LOCUS BBa_K2032006 3731 bp DNA linear 22-Nov-2024 DEFINITION Fructose with RFP ACCESSION BBa_K2032006 VERSION BBa_K2032006.1 FEATURES Location/Qualifiers misc_feature 1..54 /label=BBa_R0011 misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 RBS 64..76 /label=RBS-3\Weak misc_feature 64..76 /label=BBa_B0032 RBS 70..73 /label=RBS promoter 91..146 /label=BBa_R0011 RBS 153..166 /label=BBa_B0032 CDS 172..1331 /label=MDH2 misc_feature 85..1331 /label=BBa_K2032003 RBS 1340..1352 /label=RBS-3\Weak misc_feature 1340..1352 /label=BBa_B0032 RBS 1346..1349 /label=RBS CDS 1359..1997 /label=hps misc_feature 1359..1997 /label=BBa_K1585201 RBS 2006..2018 /label=RBS-3\Weak misc_feature 2006..2018 /label=BBa_B0032 RBS 2012..2015 /label=RBS CDS 2025..2582 /label=phi misc_feature 2025..2582 /label=BBa_K1585202 misc_feature 2591..2670 /label=BBa_B0010 stem_loop 2602..2645 /label=stem_loop misc_feature 2679..2719 /label=BBa_B0012 stem_loop 2686..2705 /label=T7 TE polyA_site 2706..2719 /label=polya misc_feature 2712..2712 /label=stop misc_feature 2591..2719 /label=BBa_B0015 misc_feature 1..2654 /label=BBa_K2032001 promoter 2663..2862 /label=Promoter (lacI regulated) BBa_R0010 RBS 2871..2882 /label=RBS (Elowitz 1999) BBa_B0034 CDS 2889..3564 /label=Red Fluorescent Protein from Discosoma striata BBa_E1010 misc_feature 3570..3594 /label=Barcode misc_feature 3603..3731 /label=Double Terminator BBa_B0015 misc_feature 2663..3731 /label=BBa_K801100 ORIGIN 1 aagcttaatt gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac 61 atacgtatca cacaggaaag aggcctatga caaacacaca atctgctttt ttcatgccat 121 cggtgaatct gtttggggct ggaagcgtca atgaggtagg cacgcgtctg gcagacctgg 181 gtgtcaaaaa ggcattatta gttactgatg caggtttaca cggattgggt ctttccgaaa 241 agatttcaag cattattcgc gcagcgggcg tagaagtgtc gattttcccc aaagcagagc 301 ctaatcccac agataaaaat gttgcagagg gcttagaggc gtataacgcg gaaaactgtg 361 actccattgt cacgctgggt ggcggttcct cccatgatgc ggggaaagcg attgctcttg 421 ttgcggctaa cgggggtaag attcatgact acgaaggggt agacgtttcg aaagagccga 481 tggttccctt gatcgcgatc aataccactg ccggaacggg tagcgagctg acaaagttca 541 cgattattac tgatacggaa cgcaaagtca aaatggcaat cgtagataaa catgttactc 601 caaccctttc gatcaatgat ccagagctta tggttgggat gccaccctcg ctgacggccg 661 cgactggctt ggacgcactt actcatgcga tcgaggcata cgtttcaacc ggggcgactc 721 ctatcactga cgcgttggca atccaggcga tcaaaatcat cagtaagtac ttaccacgtg 781 ctgttgcgaa tggtaaagac attgaggccc gtgaacaaat ggcgttcgct caatcacttg 841 ctggcatggc ctttaacaac gcaggtttag gatacgtgca tgcaatcgcc catcagttgg 901 ggggatttta caattttccg catggagtgt gcaacgctgt acttcttccc tacgtttgtc 961 gctttaattt aatctctaaa gtagaacgct atgctgaaat cgcggcgttc ttgggagaaa 1021 acgtggacgg cctgagtaca tatgatgctg ctgagaaggc cattaaggct atcgaacgta 1081 tggccaagga tcttaacatc ccgaaggggt tcaaagaact gggagccaaa gaggaagata 1141 tcgagacttt agccaagaac gcgatgaagg atgcttgtgc tctgacaaac cctcgcaagc 1201 ccaagttgga ggaggtcatt cagatcatca aaaacgctat gtgataagtg gagatacata 1261 cggggatcct gatctgtcac acaggaaaga ccggtatgga gttacaatta gctttagatt 1321 tggttaacat cgaagaggct aaacaagtgg tggctgaagt ccaggagtac gtcgatattg 1381 ttgaaatcgg gacccccgtc atcaagatct ggggcttaca ggccgttaaa gccgtgaagg 1441 atgccttccc acatctgcaa gtcttagccg acatgaagac catggatgct gccgcatacg 1501 aggtggcgaa ggccgcggaa catggcgcag atattgttac gatccttgca gcggcagagg 1561 atgtatcgat taagggcgcc gtggaagaag caaaaaagtt agggaaaaaa atcctggtgg 1621 acatgatcgc tgtaaaaaac ttagaagaac gtgctaagca agttgatgag atgggcgttg 1681 actatatctg cgtgcatgca gggtatgact tgcaggctgt tggcaagaac cctttagatg 1741 acctgaagcg cattaaggcg gtggtgaaga acgccaagac agccatcgcc ggtggaatca 1801 agttagagac tttaccagag gttatcaagg ctgaacctga cttggtcatt gtagggggtg 1861 gcatcgctaa tcagaccgac aagaaggcag ccgctgagaa gattaacaaa ctggtcaagc 1921 aagggttgta ataaaatatt tcacacagga aaggccggca tgcttaccac cgagttctta 1981 gccgaaatcg taaaagagct taacagctca gttaatcaga ttgcggatga ggaagcagag 2041 gcacttgtta atggtatttt gcagagcaaa aaagttttcg ttgccggggc tggacgttct 2101 ggcttcatgg caaagagttt tgctatgcgt atgatgcata tgggaatcga cgcttatgta 2161 gtgggagaaa cagttacccc gaattacgaa aaagaagata tccttatcat tggatcggga 2221 agtggggaaa cgaaatcgct tgtaagtatg gcgcagaaag ctaagagtat cgggggtact 2281 attgccgctg tcacgatcaa cccagagtct acgattgggc aattagcgga tatcgtcatt 2341 aaaatgcctg ggagccctaa agataaatca gaagcgcgcg agacgattca accaatgggc 2401 tccttatttg agcagacatt attattgttt tatgacgccg tgattctgcg cttcatggaa 2461 aagaagggct tagatacgaa gaccatgtat ggccgccacg caaaccttga ataataatac 2521 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 2581 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 2641 tttctgcgtt tatatactag agcaatacgc aaaccgcctc tccccgcgcg ttggccgatt 2701 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 2761 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 2821 cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag aaagaggaga 2881 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta 2941 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt 3001 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt 3061 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg 3121 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga 3181 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt 3241 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga 3301 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga 3361 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta 3421 aaaccaccta catggctaaa aagccggttc agctgccggg tgcttacaaa accgacatca 3481 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg 3541 aaggtcgtca ctccaccggc gcttaataac gctgatagtg ctagtgtaga tcgctactag 3601 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 3661 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 3721 ctgcgtttat a //