LOCUS       BBa_K2033011             960 bp    DNA     linear       28-Sep-2024
DEFINITION  N-Acyl Homoserine Lactone (AHL) Modular Sender Vector
ACCESSION   BBa_K2033011
VERSION     BBa_K2033011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    87..90
                     /label=conserved
     RBS             83..94
                     /label=BBa_B0034
     CDS             101..808
                     /label=mCherry
     misc_feature    452..452
                     /label=C->T (removing PstI site)
     misc_feature    809..817
                     /label=LVA
     misc_feature    101..823
                     /label=BBa_J06505
     stem_loop       843..886
                     /label=stem_loop
     misc_feature    832..911
                     /label=BBa_B0010
     terminator      832..911
                     /label=BBa_B0010
     stem_loop       927..946
                     /label=T7 TE
     misc_feature    953..953
                     /label=stop
     polyA_site      947..960
                     /label=polya
     misc_feature    920..960
                     /label=BBa_B0012
     terminator      920..960
                     /label=BBa_B0012
     terminator      832..960
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag agaaagagga gaaatactag atggtgagca agggcgagga
      121 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
      181 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      241 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
      301 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
      361 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      421 cggcgtggtg accgtgaccc aggactcctc cttgcaggac ggcgagttca tctacaaggt
      481 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
      541 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
      601 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
      661 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
      721 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
      781 caccggcggc atggacgagc tgtacaagtt agtagcttaa taatactaga gccaggcatc
      841 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      901 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//