LOCUS       BBa_K2036014            1662 bp    DNA     linear       17-May-2024
DEFINITION  RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag
ACCESSION   BBa_K2036014
VERSION     BBa_K2036014.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=RBS
     misc_feature    13..18
                     /label=spacer sequence
     CDS             1..183
                     /label=BBa_K2036006
     RBS             192..203
                     /label=RBS
     misc_feature    204..209
                     /label=spacer sequence
     CDS             192..374
                     /label=BBa_K2036006
     RBS             1..12
                     /label=RBS
     misc_feature    13..18
                     /label=spacer sequence
     misc_feature    1..183
                     /label=BBa_K2036006
     RBS             192..203
                     /label=RBS
     misc_feature    204..209
                     /label=spacer sequence
     misc_feature    192..374
                     /label=BBa_K2036006
     misc_feature    1..374
                     /label=BBa_K2036008
     RBS             383..394
                     /label=RBS core
     CDS             401..694
                     /label=CII
     stem_loop       695..823
                     /label=terminator
     misc_feature    383..823
                     /label=BBa_K2036012
     promoter        832..883
                     /label=pRE
     RBS             884..895
                     /label=RBS core
     CDS             907..1620
                     /label=GFP
     misc_feature    902..906
                     /label=scar
     misc_feature    1621..1662
                     /label=LVAssrAtag
     misc_feature    832..1662
                     /label=BBa_K2036011
     misc_feature    383..1662
                     /label=BBa_K2036013
ORIGIN
        1 aaagaggaga aatacacgat gcaatatgcc attgcagggt ggcctgttgc tggctgccct
       61 tccgaatctt tacttgaacg aatcacccgt aaattacgtg acggatggaa acgccttatc
      121 gacatactta atcagccagg agtcccaaag aatggatcaa acacttatgg ctatccagac
      181 taatactaga gaaagaggag aaatacacga tgcaatatgc cattgcaggg tggcctgttg
      241 ctggctgccc ttccgaatct ttacttgaac gaatcacccg taaattacgt gacggatgga
      301 aacgccttat cgacatactt aatcagccag gagtcccaaa gaatggatca aacacttatg
      361 gctatccaga ctaatactag agaaagagga gaaatacacg atggttcgtg caaacaaacg
      421 caacgaggct ctacgaatcg agagtgcgtt gcttaacaaa atcgcaatgc ttggaactga
      481 gaagacagcg gaagctgtgg gcgttgataa gtcgcagatc agcaggtgga agagggactg
      541 gattccaaag ttctcaatgc tgcttgctgt tcttgaatgg ggggtcgttg acgacgacat
      601 ggctcgattg gcgcgacaag ttgctgcgat tctcaccaat aaaaaacgcc cggcggcaac
      661 cgagcgttct gaacaaatcc agatggagtt ctgaccaggc atcaaataaa acgaaaggct
      721 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      781 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagcctcg
      841 ttgcgtttgt ttgcacgaac catatgtaag tatttcctta gataaagagg agaaatacac
      901 gactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      961 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
     1021 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
     1081 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
     1141 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
     1201 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
     1261 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
     1321 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
     1381 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
     1441 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     1501 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     1561 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
     1621 gctgcaaacg acgaaaacta cgctttagta gcttaataat aa
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