BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961227 1 start range1961227 1 173 173 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 BBa_K2032002 1 BBa_K2032002 Formate Pathway 2016-08-26T11:00:00Z 2016-10-19T11:26:27Z methylococcus capsulatus Converts methanol into carbon dioxide, generating energy in the form of NADPH in the process. false false _2499_ 26329 26329 9 false Used Gibson to connect intermediates, except terminator. Terminator was added with ligation. false Jacob Premo, Seth Cohen, Chun Mun Loke annotation2515950 1 BBa_B0010 range2515950 1 3513 3592 annotation2515952 1 BBa_B0032 range2515952 1 1257 1269 annotation2515951 1 BBa_B0012 range2515951 1 3601 3641 annotation2515949 1 BBa_K2032003 range2515949 1 1 1247 annotation2515953 1 BBa_B0032 range2515953 1 2352 2364 annotation2515954 1 BBa_K1537026 range2515954 1 2371 3501 annotation2515955 1 BBa_K1537025 range2515955 1 1276 2340 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K2032007 1 BBa_K2032007 Formate with RFP 2016-10-11T11:00:00Z 2016-10-12T12:21:37Z E. coli et al. Formate construct with RFP reporter false false _2499_ 26329 26329 9 false Ligation false Chun Mun Loke component2493339 1 BBa_K2032002 component2493335 1 BBa_B0012 component2493333 1 BBa_B0010 component2493357 1 BBa_J04450 annotation2493357 1 BBa_J04450 range2493357 1 2457 3525 annotation2493335 1 BBa_B0012 range2493335 1 2402 2442 annotation2493339 1 BBa_K2032002 range2493339 1 1 2448 annotation2493333 1 BBa_B0010 range2493333 1 2314 2393 BBa_J04450 1 BBa_J04450 RFP Coding Device 2005-06-08T11:00:00Z 2015-08-31T04:08:14Z Davidson Synth-Aces Released HQ 2013 Contains an IPTG inducible promoter an RBS, RFP, and terminator. false true _16_ 0 328 16 In stock false true Tamar Odle component1509417 1 BBa_B0010 component1509394 1 BBa_R0010 component1509404 1 BBa_B0034 component1509411 1 BBa_E1010 component1509427 1 BBa_B0012 annotation1509404 1 BBa_B0034 range1509404 1 209 220 annotation1509394 1 BBa_R0010 range1509394 1 1 200 annotation1509411 1 BBa_E1010 range1509411 1 227 907 annotation1509427 1 BBa_B0012 range1509427 1 1029 1069 annotation1509417 1 BBa_B0010 range1509417 1 941 1020 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K2032002_sequence 1 cacgtgaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatgcgcatcacacaggaaagaggcctatgacaaacacacaatctgcttttttcatgccatcggtgaatctgtttggggctggaagcgtcaatgaggtaggcacgcgtctggcagacctgggtgtcaaaaaggcattattagttactgatgcaggtttacacggattgggtctttccgaaaagatttcaagcattattcgcgcagcgggcgtagaagtgtcgattttccccaaagcagagcctaatcccacagataaaaatgttgcagagggcttagaggcgtataacgcggaaaactgtgactccattgtcacgctgggtggcggttcctcccatgatgcggggaaagcgattgctcttgttgcggctaacgggggtaagattcatgactacgaaggggtagacgtttcgaaagagccgatggttcccttgatcgcgatcaataccactgccggaacgggtagcgagctgacaaagttcacgattattactgatacggaacgcaaagtcaaaatggcaatcgtagataaacatgttactccaaccctttcgatcaatgatccagagcttatggttgggatgccaccctcgctgacggccgcgactggcttggacgcacttactcatgcgatcgaggcatacgtttcaaccggggcgactcctatcactgacgcgttggcaatccaggcgatcaaaatcatcagtaagtacttaccacgtgctgttgcgaatggtaaagacattgaggcccgtgaacaaatggcgttcgctcaatcacttgctggcatggcctttaacaacgcaggtttaggatacgtgcatgcaatcgcccatcagttggggggattttacaattttccgcatggagtgtgcaacgctgtacttcttccctacgtttgtcgctttaatttaatctctaaagtagaacgctatgctgaaatcgcggcgttcttgggagaaaacgtggacggcctgagtacatatgatgctgctgagaaggccattaaggctatcgaacgtatggccaaggatcttaacatcccgaaggggttcaaagaactgggagccaaagaggaagatatcgagactttagccaagaacgcgatgaaggatgcttgtgctctgacaaaccctcgcaagcccaagttggaggaggtcattcagatcatcaaaaacgctatgtgataatactagagtcacacaggaaagtactagatgggagcagtgacttaccaaggaaaaaattctatcgctgttaagaaagtggatgcaccatcaattcaggatagagaggatgttattatcaggatcacttctacagctatatgtggatctgatcttcatctttatcagggtaacttccctttgccaataggatacgttattggtcacgaacctatgggaattgttgaagaagtgggtccagatgttacagctgtgaagaaaggagatagagttgtgatcccttttaccgttgcatgtggtaaatgccaatattgtgatcatcatcttgaatcacagtgcgataatagtaacccacattatgatagtggaggtttattcggatactctgagaagtatggtaattaccctggaggtcaagctgaatatctcagggttccatttggaaactacacacctttcaaaattccagatgattgcgaattggaggatgaacagcttttgtttctctctgatgtgttacctaccgcttactggtctgttgagcatgcaggagtgaagaaaggagatacagttatcgtgctcggatgtggtccagttggacttatggctcaacagttcgcatggcaaaagggtgctgaaagagttattgcagtggattatatcgattacagacttaggcacgctaaaaggatgaatggtgttgaggtgtttgatttcactgaagatcctgatatgggtgagacacttaaagaattgaccaaaggaggtgcagatgttgtgattgattgtgttggtatggatggaaagaagtctcctttggagaagatcgaacaaaaacttaaacttcaaggaggtactataggtccaatccaaatagctacaaaggcagttagaaaaaggggaaccgttcagatgactggtgtgtatggaggtctttacaatatgtttcctcttggagctttctttgcaagaaacgttacacttaaaatgggacaagctccagcaaggggttatatgtcaaagttgtaccagaaagtgaccagtggagagattgatcctagagctataattactcacaaactcccattagatgaagctgcacacgcataccatatcttcaacgataaacaggatgattgcataaaagtgattctcaagccttaatactagagtcacacaggaaagtactagatgtcttcaggagatagtaagaaaattgttggtgtgttttataaggctaacgagtacgcaactaaaaatccaaacttccttggatgtgttgagaatgctcttggtattagagattggttggaatcacaaggacatcagtatatcgttacagatgataaggagggtccagattgcgaacttgagaaacatattcctgatctccacgttttaatctctacaccatttcatcctgcttacgtgaccgcagaaaggattaagaaagctaagaacttgaaacttttgctcaccgcaggaatcggttcagatcacattgatcttcaagctgctgctgctgctggacttactgttgctgaagtgactggtagtaacgttgtgtctgttgcagaagatgagttgatgagaattttgattcttatgaggaacttcgttcctggatataaccaagttgtgaagggagagtggaatgtggcaggtatcgcttatagagcatacgatcttgaaggaaaaaccataggtactgttggagctggtagaattggaaagttacttttgcagaggttgaaaccatttggttgtaatctcttataccatgataggctccaaatggctcctgaattagagaaggaaacaggtgcaaaattcgttgaggatcttaatgagatgttgccaaagtgcgatgttatagtgattaacatgcctctcacagagaaaaccagaggaatgttcaataaggaactcataggaaaattaaagaaaggtgttttgatcgtgaataacgctagaggagctattatggaaaggcaggctgttgtggatgcagttgaatcaggacacatcggaggttatagtggagatgtttgggaccctcaaccagctcctaaggatcatccatggagatacatgcctaaccaggctatgactccacacacatctggaactacaatcgatgctcaactcagatatgcagctggaactaaggatatgttagagaggtacttcaaaggtgaagattttccaaccgagaattacatagtgaaggatggtgaacttgctccacaatacaggtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_J04450_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K2032007_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtactagatgtcttcaggagatagtaagaaaattgttggtgtgttttataaggctaacgagtacgcaactaaaaatccaaacttccttggatgtgttgagaatgctcttggtattagagattggttggaatcacaaggacatcagtatatcgttacagatgataaggagggtccagattgcgaacttgagaaacatattcctgatctccacgttttaatctctacaccatttcatcctgcttacgtgaccgcagaaaggattaagaaagctaagaacttgaaacttttgctcaccgcaggaatcggttcagatcacattgatcttcaagctgctgctgctgctggacttactgttgctgaagtgactggtagtaacgttgtgtctgttgcagaagatgagttgatgagaattttgattcttatgaggaacttcgttcctggatataaccaagttgtgaagggagagtggaatgtggcaggtatcgcttatagagcatacgatcttgaaggaaaaaccataggtactgttggagctggtagaattggaaagttacttttgcagaggttgaaaccatttggttgtaatctcttataccatgataggctccaaatggctcctgaattagagaaggaaacaggtgcaaaattcgttgaggatcttaatgagatgttgccaaagtgcgatgttatagtgattaacatgcctctcacagagaaaaccagaggaatgttcaataaggaactcataggaaaattaaagaaaggtgttttgatcgtgaataacgctagaggagctattatggaaaggcaggctgttgtggatgcagttgaatcaggacacatcggaggttatagtggagatgtttgggaccctcaaccagctcctaaggatcatccatggagatacatgcctaaccaggctatgactccacacacatctggaactacaatcgatgctcaactcagatatgcagctggaactaaggatatgttagagaggtacttcaaaggtgaagattttccaaccgagaattacatagtgaaggatggtgaacttgctccacaatacaggtactaatagagtcacacaggaaagtactagatgggagcagtgacttaccaaggaaaaaattctatcgctgttaagaaagtggatgcaccatcaattcaggatagagaggatgttattatcaggatcacttctacagctatatgtggatctgatcttcatctttatcagggtaacttccctttgccaataggatacgttattggtcacgaacctatgggaattgttgaagaagtgggtccagatgttacagctgtgaagaaaggagatagagttgtgatcccttttaccgttgcatgtggtaaatgccaatattgtgatcatcatcttgaatcacagtgcgataatagtaacccacattatgatagtggaggtttattcggatactctgagaagtatggtaattaccctggaggtcaagctgaatatctcagggttccatttggaaactacacacctttcaaaattccagatgattgcgaattggaggatgaacagcttttgtttctctctgatgtgttacctaccgcttactggtctgttgagcatgcaggagtgaagaaaggagatacagttatcgtgctcggatgtggtccagttggacttatggctcaacagttcgcatggcaaaagggtgctgaaagagttattgcagtggattatatcgattacagacttaggcacgctaaaaggatgaatggtgttgaggtgtttgatttcactgaagatcctgatatgggtgagacacttaaagaattgaccaaaggaggtgcagatgttgtgattgattgtgttggtatggatggaaagaagtctcctttggagaagatcgaacaaaaacttaaacttcaaggaggtactataggtccaatccaaatagctacaaaggcagttagaaaaaggggaaccgttcagatgactggtgtgtatggaggtctttacaatatgtttcctcttggagctttctttgcaagaaacgttacacttaaaatgggacaagctccagcaaggggttatatgtcaaagttgtaccagaaagtgaccagtggagagattgatcctagagctataattactcacaaactcccattagatgaagctgcacacgcataccatatcttcaacgataaacaggatgattgcataaaagtgattctcaagccttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z