LOCUS BBa_K2032008 6494 bp DNA linear 29-Nov-2024 DEFINITION sMMO and GFP ACCESSION BBa_K2032008 VERSION BBa_K2032008.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..76 /label=RBS-3\Weak RBS 70..73 /label=RBS misc_feature 64..76 /label=BBa_B0032 RBS 64..76 /label=BBa_B0032 misc_feature 83..508 /label=BBa_K1390001 RBS 517..529 /label=RBS-3\Weak RBS 523..526 /label=RBS misc_feature 517..529 /label=BBa_B0032 RBS 517..529 /label=BBa_B0032 misc_feature 536..1582 /label=BBa_K1390002 RBS 1591..1603 /label=RBS-3\Weak RBS 1597..1600 /label=RBS misc_feature 1591..1603 /label=BBa_B0032 RBS 1591..1603 /label=BBa_B0032 misc_feature 1610..1921 /label=BBa_K1390003 RBS 1930..1942 /label=RBS-3\Weak RBS 1936..1939 /label=RBS misc_feature 1930..1942 /label=BBa_B0032 RBS 1930..1942 /label=BBa_B0032 misc_feature 1949..3532 /label=BBa_K1390004 RBS 3541..3553 /label=RBS-3\Weak RBS 3547..3550 /label=RBS misc_feature 3541..3553 /label=BBa_B0032 RBS 3541..3553 /label=BBa_B0032 misc_feature 3560..4729 /label=BBa_K1390005 RBS 4738..4750 /label=RBS-3\Weak RBS 4744..4747 /label=RBS misc_feature 4738..4750 /label=BBa_B0032 RBS 4738..4750 /label=BBa_B0032 misc_feature 4757..5266 /label=BBa_K1390006 stem_loop 5286..5329 /label=stem_loop misc_feature 5275..5354 /label=BBa_B0010 terminator 5275..5354 /label=BBa_B0010 stem_loop 5370..5389 /label=T7 TE misc_feature 5396..5396 /label=stop polyA_site 5390..5403 /label=polya misc_feature 5363..5403 /label=BBa_B0012 terminator 5363..5403 /label=BBa_B0012 terminator 5275..5403 /label=BBa_B0015 misc_feature 1..5403 /label=BBa_K2032000 CDS 5412..5499 /label=end of LacI coding region (inactive) misc_feature 5412..5611 /label=BBa_R0010 misc_feature 5500..5537 /label=CAP binding site promoter 5548..5553 /label=-35 promoter 5572..5577 /label=-10 misc_feature 5577..5611 /label=LacI binding site misc_feature 5584..5584 /label=start promoter 5412..5611 /label=BBa_R0010 misc_feature 5624..5627 /label=conserved RBS 5620..5631 /label=BBa_B0034 misc_feature 5638..5640 /label=Start CDS 5638..6357 /label=GFP Coding Region CDS 5638..6357 /label=BBa_K208000 stem_loop 6377..6420 /label=stem_loop misc_feature 6366..6445 /label=BBa_B0010 terminator 6366..6445 /label=BBa_B0010 stem_loop 6461..6480 /label=T7 TE misc_feature 6487..6487 /label=stop polyA_site 6481..6494 /label=polya misc_feature 6454..6494 /label=BBa_B0012 terminator 6454..6494 /label=BBa_B0012 misc_feature 5412..6494 /label=BBa_K208045 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagtcacaca ggaaagtact agatgagcgt aaacagcaac gcatacgacg ccggcatcat 121 gggcctgaaa ggcaaggact tcgccgatca gttctttgcc gacgaaaacc aagtggtcca 181 tgaaagcgac acggtcgttc tggtcctcaa gaagtcggac gagatcaata cctttatcga 241 ggagatcctt ctgacggact acaagaagaa cgtcaatccg acggtaaacg tggaagaccg 301 cgcgggttac tggtggatca aggccaacgg caagatcgag gtcgattgcg acgagatttc 361 cgagctgttg gggcggcagt tcaacgtcta cgacttcctc gtcgacgttt cctccaccat 421 cggccgggcc tataccctgg gcaacaagtt caccattacc agtgagctga tgggcctgga 481 ccgcaagctc gaagactatc acgcttaata ctagagtcac acaggaaagt actagatgca 541 gcgagttcac actatcacgg cggtgacgga ggatggcgaa tcgctccgct tcgaatgccg 601 ttcggacgag gacgtcatca ccgccgccct gcgccagaac atctttctga tgtcgtcctg 661 ccgggagggc ggctgtgcga cctgcaaggc cttgtgcagc gaaggggact acgacctcaa 721 gggctgtagc gttcaggcgc tgccgccgga agaggaggag gaagggttgg tgttgttgtg 781 ccggacctac ccgaagaccg acctggaaat cgaactgccc tatacccatt gccgcatcag 841 ttttggtgag gtcggcagtt tcgaggcgga ggtcgtcggc ctcaactggg tttcgagcaa 901 caccgtccag tttcttttgc agaagcggcc cgacgagtgc ggcaaccgtg gcgtgaaatt 961 cgaacccggt cagttcatgg acctgaccat ccccggcacc gatgtctccc gctcctactc 1021 gccggcgaac cttcctaatc ccgaaggccg cctggagttc ctgatccgcg tgttaccgga 1081 gggacggttt tcggactacc tgcgcaatga cgcgcgtgtc ggacaggtcc tctcggtcaa 1141 agggccactg ggcgtgttcg gtctcaagga gcggggcatg gcgccgcgct atttcgtggc 1201 cggcggcacc gggttggcgc cggtggtctc gatggtgcgg cagatgcagg agtggaccgc 1261 gccgaacgag acccgcatct atttcggtgt gaacaccgag ccggaattgt tctacatcga 1321 cgagctcaaa tccctggaac gatcgatgcg caatctcacc gtgaaggcct gtgtctggca 1381 cccgagcggg gactgggaag gcgagcaggg ctcgcccatc gatgcgttgc gggaagacct 1441 ggagtcctcc gacgccaacc cggacattta tttgtgcggt ccgccgggca tgatcgatgc 1501 cgcctgcgag ctggtacgca gccgcggtat ccccggcgaa caggtcttct tcgaaaaatt 1561 cctgccgtcc ggggcggcct gatactagag tcacacagga aagtactaga tggtcgaatc 1621 ggcatttcag ccattttcgg gcgacgcaga cgaatggttc gaggaaccac ggccccaggc 1681 cggtttcttc ccttccgcgg actggcatct gctcaaacgg gacgagacct acgcagccta 1741 tgccaaggat ctcgatttca tgtggcggtg ggtcatcgtc cgggaagaaa ggatcgtcca 1801 ggagggttgc tcgatcagcc tggagtcgtc gatccgcgcc gtgacgcacg tactgaatta 1861 ttttggtatg accgaacaac gcgccccggc agaggaccgg accggcggag ttcaacattg 1921 atactagagt cacacaggaa agtactagat ggcacttagc accgcaacca aggccgcgac 1981 ggacgcgctg gctgccaatc gggcacccac cagcgtgaat gcacaggaag tgcaccgttg 2041 gctccagagc ttcaactggg atttcaagaa caaccggacc aagtacgcca ccaagtacaa 2101 gatggcgaac gagaccaagg aacagttcaa gctgatcgcc aaggaatatg cgcgcatgga 2161 ggcagtcaag gacgaaaggc agttcggtag cctgcaagat gcgctgaccc gcctcaacgc 2221 cggtgttcgc gttcatccga agtggaacga gaccatgaaa gtggtttcga acttcctgga 2281 agtgggcgaa tacaacgcca tcgccgctac cgggatgctg tgggattccg cccaggcggc 2341 ggaacagaag aacggctatc tggcccaggt gttggatgaa atccgccaca cccaccagtg 2401 tgcctacgtc aactactact tcgcgaagaa cggccaggac ccggccggtc acaacgatgc 2461 tcgccgcacc cgtaccatcg gtccgctgtg gaagggcatg aagcgcgtgt tttccgacgg 2521 cttcatttcc ggcgacgccg tggaatgctc cctcaacctg caactggtgg gtgaggcctg 2581 cttcaccaat ccgctgatcg tcgcagtgac cgaatgggct gccgccaacg gcgatgaaat 2641 caccccgacg gtgttcctgt cgatcgagac cgacgaactg cgccacatgg ccaacggtta 2701 ccagaccgtc gtttccatcg ccaacgatcc ggcttccgcc aagtatctca acacggacct 2761 gaacaacgcc ttctggaccc agcagaagta cttcacgccg gtgttgggca tgctgttcga 2821 gtatggctcc aagttcaagg tcgagccgtg ggtcaagacg tggaaccgct gggtgtacga 2881 ggactggggc ggcatctgga tcggccgtct gggcaagtac ggggtggagt cgccgcgcag 2941 cctcaaggac gccaagcagg acgcttactg ggctcaccac gacctgtatc tgctggctta 3001 tgcgctgtgg ccgaccggct tcttccgtct ggcgctgccg gatcaggaag aaatggagtg 3061 gttcgaggcc aactaccccg gctggtacga ccactacggc aagatctacg aggaatggcg 3121 cgcccgcggt tgcgaggatc cgtcctcggg cttcatcccg ctgatgtggt tcatcgaaaa 3181 caaccatccc atctacatcg atcgcgtgtc gcaagtgccg ttctgcccga gcttggccaa 3241 gggcgccagc accctgcgcg tgcacgagta caacggccag atgcacacct tcagcgacca 3301 gtggggcgag cgcatgtggc tggccgagcc ggagcgctac gagtgccaga acatcttcga 3361 acagtacgaa ggacgcgaac tgtcggaagt gatcgccgaa ctgcacgggc tgcgcagtga 3421 tggcaagacc ctgatcgccc agccgcatgt ccgtggcgac aagctgtgga cgttggacga 3481 tatcaaacgc ctgaactgcg tcttcaagaa cccggtgaag gcattcaatt gatactagag 3541 tcacacagga aagtactaga tgagcatgtt aggagaaaga cgccgcggtc tgaccgatcc 3601 ggaaatggcg gccgtcattt tgaaggcgct tcctgaagct ccgctggacg gcaacaacaa 3661 gatgggttat ttcgtcaccc cccgctggaa acgcttgacg gaatatgaag ccctgaccgt 3721 ttatgcgcag cccaacgccg actggatcgc cggcggcctg gactggggcg actggaccca 3781 gaaattccac ggcggccgcc cttcctgggg caacgagacc acggagctgc gcaccgtcga 3841 ctggttcaag caccgtgacc cgctccgccg ttggcatgcg ccgtacgtca aggacaaggc 3901 cgaggaatgg cgctacaccg accgcttcct gcaaggttac tccgccgacg gtcagatccg 3961 ggcgatgaac ccgacctggc gggacgagtt catcaaccgg tattggggcg ccttcctgtt 4021 caacgaatac ggattgttca acgctcattc gcagggcgcc cgggaggcgc tgtcggacgt 4081 aacccgcgtc agcctggctt tctggggctt cgacaagatc gacatcgccc agatgatcca 4141 actcgaacgg ggtttcctcg ccaagatcgt acccggtttc gacgagtcca cagcggtgcc 4201 gaaggccgaa tggacgaacg gggaggtcta caagagcgcc cgtctggccg tggaagggct 4261 gtggcaggag gtgttcgact ggaacgagag cgctttctcg gtgcacgccg tctatgacgc 4321 gctgttcggt cagttcgtcc gccgcgagtt ctttcagcgg ctggctcccc gcttcggcga 4381 caatctgacg ccattcttca tcaaccaggc ccagacatac ttccagatcg ccaagcaggg 4441 cgtacaggat ctgtattaca actgtctggg tgacgatccg gagttcagcg attacaaccg 4501 taccgtgatg cgcaactgga ccggcaagtg gctggagccc acgatcgccg ctctgcgcga 4561 cttcatgggg ctgtttgcga agctgccggc gggcaccact gacaaggaag aaatcaccgc 4621 gtccctgtac cgggtggtcg acgactggat cgaggactac gccagcagga tcgacttcaa 4681 ggcggaccgc gatcagatcg ttaaagcggt tctggcagga ttgaaatgat actagagtca 4741 cacaggaaag tactagatgg cgaaactggg tatacacagc aacgacaccc gcgacgcctg 4801 ggtgaacaag atcgcgcagc tcaacaccct ggaaaaagcg gccgagatgc tgaagcagtt 4861 ccggatggac cacaccacgc cgttccgcaa cagctacgaa ctggacaacg actacctctg 4921 gatcgaggcc aagctcgaag agaaggtcgc cgtcctcaag gcacgcgcct tcaacgaggt 4981 ggacttccgt cataagaccg ctttcggcga ggatgccaag tccgttctgg acggcaccgt 5041 cgcgaagatg aacgcggcca aggacaagtg ggaggcggag aagatccata tcggtttccg 5101 ccaggcctac aagccgccga tcatgccggt gaactatttc ctggacggcg agcgtcagtt 5161 ggggacccgg ctgatggaac tgcgcaacct caactactac gacacgccgc tggaagaact 5221 gcgcaaacag cgcggtgtgc gggtggtgca tctgcaatcg ccgtgatact agagccaggc 5281 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 5341 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 5401 atatactaga gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 5461 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 5521 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 5581 tggaattgtg agcggataac aatttcacac atactagaga aagaggagaa atactagatg 5641 gctagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt 5701 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc tacatacgga 5761 aagcttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt 5821 gtcactactt tctcttatgg tgttcaatgc ttttcccgtt atccggatca tatgaaacgg 5881 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc 5941 aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt 6001 aatcgtatcg agttaaaagg tattgatttt aaagaagatg gaaacattct cggacacaaa 6061 ctcgagtaca actataactc acacaatgta tacatcacgg cagacaaaca aaagaatgga 6121 atcaaagcta acttcaaaat tcgccacaac attgaagatg gatccgttca actagcagac 6181 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac 6241 ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt 6301 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaataatac 6361 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 6421 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 6481 tttctgcgtt tata //