BBa_K2036010 1 BBa_K2036010 Cro-TT-pRM-RBS-GFP-LVAssrAtag 2016-09-18T11:00:00Z 2016-09-24T05:04:23Z GFP-LVA tag and TT are all standards parts from Registry. And Cro pRM are from bacteriaphage lambda. Cro-TT-pRM-RBS-GFP-LVAtag, it is a negtive control of GFP expression. When inserted into a expression plasmid behind an inducible promoter,it will form a NO gate: GFP will not be produced when there is an induction. false false _2503_ 28503 25901 9 false One should make sure there is an RBS when constructing the expression plasmid else the repression effect will not be significant. false Zhangyu Cheng annotation2484237 1 pRM range2484237 1 338 373 annotation2484235 1 terminator range2484235 1 202 330 annotation2484238 1 RBS core range2484238 1 374 386 annotation2484234 1 Cro range2484234 1 1 201 annotation2484240 1 LVAssrAtag range2484240 1 1107 1148 annotation2484236 1 scar range2484236 1 331 337 annotation2484239 1 GFP range2484239 1 393 1106 BBa_K2036010_sequence 1 atgaaagcgtattgggactctttaaccaaagaacagcagggcgagttggccggaaaagttggctcaacacctggctacttacggctggttttcaatggctataaaaaagccagttttgtgctggctaaaaaacttgagcaatacacatcaggtgcaattacgaaatctgacttaagaccggatatctatccgaaagattagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctagagttagatatttatcccttgcggtgatagatttaacgtaaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagctgcaaacgacgaaaactacgctttagtagcttaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z