BBa_K2032006 1 BBa_K2032006 Fructose with RFP 2016-10-11T11:00:00Z 2016-10-19T11:38:27Z M. capsulatus and E. coli Fructose construct with RFP reporter false false _2499_ 26329 26329 9 false Ligation false Chun Mun Loke component2521665 1 BBa_K801100 component2521659 1 BBa_K2032001 annotation2521665 1 BBa_K801100 range2521665 1 2663 3731 annotation2521659 1 BBa_K2032001 range2521659 1 1 2654 BBa_K801100 1 BBa_K801100 RFP Coding Device - RFC10 and RFC25 compatible 2012-10-20T11:00:00Z 2016-10-14T06:54:47Z x x false false _1057_ 31248 14732 9 Not in stock false x false TU Munich annotation2213053 1 Barcode range2213053 1 908 932 annotation2213051 1 RBS (Elowitz 1999) BBa_B0034 range2213051 1 209 220 annotation2213052 1 Red Fluorescent Protein from Discosoma striata BBa_E1010 range2213052 1 227 902 annotation2213054 1 Double Terminator BBa_B0015 range2213054 1 941 1069 annotation2213050 1 Promoter (lacI regulated) BBa_R0010 range2213050 1 1 200 BBa_K2032001 1 BBa_K2032001 Fructose Pathway 2016-08-26T11:00:00Z 2016-10-19T11:25:25Z Methanotrophic bacteria Methylococcus capsulatus Contains genes MDH, HPS, and PHI. Methanol Dehydrogenase II converts methanol into formate. HPS and PHI turn it into metabolites that feed into the glycolysis pathway. (add detail) false false _2499_ 26329 26329 9 false Used Gibson Aseembly false Chun Mun Loke annotation2515712 1 RBS-3\Weak range2515712 1 2006 2018 annotation2515696 1 lac O1 range2515696 1 3 19 annotation2515721 1 polya range2515721 1 2706 2719 annotation2515706 1 BBa_K2032003 range2515706 1 85 1331 annotation2515719 1 BBa_B0012 range2515719 1 2679 2719 annotation2515708 1 BBa_B0032 range2515708 1 1340 1352 annotation2515695 1 BBa_R0011 range2515695 1 1 54 annotation2515699 1 -10 range2515699 1 43 48 annotation2515707 1 RBS-3\Weak range2515707 1 1340 1352 annotation2515711 1 BBa_K1585201 range2515711 1 1359 1997 annotation2515718 1 stem_loop range2515718 1 2602 2645 annotation2515710 1 hps range2515710 1 1359 1997 annotation2515714 1 RBS range2515714 1 2012 2015 annotation2515697 1 -35 range2515697 1 20 25 annotation2515709 1 RBS range2515709 1 1346 1349 annotation2515705 1 MDH2 range2515705 1 172 1331 annotation2515701 1 BBa_B0032 range2515701 1 64 76 annotation2515713 1 BBa_B0032 range2515713 1 2006 2018 annotation2515723 1 BBa_B0015 range2515723 1 2591 2719 annotation2515700 1 RBS-3\Weak range2515700 1 64 76 annotation2515720 1 T7 TE range2515720 1 2686 2705 annotation2515722 1 stop range2515722 1 2712 2712 annotation2515702 1 RBS range2515702 1 70 73 annotation2515717 1 BBa_B0010 range2515717 1 2591 2670 annotation2515704 1 BBa_B0032 range2515704 1 153 166 annotation2515698 1 lac O1 range2515698 1 26 42 annotation2515703 1 BBa_R0011 range2515703 1 91 146 annotation2515715 1 phi range2515715 1 2025 2582 annotation2515716 1 BBa_K1585202 range2515716 1 2025 2582 BBa_K801100_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaagccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggcgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2032001_sequence 1 aagcttaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatacgtatcacacaggaaagaggcctatgacaaacacacaatctgcttttttcatgccatcggtgaatctgtttggggctggaagcgtcaatgaggtaggcacgcgtctggcagacctgggtgtcaaaaaggcattattagttactgatgcaggtttacacggattgggtctttccgaaaagatttcaagcattattcgcgcagcgggcgtagaagtgtcgattttccccaaagcagagcctaatcccacagataaaaatgttgcagagggcttagaggcgtataacgcggaaaactgtgactccattgtcacgctgggtggcggttcctcccatgatgcggggaaagcgattgctcttgttgcggctaacgggggtaagattcatgactacgaaggggtagacgtttcgaaagagccgatggttcccttgatcgcgatcaataccactgccggaacgggtagcgagctgacaaagttcacgattattactgatacggaacgcaaagtcaaaatggcaatcgtagataaacatgttactccaaccctttcgatcaatgatccagagcttatggttgggatgccaccctcgctgacggccgcgactggcttggacgcacttactcatgcgatcgaggcatacgtttcaaccggggcgactcctatcactgacgcgttggcaatccaggcgatcaaaatcatcagtaagtacttaccacgtgctgttgcgaatggtaaagacattgaggcccgtgaacaaatggcgttcgctcaatcacttgctggcatggcctttaacaacgcaggtttaggatacgtgcatgcaatcgcccatcagttggggggattttacaattttccgcatggagtgtgcaacgctgtacttcttccctacgtttgtcgctttaatttaatctctaaagtagaacgctatgctgaaatcgcggcgttcttgggagaaaacgtggacggcctgagtacatatgatgctgctgagaaggccattaaggctatcgaacgtatggccaaggatcttaacatcccgaaggggttcaaagaactgggagccaaagaggaagatatcgagactttagccaagaacgcgatgaaggatgcttgtgctctgacaaaccctcgcaagcccaagttggaggaggtcattcagatcatcaaaaacgctatgtgataagtggagatacatacggggatcctgatctgtcacacaggaaagaccggtatggagttacaattagctttagatttggttaacatcgaagaggctaaacaagtggtggctgaagtccaggagtacgtcgatattgttgaaatcgggacccccgtcatcaagatctggggcttacaggccgttaaagccgtgaaggatgccttcccacatctgcaagtcttagccgacatgaagaccatggatgctgccgcatacgaggtggcgaaggccgcggaacatggcgcagatattgttacgatccttgcagcggcagaggatgtatcgattaagggcgccgtggaagaagcaaaaaagttagggaaaaaaatcctggtggacatgatcgctgtaaaaaacttagaagaacgtgctaagcaagttgatgagatgggcgttgactatatctgcgtgcatgcagggtatgacttgcaggctgttggcaagaaccctttagatgacctgaagcgcattaaggcggtggtgaagaacgccaagacagccatcgccggtggaatcaagttagagactttaccagaggttatcaaggctgaacctgacttggtcattgtagggggtggcatcgctaatcagaccgacaagaaggcagccgctgagaagattaacaaactggtcaagcaagggttgtaataaaatatttcacacaggaaaggccggcatgcttaccaccgagttcttagccgaaatcgtaaaagagcttaacagctcagttaatcagattgcggatgaggaagcagaggcacttgttaatggtattttgcagagcaaaaaagttttcgttgccggggctggacgttctggcttcatggcaaagagttttgctatgcgtatgatgcatatgggaatcgacgcttatgtagtgggagaaacagttaccccgaattacgaaaaagaagatatccttatcattggatcgggaagtggggaaacgaaatcgcttgtaagtatggcgcagaaagctaagagtatcgggggtactattgccgctgtcacgatcaacccagagtctacgattgggcaattagcggatatcgtcattaaaatgcctgggagccctaaagataaatcagaagcgcgcgagacgattcaaccaatgggctccttatttgagcagacattattattgttttatgacgccgtgattctgcgcttcatggaaaagaagggcttagatacgaagaccatgtatggccgccacgcaaaccttgaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2032006_sequence 1 aagcttaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatacgtatcacacaggaaagaggcctatgacaaacacacaatctgcttttttcatgccatcggtgaatctgtttggggctggaagcgtcaatgaggtaggcacgcgtctggcagacctgggtgtcaaaaaggcattattagttactgatgcaggtttacacggattgggtctttccgaaaagatttcaagcattattcgcgcagcgggcgtagaagtgtcgattttccccaaagcagagcctaatcccacagataaaaatgttgcagagggcttagaggcgtataacgcggaaaactgtgactccattgtcacgctgggtggcggttcctcccatgatgcggggaaagcgattgctcttgttgcggctaacgggggtaagattcatgactacgaaggggtagacgtttcgaaagagccgatggttcccttgatcgcgatcaataccactgccggaacgggtagcgagctgacaaagttcacgattattactgatacggaacgcaaagtcaaaatggcaatcgtagataaacatgttactccaaccctttcgatcaatgatccagagcttatggttgggatgccaccctcgctgacggccgcgactggcttggacgcacttactcatgcgatcgaggcatacgtttcaaccggggcgactcctatcactgacgcgttggcaatccaggcgatcaaaatcatcagtaagtacttaccacgtgctgttgcgaatggtaaagacattgaggcccgtgaacaaatggcgttcgctcaatcacttgctggcatggcctttaacaacgcaggtttaggatacgtgcatgcaatcgcccatcagttggggggattttacaattttccgcatggagtgtgcaacgctgtacttcttccctacgtttgtcgctttaatttaatctctaaagtagaacgctatgctgaaatcgcggcgttcttgggagaaaacgtggacggcctgagtacatatgatgctgctgagaaggccattaaggctatcgaacgtatggccaaggatcttaacatcccgaaggggttcaaagaactgggagccaaagaggaagatatcgagactttagccaagaacgcgatgaaggatgcttgtgctctgacaaaccctcgcaagcccaagttggaggaggtcattcagatcatcaaaaacgctatgtgataagtggagatacatacggggatcctgatctgtcacacaggaaagaccggtatggagttacaattagctttagatttggttaacatcgaagaggctaaacaagtggtggctgaagtccaggagtacgtcgatattgttgaaatcgggacccccgtcatcaagatctggggcttacaggccgttaaagccgtgaaggatgccttcccacatctgcaagtcttagccgacatgaagaccatggatgctgccgcatacgaggtggcgaaggccgcggaacatggcgcagatattgttacgatccttgcagcggcagaggatgtatcgattaagggcgccgtggaagaagcaaaaaagttagggaaaaaaatcctggtggacatgatcgctgtaaaaaacttagaagaacgtgctaagcaagttgatgagatgggcgttgactatatctgcgtgcatgcagggtatgacttgcaggctgttggcaagaaccctttagatgacctgaagcgcattaaggcggtggtgaagaacgccaagacagccatcgccggtggaatcaagttagagactttaccagaggttatcaaggctgaacctgacttggtcattgtagggggtggcatcgctaatcagaccgacaagaaggcagccgctgagaagattaacaaactggtcaagcaagggttgtaataaaatatttcacacaggaaaggccggcatgcttaccaccgagttcttagccgaaatcgtaaaagagcttaacagctcagttaatcagattgcggatgaggaagcagaggcacttgttaatggtattttgcagagcaaaaaagttttcgttgccggggctggacgttctggcttcatggcaaagagttttgctatgcgtatgatgcatatgggaatcgacgcttatgtagtgggagaaacagttaccccgaattacgaaaaagaagatatccttatcattggatcgggaagtggggaaacgaaatcgcttgtaagtatggcgcagaaagctaagagtatcgggggtactattgccgctgtcacgatcaacccagagtctacgattgggcaattagcggatatcgtcattaaaatgcctgggagccctaaagataaatcagaagcgcgcgagacgattcaaccaatgggctccttatttgagcagacattattattgttttatgacgccgtgattctgcgcttcatggaaaagaagggcttagatacgaagaccatgtatggccgccacgcaaaccttgaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaagccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggcgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z