##gff-version 3
##sequence-region BBa_K2032006 1 3731
BBa_K2032006	.	engineered_region	1	2654	.	+	0	ID=BBa_K2032001;Name=BBa_K2032001
BBa_K2032006	.	ribosome_entry_site	2006	2018	.	+	0	ID=annotation2515712;Name=RBS-3\Weak;Parent=BBa_K2032001
BBa_K2032006	.	CDS	1359	1997	.	+	0	ID=annotation2515710;Name=hps;Parent=BBa_K2032001
BBa_K2032006	.	CDS	172	1331	.	+	0	ID=annotation2515705;Name=MDH2;Parent=BBa_K2032001
BBa_K2032006	.	ribosome_entry_site	2012	2015	.	+	0	ID=annotation2515714;Name=RBS;Parent=BBa_K2032001
BBa_K2032006	.	operator	26	42	.	+	0	ID=annotation2515698;Name=lac O1;Parent=BBa_K2032001
BBa_K2032006	.	engineered_region	2006	2018	.	+	0	ID=annotation2515713;Name=BBa_B0032;Parent=BBa_K2032001
BBa_K2032006	.	promoter	43	48	.	+	0	ID=annotation2515699;Name=-10;Parent=BBa_K2032001
BBa_K2032006	.	polyA_site	2706	2719	.	+	0	ID=annotation2515721;Name=polya;Parent=BBa_K2032001
BBa_K2032006	.	ribosome_entry_site	1346	1349	.	+	0	ID=annotation2515709;Name=RBS;Parent=BBa_K2032001
BBa_K2032006	.	CDS	2025	2582	.	+	0	ID=annotation2515715;Name=phi;Parent=BBa_K2032001
BBa_K2032006	.	ribosome_entry_site	70	73	.	+	0	ID=annotation2515702;Name=RBS;Parent=BBa_K2032001
BBa_K2032006	.	ribosome_entry_site	64	76	.	+	0	ID=annotation2515700;Name=RBS-3\Weak;Parent=BBa_K2032001
BBa_K2032006	.	promoter	20	25	.	+	0	ID=annotation2515697;Name=-35;Parent=BBa_K2032001
BBa_K2032006	.	engineered_region	2679	2719	.	+	0	ID=annotation2515719;Name=BBa_B0012;Parent=BBa_K2032001
BBa_K2032006	.	engineered_region	1	54	.	+	0	ID=annotation2515695;Name=BBa_R0011;Parent=BBa_K2032001
BBa_K2032006	.	engineered_region	85	1331	.	+	0	ID=annotation2515706;Name=BBa_K2032003;Parent=BBa_K2032001
BBa_K2032006	.	engineered_region	64	76	.	+	0	ID=annotation2515701;Name=BBa_B0032;Parent=BBa_K2032001
BBa_K2032006	.	engineered_region	2025	2582	.	+	0	ID=annotation2515716;Name=BBa_K1585202;Parent=BBa_K2032001
BBa_K2032006	.	ribosome_entry_site	1340	1352	.	+	0	ID=annotation2515707;Name=RBS-3\Weak;Parent=BBa_K2032001
BBa_K2032006	.	stem_loop	2602	2645	.	+	0	ID=annotation2515718;Name=stem_loop;Parent=BBa_K2032001
BBa_K2032006	.	promoter	91	146	.	+	0	ID=annotation2515703;Name=BBa_R0011;Parent=BBa_K2032001
BBa_K2032006	.	engineered_region	1359	1997	.	+	0	ID=annotation2515711;Name=BBa_K1585201;Parent=BBa_K2032001
BBa_K2032006	.	stem_loop	2686	2705	.	+	0	ID=annotation2515720;Name=T7 TE;Parent=BBa_K2032001
BBa_K2032006	.	engineered_region	1340	1352	.	+	0	ID=annotation2515708;Name=BBa_B0032;Parent=BBa_K2032001
BBa_K2032006	.	operator	3	19	.	+	0	ID=annotation2515696;Name=lac O1;Parent=BBa_K2032001
BBa_K2032006	.	engineered_region	2591	2719	.	+	0	ID=annotation2515723;Name=BBa_B0015;Parent=BBa_K2032001
BBa_K2032006	.	stop_codon	2712	2712	.	+	0	ID=annotation2515722;Name=stop;Parent=BBa_K2032001
BBa_K2032006	.	ribosome_entry_site	153	166	.	+	0	ID=annotation2515704;Name=BBa_B0032;Parent=BBa_K2032001
BBa_K2032006	.	engineered_region	2591	2670	.	+	0	ID=annotation2515717;Name=BBa_B0010;Parent=BBa_K2032001
BBa_K2032006	.	engineered_region	2663	3731	.	+	0	ID=BBa_K801100;Name=BBa_K801100
BBa_K2032006	.	sequence_feature	3603	3731	.	+	0	ID=annotation2213054;Name=Double Terminator BBa_B0015;Parent=BBa_K801100
BBa_K2032006	.	engineered_tag	3570	3594	.	+	0	ID=annotation2213053;Name=Barcode;Parent=BBa_K801100
BBa_K2032006	.	ribosome_entry_site	2871	2882	.	+	0	ID=annotation2213051;Name=RBS (Elowitz 1999) BBa_B0034;Parent=BBa_K801100
BBa_K2032006	.	CDS	2889	3564	.	+	0	ID=annotation2213052;Name=Red Fluorescent Protein from Discosoma striata BBa_E1010;Parent=BBa_K801100
BBa_K2032006	.	promoter	2663	2862	.	+	0	ID=annotation2213050;Name=Promoter (lacI regulated) BBa_R0010;Parent=BBa_K801100
>BBa_K2032006
aagcttaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatacgtatcacacaggaaa
gaggcctatgacaaacacacaatctgcttttttcatgccatcggtgaatctgtttggggctggaagcgtcaatgaggta
ggcacgcgtctggcagacctgggtgtcaaaaaggcattattagttactgatgcaggtttacacggattgggtctttccg
aaaagatttcaagcattattcgcgcagcgggcgtagaagtgtcgattttccccaaagcagagcctaatcccacagataa
aaatgttgcagagggcttagaggcgtataacgcggaaaactgtgactccattgtcacgctgggtggcggttcctcccat
gatgcggggaaagcgattgctcttgttgcggctaacgggggtaagattcatgactacgaaggggtagacgtttcgaaag
agccgatggttcccttgatcgcgatcaataccactgccggaacgggtagcgagctgacaaagttcacgattattactga
tacggaacgcaaagtcaaaatggcaatcgtagataaacatgttactccaaccctttcgatcaatgatccagagcttatg
gttgggatgccaccctcgctgacggccgcgactggcttggacgcacttactcatgcgatcgaggcatacgtttcaaccg
gggcgactcctatcactgacgcgttggcaatccaggcgatcaaaatcatcagtaagtacttaccacgtgctgttgcgaa
tggtaaagacattgaggcccgtgaacaaatggcgttcgctcaatcacttgctggcatggcctttaacaacgcaggttta
ggatacgtgcatgcaatcgcccatcagttggggggattttacaattttccgcatggagtgtgcaacgctgtacttcttc
cctacgtttgtcgctttaatttaatctctaaagtagaacgctatgctgaaatcgcggcgttcttgggagaaaacgtgga
cggcctgagtacatatgatgctgctgagaaggccattaaggctatcgaacgtatggccaaggatcttaacatcccgaag
gggttcaaagaactgggagccaaagaggaagatatcgagactttagccaagaacgcgatgaaggatgcttgtgctctga
caaaccctcgcaagcccaagttggaggaggtcattcagatcatcaaaaacgctatgtgataagtggagatacatacggg
gatcctgatctgtcacacaggaaagaccggtatggagttacaattagctttagatttggttaacatcgaagaggctaaa
caagtggtggctgaagtccaggagtacgtcgatattgttgaaatcgggacccccgtcatcaagatctggggcttacagg
ccgttaaagccgtgaaggatgccttcccacatctgcaagtcttagccgacatgaagaccatggatgctgccgcatacga
ggtggcgaaggccgcggaacatggcgcagatattgttacgatccttgcagcggcagaggatgtatcgattaagggcgcc
gtggaagaagcaaaaaagttagggaaaaaaatcctggtggacatgatcgctgtaaaaaacttagaagaacgtgctaagc
aagttgatgagatgggcgttgactatatctgcgtgcatgcagggtatgacttgcaggctgttggcaagaaccctttaga
tgacctgaagcgcattaaggcggtggtgaagaacgccaagacagccatcgccggtggaatcaagttagagactttacca
gaggttatcaaggctgaacctgacttggtcattgtagggggtggcatcgctaatcagaccgacaagaaggcagccgctg
agaagattaacaaactggtcaagcaagggttgtaataaaatatttcacacaggaaaggccggcatgcttaccaccgagt
tcttagccgaaatcgtaaaagagcttaacagctcagttaatcagattgcggatgaggaagcagaggcacttgttaatgg
tattttgcagagcaaaaaagttttcgttgccggggctggacgttctggcttcatggcaaagagttttgctatgcgtatg
atgcatatgggaatcgacgcttatgtagtgggagaaacagttaccccgaattacgaaaaagaagatatccttatcattg
gatcgggaagtggggaaacgaaatcgcttgtaagtatggcgcagaaagctaagagtatcgggggtactattgccgctgt
cacgatcaacccagagtctacgattgggcaattagcggatatcgtcattaaaatgcctgggagccctaaagataaatca
gaagcgcgcgagacgattcaaccaatgggctccttatttgagcagacattattattgttttatgacgccgtgattctgc
gcttcatggaaaagaagggcttagatacgaagaccatgtatggccgccacgcaaaccttgaataataatactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccg
cgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaa
tgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagc
ggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcat
gcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtac
gaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagt
tccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggttt
caaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgag
ttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttggg
aagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacgg
tggtcactacgacgctgaagttaaaaccacctacatggctaaaaagccggttcagctgccgggtgcttacaaaaccgac
atcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactcca
ccggcgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttata