##gff-version 3
##sequence-region BBa_K2033011 1 960
BBa_K2033011	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2033011	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2033011	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2033011	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2033011	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2033011	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2033011	.	terminator	832	960	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2033011	.	terminator	920	960	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2033011	.	engineered_region	920	960	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2033011	.	stop_codon	953	953	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2033011	.	polyA_site	947	960	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2033011	.	stem_loop	927	946	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2033011	.	terminator	832	911	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2033011	.	engineered_region	832	911	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2033011	.	stem_loop	843	886	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2033011	.	engineered_region	101	823	.	+	0	ID=BBa_J06505;Name=BBa_J06505
BBa_K2033011	.	tag	809	817	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_K2033011	.	CDS	101	808	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_K2033011	.	sequence_alteration	452	452	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_K2033011	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2033011	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2033011	.	ribosome_entry_site	83	94	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2033011	.	conserved_region	87	90	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K2033011
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttca
aggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcac
ccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtac
ggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtggg
agcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatcta
caaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcc
tccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccact
acgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagtt
ggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggc
atggacgagctgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttata