LOCUS BBa_K2032007 3525 bp DNA linear 25-Nov-2024 DEFINITION Formate with RFP ACCESSION BBa_K2032007 VERSION BBa_K2032007.1 FEATURES Location/Qualifiers stem_loop 2325..2368 /label=stem_loop misc_feature 2314..2393 /label=BBa_B0010 terminator 2314..2393 /label=BBa_B0010 stem_loop 2409..2428 /label=T7 TE misc_feature 2435..2435 /label=stop polyA_site 2429..2442 /label=polya misc_feature 2402..2442 /label=BBa_B0012 terminator 2402..2442 /label=BBa_B0012 misc_feature 1..1247 /label=BBa_K2032003 misc_feature 3513..3592 /label=BBa_B0010 misc_feature 3601..3641 /label=BBa_B0012 RBS 1257..1269 /label=BBa_B0032 RBS 2352..2364 /label=BBa_B0032 misc_feature 2371..3501 /label=BBa_K1537026 misc_feature 1276..2340 /label=BBa_K1537025 misc_feature 1..2448 /label=BBa_K2032002 CDS 2457..2544 /label=end of LacI coding region (inactive) misc_feature 2457..2656 /label=BBa_R0010 misc_feature 2545..2582 /label=CAP binding site promoter 2593..2598 /label=-35 promoter 2617..2622 /label=-10 misc_feature 2622..2656 /label=LacI binding site misc_feature 2629..2629 /label=start promoter 2457..2656 /label=BBa_R0010 misc_feature 2669..2672 /label=conserved RBS 2665..2676 /label=BBa_B0034 CDS 2683..3357 /label=mrfp1 misc_feature 3364..3388 /label=Help:Barcodes CDS 2683..3363 /label=BBa_E1010 stem_loop 3408..3451 /label=stem_loop misc_feature 3397..3476 /label=BBa_B0010 terminator 3397..3476 /label=BBa_B0010 stem_loop 3492..3511 /label=T7 TE misc_feature 3518..3518 /label=stop polyA_site 3512..3525 /label=polya misc_feature 3485..3525 /label=BBa_B0012 terminator 3485..3525 /label=BBa_B0012 misc_feature 2457..3525 /label=BBa_J04450 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagtcacaca ggaaagtact agatgtcttc aggagatagt aagaaaattg ttggtgtgtt 121 ttataaggct aacgagtacg caactaaaaa tccaaacttc cttggatgtg ttgagaatgc 181 tcttggtatt agagattggt tggaatcaca aggacatcag tatatcgtta cagatgataa 241 ggagggtcca gattgcgaac ttgagaaaca tattcctgat ctccacgttt taatctctac 301 accatttcat cctgcttacg tgaccgcaga aaggattaag aaagctaaga acttgaaact 361 tttgctcacc gcaggaatcg gttcagatca cattgatctt caagctgctg ctgctgctgg 421 acttactgtt gctgaagtga ctggtagtaa cgttgtgtct gttgcagaag atgagttgat 481 gagaattttg attcttatga ggaacttcgt tcctggatat aaccaagttg tgaagggaga 541 gtggaatgtg gcaggtatcg cttatagagc atacgatctt gaaggaaaaa ccataggtac 601 tgttggagct ggtagaattg gaaagttact tttgcagagg ttgaaaccat ttggttgtaa 661 tctcttatac catgataggc tccaaatggc tcctgaatta gagaaggaaa caggtgcaaa 721 attcgttgag gatcttaatg agatgttgcc aaagtgcgat gttatagtga ttaacatgcc 781 tctcacagag aaaaccagag gaatgttcaa taaggaactc ataggaaaat taaagaaagg 841 tgttttgatc gtgaataacg ctagaggagc tattatggaa aggcaggctg ttgtggatgc 901 agttgaatca ggacacatcg gaggttatag tggagatgtt tgggaccctc aaccagctcc 961 taaggatcat ccatggagat acatgcctaa ccaggctatg actccacaca catctggaac 1021 tacaatcgat gctcaactca gatatgcagc tggaactaag gatatgttag agaggtactt 1081 caaaggtgaa gattttccaa ccgagaatta catagtgaag gatggtgaac ttgctccaca 1141 atacaggtac taatagagtc acacaggaaa gtactagatg ggagcagtga cttaccaagg 1201 aaaaaattct atcgctgtta agaaagtgga tgcaccatca attcaggata gagaggatgt 1261 tattatcagg atcacttcta cagctatatg tggatctgat cttcatcttt atcagggtaa 1321 cttccctttg ccaataggat acgttattgg tcacgaacct atgggaattg ttgaagaagt 1381 gggtccagat gttacagctg tgaagaaagg agatagagtt gtgatccctt ttaccgttgc 1441 atgtggtaaa tgccaatatt gtgatcatca tcttgaatca cagtgcgata atagtaaccc 1501 acattatgat agtggaggtt tattcggata ctctgagaag tatggtaatt accctggagg 1561 tcaagctgaa tatctcaggg ttccatttgg aaactacaca cctttcaaaa ttccagatga 1621 ttgcgaattg gaggatgaac agcttttgtt tctctctgat gtgttaccta ccgcttactg 1681 gtctgttgag catgcaggag tgaagaaagg agatacagtt atcgtgctcg gatgtggtcc 1741 agttggactt atggctcaac agttcgcatg gcaaaagggt gctgaaagag ttattgcagt 1801 ggattatatc gattacagac ttaggcacgc taaaaggatg aatggtgttg aggtgtttga 1861 tttcactgaa gatcctgata tgggtgagac acttaaagaa ttgaccaaag gaggtgcaga 1921 tgttgtgatt gattgtgttg gtatggatgg aaagaagtct cctttggaga agatcgaaca 1981 aaaacttaaa cttcaaggag gtactatagg tccaatccaa atagctacaa aggcagttag 2041 aaaaagggga accgttcaga tgactggtgt gtatggaggt ctttacaata tgtttcctct 2101 tggagctttc tttgcaagaa acgttacact taaaatggga caagctccag caaggggtta 2161 tatgtcaaag ttgtaccaga aagtgaccag tggagagatt gatcctagag ctataattac 2221 tcacaaactc ccattagatg aagctgcaca cgcataccat atcttcaacg ataaacagga 2281 tgattgcata aaagtgattc tcaagcctta atactagagc caggcatcaa ataaaacgaa 2341 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2401 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagcaat 2461 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 2521 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 2581 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 2641 ataacaattt cacacatact agagaaagag gagaaatact agatggcttc ctccgaagac 2701 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag 2761 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg 2821 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag 2881 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc 2941 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc 3001 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt 3061 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc 3121 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa 3181 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg 3241 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa 3301 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa 3361 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg 3421 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 3481 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //