##gff-version 3
##sequence-region BBa_K2032008 1 6494
BBa_K2032008	.	engineered_region	5412	6494	.	+	0	ID=BBa_K208045;Name=BBa_K208045
BBa_K2032008	.	promoter	5412	5611	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K208045
BBa_K2032008	.	CDS	5412	5499	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K2032008	.	promoter	5572	5577	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K2032008	.	non_covalent_binding_site	5577	5611	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K2032008	.	engineered_region	5412	5611	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K2032008	.	promoter	5548	5553	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K2032008	.	non_covalent_binding_site	5500	5537	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K2032008	.	start_codon	5584	5584	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K2032008	.	terminator	6454	6494	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K208045
BBa_K2032008	.	stem_loop	6461	6480	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2032008	.	polyA_site	6481	6494	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2032008	.	stop_codon	6487	6487	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2032008	.	engineered_region	6454	6494	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2032008	.	terminator	6366	6445	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K208045
BBa_K2032008	.	stem_loop	6377	6420	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2032008	.	engineered_region	6366	6445	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2032008	.	CDS	5638	6357	.	+	0	ID=BBa_K208000;Name=BBa_K208000;Parent=BBa_K208045
BBa_K2032008	.	start_codon	5638	5640	.	+	0	ID=annotation2062216;Name=Start;Parent=BBa_K208000
BBa_K2032008	.	CDS	5638	6357	.	+	0	ID=annotation2062217;Name=GFP Coding Region;Parent=BBa_K208000
BBa_K2032008	.	ribosome_entry_site	5620	5631	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K208045
BBa_K2032008	.	conserved_region	5624	5627	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2032008	.	engineered_region	1	5403	.	+	0	ID=BBa_K2032000;Name=BBa_K2032000
BBa_K2032008	.	ribosome_entry_site	4738	4750	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K2032000
BBa_K2032008	.	engineered_region	4738	4750	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	4744	4747	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	4738	4750	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	3541	3553	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K2032000
BBa_K2032008	.	engineered_region	3541	3553	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	3547	3550	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	3541	3553	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	517	529	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K2032000
BBa_K2032008	.	engineered_region	517	529	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	523	526	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	517	529	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K2032008	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K2032000
BBa_K2032008	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K2032008	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K2032008	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K2032008	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K2032008	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K2032008	.	polypeptide_domain	4757	5266	.	+	0	ID=BBa_K1390006;Name=BBa_K1390006;Parent=BBa_K2032000
BBa_K2032008	.	terminator	5275	5403	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K2032000
BBa_K2032008	.	terminator	5363	5403	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2032008	.	stem_loop	5370	5389	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2032008	.	polyA_site	5390	5403	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2032008	.	stop_codon	5396	5396	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2032008	.	engineered_region	5363	5403	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2032008	.	terminator	5275	5354	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2032008	.	stem_loop	5286	5329	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2032008	.	engineered_region	5275	5354	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2032008	.	polypeptide_domain	1949	3532	.	+	0	ID=BBa_K1390004;Name=BBa_K1390004;Parent=BBa_K2032000
BBa_K2032008	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K2032000
BBa_K2032008	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K2032008	.	polypeptide_domain	83	508	.	+	0	ID=BBa_K1390001;Name=BBa_K1390001;Parent=BBa_K2032000
BBa_K2032008	.	polypeptide_domain	1610	1921	.	+	0	ID=BBa_K1390003;Name=BBa_K1390003;Parent=BBa_K2032000
BBa_K2032008	.	polypeptide_domain	3560	4729	.	+	0	ID=BBa_K1390005;Name=BBa_K1390005;Parent=BBa_K2032000
BBa_K2032008	.	ribosome_entry_site	1930	1942	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K2032000
BBa_K2032008	.	engineered_region	1930	1942	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	1936	1939	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	1930	1942	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K2032008	.	polypeptide_domain	536	1582	.	+	0	ID=BBa_K1390002;Name=BBa_K1390002;Parent=BBa_K2032000
BBa_K2032008	.	ribosome_entry_site	1591	1603	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K2032000
BBa_K2032008	.	engineered_region	1591	1603	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	1597	1600	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K2032008	.	ribosome_entry_site	1591	1603	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
>BBa_K2032008
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtac
tagatgagcgtaaacagcaacgcatacgacgccggcatcatgggcctgaaaggcaaggacttcgccgatcagttctttg
ccgacgaaaaccaagtggtccatgaaagcgacacggtcgttctggtcctcaagaagtcggacgagatcaatacctttat
cgaggagatccttctgacggactacaagaagaacgtcaatccgacggtaaacgtggaagaccgcgcgggttactggtgg
atcaaggccaacggcaagatcgaggtcgattgcgacgagatttccgagctgttggggcggcagttcaacgtctacgact
tcctcgtcgacgtttcctccaccatcggccgggcctataccctgggcaacaagttcaccattaccagtgagctgatggg
cctggaccgcaagctcgaagactatcacgcttaatactagagtcacacaggaaagtactagatgcagcgagttcacact
atcacggcggtgacggaggatggcgaatcgctccgcttcgaatgccgttcggacgaggacgtcatcaccgccgccctgc
gccagaacatctttctgatgtcgtcctgccgggagggcggctgtgcgacctgcaaggccttgtgcagcgaaggggacta
cgacctcaagggctgtagcgttcaggcgctgccgccggaagaggaggaggaagggttggtgttgttgtgccggacctac
ccgaagaccgacctggaaatcgaactgccctatacccattgccgcatcagttttggtgaggtcggcagtttcgaggcgg
aggtcgtcggcctcaactgggtttcgagcaacaccgtccagtttcttttgcagaagcggcccgacgagtgcggcaaccg
tggcgtgaaattcgaacccggtcagttcatggacctgaccatccccggcaccgatgtctcccgctcctactcgccggcg
aaccttcctaatcccgaaggccgcctggagttcctgatccgcgtgttaccggagggacggttttcggactacctgcgca
atgacgcgcgtgtcggacaggtcctctcggtcaaagggccactgggcgtgttcggtctcaaggagcggggcatggcgcc
gcgctatttcgtggccggcggcaccgggttggcgccggtggtctcgatggtgcggcagatgcaggagtggaccgcgccg
aacgagacccgcatctatttcggtgtgaacaccgagccggaattgttctacatcgacgagctcaaatccctggaacgat
cgatgcgcaatctcaccgtgaaggcctgtgtctggcacccgagcggggactgggaaggcgagcagggctcgcccatcga
tgcgttgcgggaagacctggagtcctccgacgccaacccggacatttatttgtgcggtccgccgggcatgatcgatgcc
gcctgcgagctggtacgcagccgcggtatccccggcgaacaggtcttcttcgaaaaattcctgccgtccggggcggcct
gatactagagtcacacaggaaagtactagatggtcgaatcggcatttcagccattttcgggcgacgcagacgaatggtt
cgaggaaccacggccccaggccggtttcttcccttccgcggactggcatctgctcaaacgggacgagacctacgcagcc
tatgccaaggatctcgatttcatgtggcggtgggtcatcgtccgggaagaaaggatcgtccaggagggttgctcgatca
gcctggagtcgtcgatccgcgccgtgacgcacgtactgaattattttggtatgaccgaacaacgcgccccggcagagga
ccggaccggcggagttcaacattgatactagagtcacacaggaaagtactagatggcacttagcaccgcaaccaaggcc
gcgacggacgcgctggctgccaatcgggcacccaccagcgtgaatgcacaggaagtgcaccgttggctccagagcttca
actgggatttcaagaacaaccggaccaagtacgccaccaagtacaagatggcgaacgagaccaaggaacagttcaagct
gatcgccaaggaatatgcgcgcatggaggcagtcaaggacgaaaggcagttcggtagcctgcaagatgcgctgacccgc
ctcaacgccggtgttcgcgttcatccgaagtggaacgagaccatgaaagtggtttcgaacttcctggaagtgggcgaat
acaacgccatcgccgctaccgggatgctgtgggattccgcccaggcggcggaacagaagaacggctatctggcccaggt
gttggatgaaatccgccacacccaccagtgtgcctacgtcaactactacttcgcgaagaacggccaggacccggccggt
cacaacgatgctcgccgcacccgtaccatcggtccgctgtggaagggcatgaagcgcgtgttttccgacggcttcattt
ccggcgacgccgtggaatgctccctcaacctgcaactggtgggtgaggcctgcttcaccaatccgctgatcgtcgcagt
gaccgaatgggctgccgccaacggcgatgaaatcaccccgacggtgttcctgtcgatcgagaccgacgaactgcgccac
atggccaacggttaccagaccgtcgtttccatcgccaacgatccggcttccgccaagtatctcaacacggacctgaaca
acgccttctggacccagcagaagtacttcacgccggtgttgggcatgctgttcgagtatggctccaagttcaaggtcga
gccgtgggtcaagacgtggaaccgctgggtgtacgaggactggggcggcatctggatcggccgtctgggcaagtacggg
gtggagtcgccgcgcagcctcaaggacgccaagcaggacgcttactgggctcaccacgacctgtatctgctggcttatg
cgctgtggccgaccggcttcttccgtctggcgctgccggatcaggaagaaatggagtggttcgaggccaactaccccgg
ctggtacgaccactacggcaagatctacgaggaatggcgcgcccgcggttgcgaggatccgtcctcgggcttcatcccg
ctgatgtggttcatcgaaaacaaccatcccatctacatcgatcgcgtgtcgcaagtgccgttctgcccgagcttggcca
agggcgccagcaccctgcgcgtgcacgagtacaacggccagatgcacaccttcagcgaccagtggggcgagcgcatgtg
gctggccgagccggagcgctacgagtgccagaacatcttcgaacagtacgaaggacgcgaactgtcggaagtgatcgcc
gaactgcacgggctgcgcagtgatggcaagaccctgatcgcccagccgcatgtccgtggcgacaagctgtggacgttgg
acgatatcaaacgcctgaactgcgtcttcaagaacccggtgaaggcattcaattgatactagagtcacacaggaaagta
ctagatgagcatgttaggagaaagacgccgcggtctgaccgatccggaaatggcggccgtcattttgaaggcgcttcct
gaagctccgctggacggcaacaacaagatgggttatttcgtcaccccccgctggaaacgcttgacggaatatgaagccc
tgaccgtttatgcgcagcccaacgccgactggatcgccggcggcctggactggggcgactggacccagaaattccacgg
cggccgcccttcctggggcaacgagaccacggagctgcgcaccgtcgactggttcaagcaccgtgacccgctccgccgt
tggcatgcgccgtacgtcaaggacaaggccgaggaatggcgctacaccgaccgcttcctgcaaggttactccgccgacg
gtcagatccgggcgatgaacccgacctggcgggacgagttcatcaaccggtattggggcgccttcctgttcaacgaata
cggattgttcaacgctcattcgcagggcgcccgggaggcgctgtcggacgtaacccgcgtcagcctggctttctggggc
ttcgacaagatcgacatcgcccagatgatccaactcgaacggggtttcctcgccaagatcgtacccggtttcgacgagt
ccacagcggtgccgaaggccgaatggacgaacggggaggtctacaagagcgcccgtctggccgtggaagggctgtggca
ggaggtgttcgactggaacgagagcgctttctcggtgcacgccgtctatgacgcgctgttcggtcagttcgtccgccgc
gagttctttcagcggctggctccccgcttcggcgacaatctgacgccattcttcatcaaccaggcccagacatacttcc
agatcgccaagcagggcgtacaggatctgtattacaactgtctgggtgacgatccggagttcagcgattacaaccgtac
cgtgatgcgcaactggaccggcaagtggctggagcccacgatcgccgctctgcgcgacttcatggggctgtttgcgaag
ctgccggcgggcaccactgacaaggaagaaatcaccgcgtccctgtaccgggtggtcgacgactggatcgaggactacg
ccagcaggatcgacttcaaggcggaccgcgatcagatcgttaaagcggttctggcaggattgaaatgatactagagtca
cacaggaaagtactagatggcgaaactgggtatacacagcaacgacacccgcgacgcctgggtgaacaagatcgcgcag
ctcaacaccctggaaaaagcggccgagatgctgaagcagttccggatggaccacaccacgccgttccgcaacagctacg
aactggacaacgactacctctggatcgaggccaagctcgaagagaaggtcgccgtcctcaaggcacgcgccttcaacga
ggtggacttccgtcataagaccgctttcggcgaggatgccaagtccgttctggacggcaccgtcgcgaagatgaacgcg
gccaaggacaagtgggaggcggagaagatccatatcggtttccgccaggcctacaagccgccgatcatgccggtgaact
atttcctggacggcgagcgtcagttggggacccggctgatggaactgcgcaacctcaactactacgacacgccgctgga
agaactgcgcaaacagcgcggtgtgcgggtggtgcatctgcaatcgccgtgatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattca
ttaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcact
cattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcaca
catactagagaaagaggagaaatactagatggctagcaaaggagaagaacttttcactggagttgtcccaattcttgtt
gaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagctta
cccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttca
atgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaa
cgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatc
gtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcaca
caatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatgga
tccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacc
tgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgc
tgggattacacatggcatggatgagctctacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata