgtaccgggtggtcgacgactggatcgaggactacgccagcaggatcgacttcaaggcggaccgcgatcagatcgttaaagcggttctggcaggattgaaatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1390006_sequence/1
type: Sequence
persistent identity: BBa K1390006 sequence
display id: BBa_K1390006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgaaactgggtatacacagcaacgacacccgcgacgcctgggtgaacaagatcgcgcagctcaacaccctggaaaaagcggccgagatgctgaagcagttccggatggaccacaccacgccgttccgcaacagctacgaactggacaacgactacctctggatcgaggccaagctcgaagagaaggtcgccgtcctcaaggcacgcgccttcaacgaggtggacttccgtcataagaccgctttcggcgaggatgccaagtccgttctggacggcaccgtcgcgaagatgaacgcggccaaggacaagtgggaggcggagaagatccatatcggtttccgccaggcctacaagccgccgatcatgccggtgaactatttcctggacggcgagcgtcagttggggacccggctgatggaactgcgcaacctcaactactacgacacgccgctggaagaactgcgcaaacagcgcggtgtgcgggtggtgcatctgcaatcgccgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z