LOCUS       BBa_K2032001            2654 bp    DNA     linear       26-Nov-2024
DEFINITION  Fructose Pathway
ACCESSION   BBa_K2032001
VERSION     BBa_K2032001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..54
                     /label=BBa_R0011
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     RBS             64..76
                     /label=RBS-3\Weak
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             70..73
                     /label=RBS
     promoter        91..146
                     /label=BBa_R0011
     RBS             153..166
                     /label=BBa_B0032
     CDS             172..1331
                     /label=MDH2
     misc_feature    85..1331
                     /label=BBa_K2032003
     RBS             1340..1352
                     /label=RBS-3\Weak
     misc_feature    1340..1352
                     /label=BBa_B0032
     RBS             1346..1349
                     /label=RBS
     CDS             1359..1997
                     /label=hps
     misc_feature    1359..1997
                     /label=BBa_K1585201
     RBS             2006..2018
                     /label=RBS-3\Weak
     misc_feature    2006..2018
                     /label=BBa_B0032
     RBS             2012..2015
                     /label=RBS
     CDS             2025..2582
                     /label=phi
     misc_feature    2025..2582
                     /label=BBa_K1585202
     misc_feature    2591..2670
                     /label=BBa_B0010
     stem_loop       2602..2645
                     /label=stem_loop
     misc_feature    2679..2719
                     /label=BBa_B0012
     stem_loop       2686..2705
                     /label=T7 TE
     polyA_site      2706..2719
                     /label=polya
     misc_feature    2712..2712
                     /label=stop
     misc_feature    2591..2719
                     /label=BBa_B0015
ORIGIN
        1 aagcttaatt gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac
       61 atacgtatca cacaggaaag aggcctatga caaacacaca atctgctttt ttcatgccat
      121 cggtgaatct gtttggggct ggaagcgtca atgaggtagg cacgcgtctg gcagacctgg
      181 gtgtcaaaaa ggcattatta gttactgatg caggtttaca cggattgggt ctttccgaaa
      241 agatttcaag cattattcgc gcagcgggcg tagaagtgtc gattttcccc aaagcagagc
      301 ctaatcccac agataaaaat gttgcagagg gcttagaggc gtataacgcg gaaaactgtg
      361 actccattgt cacgctgggt ggcggttcct cccatgatgc ggggaaagcg attgctcttg
      421 ttgcggctaa cgggggtaag attcatgact acgaaggggt agacgtttcg aaagagccga
      481 tggttccctt gatcgcgatc aataccactg ccggaacggg tagcgagctg acaaagttca
      541 cgattattac tgatacggaa cgcaaagtca aaatggcaat cgtagataaa catgttactc
      601 caaccctttc gatcaatgat ccagagctta tggttgggat gccaccctcg ctgacggccg
      661 cgactggctt ggacgcactt actcatgcga tcgaggcata cgtttcaacc ggggcgactc
      721 ctatcactga cgcgttggca atccaggcga tcaaaatcat cagtaagtac ttaccacgtg
      781 ctgttgcgaa tggtaaagac attgaggccc gtgaacaaat ggcgttcgct caatcacttg
      841 ctggcatggc ctttaacaac gcaggtttag gatacgtgca tgcaatcgcc catcagttgg
      901 ggggatttta caattttccg catggagtgt gcaacgctgt acttcttccc tacgtttgtc
      961 gctttaattt aatctctaaa gtagaacgct atgctgaaat cgcggcgttc ttgggagaaa
     1021 acgtggacgg cctgagtaca tatgatgctg ctgagaaggc cattaaggct atcgaacgta
     1081 tggccaagga tcttaacatc ccgaaggggt tcaaagaact gggagccaaa gaggaagata
     1141 tcgagacttt agccaagaac gcgatgaagg atgcttgtgc tctgacaaac cctcgcaagc
     1201 ccaagttgga ggaggtcatt cagatcatca aaaacgctat gtgataagtg gagatacata
     1261 cggggatcct gatctgtcac acaggaaaga ccggtatgga gttacaatta gctttagatt
     1321 tggttaacat cgaagaggct aaacaagtgg tggctgaagt ccaggagtac gtcgatattg
     1381 ttgaaatcgg gacccccgtc atcaagatct ggggcttaca ggccgttaaa gccgtgaagg
     1441 atgccttccc acatctgcaa gtcttagccg acatgaagac catggatgct gccgcatacg
     1501 aggtggcgaa ggccgcggaa catggcgcag atattgttac gatccttgca gcggcagagg
     1561 atgtatcgat taagggcgcc gtggaagaag caaaaaagtt agggaaaaaa atcctggtgg
     1621 acatgatcgc tgtaaaaaac ttagaagaac gtgctaagca agttgatgag atgggcgttg
     1681 actatatctg cgtgcatgca gggtatgact tgcaggctgt tggcaagaac cctttagatg
     1741 acctgaagcg cattaaggcg gtggtgaaga acgccaagac agccatcgcc ggtggaatca
     1801 agttagagac tttaccagag gttatcaagg ctgaacctga cttggtcatt gtagggggtg
     1861 gcatcgctaa tcagaccgac aagaaggcag ccgctgagaa gattaacaaa ctggtcaagc
     1921 aagggttgta ataaaatatt tcacacagga aaggccggca tgcttaccac cgagttctta
     1981 gccgaaatcg taaaagagct taacagctca gttaatcaga ttgcggatga ggaagcagag
     2041 gcacttgtta atggtatttt gcagagcaaa aaagttttcg ttgccggggc tggacgttct
     2101 ggcttcatgg caaagagttt tgctatgcgt atgatgcata tgggaatcga cgcttatgta
     2161 gtgggagaaa cagttacccc gaattacgaa aaagaagata tccttatcat tggatcggga
     2221 agtggggaaa cgaaatcgct tgtaagtatg gcgcagaaag ctaagagtat cgggggtact
     2281 attgccgctg tcacgatcaa cccagagtct acgattgggc aattagcgga tatcgtcatt
     2341 aaaatgcctg ggagccctaa agataaatca gaagcgcgcg agacgattca accaatgggc
     2401 tccttatttg agcagacatt attattgttt tatgacgccg tgattctgcg cttcatggaa
     2461 aagaagggct tagatacgaa gaccatgtat ggccgccacg caaaccttga ataataatac
     2521 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     2581 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     2641 tttctgcgtt tata
//