BBa_K2036013 1 BBa_K2036013 RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag 2016-09-18T11:00:00Z 2016-09-24T05:05:39Z CII and pRE are a part of bactriaphage lambda operon. RBS-CII-TT-pRE-RBS-GFP-LVAtag, it is a GFP regulationary circuits. When CII is expressed it can further activate GFP and forming a Switching Lag-times. false false _2503_ 28503 25901 9 false When CII is expressed it can further activate GFP and forming a Switching Lag-times. And in HUST-China 2016 designed circuit,this composite is constructed to test CII and pRE interaction. false Zhangyu Cheng component2484204 1 BBa_K2036012 component2484205 1 BBa_K2036011 annotation2484205 1 BBa_K2036011 range2484205 1 450 1280 annotation2484204 1 BBa_K2036012 range2484204 1 1 441 BBa_K2036014 1 BBa_K2036014 RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag 2016-09-18T11:00:00Z 2016-09-24T05:06:04Z bacteriaphage lambda RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAtag false false _2503_ 28503 25901 9 false add a promoter frontier false Zhangyu Cheng component2484212 1 BBa_K2036008 component2484207 1 BBa_K2036006 component2484210 1 BBa_K2036006 component2484215 1 BBa_K2036013 annotation2484210 1 BBa_K2036006 range2484210 1 192 374 annotation2484207 1 BBa_K2036006 range2484207 1 1 183 annotation2484212 1 BBa_K2036008 range2484212 1 1 374 annotation2484215 1 BBa_K2036013 range2484215 1 383 1662 BBa_K2036011 1 BBa_K2036011 pRE-GFP-LVAssrAtag 2016-09-18T11:00:00Z 2016-09-24T05:02:49Z pRE comes from bacteriaphage lambda and GFP-LVAtag are standard part from Rgistry pRE-GFP-LVAtag, it is a GFP generator,and the production of GFP will be activated by CII and CIII. false false _2503_ 28503 25901 9 false One should put CII as an activator of pRE when constructing gene circuit. false Zhangyu Cheng annotation2484245 1 LVAssrAtag range2484245 1 790 831 annotation2484241 1 pRE range2484241 1 1 52 annotation2484242 1 RBS core range2484242 1 53 64 annotation2484244 1 scar range2484244 1 71 75 annotation2484243 1 GFP range2484243 1 76 789 BBa_K2036012 1 BBa_K2036012 RBS-CII-TT 2016-09-18T11:00:00Z 2016-09-20T01:36:08Z come form bacteriaphage lambda. RBS-CII-TT, CII is a regulatory gene derived from bacteriaphage lambda. It is an activate pRE's expression. And it can be normally degrade by Ftsh in E.coli. false false _2503_ 25901 25901 9 false CII is an transcriptional activaor. One should add a related promoter downstream of CII. false Zhangyu Cheng annotation2484264 1 RBS core range2484264 1 1 12 annotation2484266 1 terminator range2484266 1 313 441 annotation2484265 1 CII range2484265 1 19 312 BBa_K2036006 1 BBa_K2036006 RBS-CIII 2016-09-18T11:00:00Z 2016-09-28T01:20:43Z come from bacteriaphage lambda CIII is a inhibitor of Ftsh,we add a RBS to provide more possibilities to users to create more complex circuit. false false _2503_ 25901 25901 9 false you can add this part behind a promoter region or a coding region for tandom expression false Zhangyu Cheng annotation2484150 1 RBS range2484150 1 1 12 annotation2484151 1 spacer sequence range2484151 1 13 18 BBa_K2036008 1 BBa_K2036008 RBS-CIII-RBS-CIII,tandem expression 2016-09-18T11:00:00Z 2016-10-24T11:09:59Z come from bacteriaphage lambda RBS-CIII-RBS-CIII,as Ftsh normally exists in E.coli to degrade CII, CIII functions as a inhibitor of Ftsh, and tandom expression of CIII,may enhance the effect on Ftsh false false _2503_ 28503 25901 9 false According to the Registry, prefix of other sequence are supposed to be "GAATTCGCGGCCGCTTCTAGAG" , so the scar produced by 3A assembly should be "TACTAGAG" false Zhangyu Cheng annotation2484159 1 BBa_K2036006 range2484159 1 1 183 annotation2484158 1 spacer sequence range2484158 1 13 18 annotation2484160 1 RBS range2484160 1 192 203 annotation2484161 1 spacer sequence range2484161 1 204 209 annotation2484162 1 BBa_K2036006 range2484162 1 192 374 annotation2484157 1 RBS range2484157 1 1 12 BBa_K2036012_sequence 1 aaagaggagaaatacacgatggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2036008_sequence 1 aaagaggagaaatacacgatgcaatatgccattgcagggtggcctgttgctggctgcccttccgaatctttacttgaacgaatcacccgtaaattacgtgacggatggaaacgccttatcgacatacttaatcagccaggagtcccaaagaatggatcaaacacttatggctatccagactaatactagagaaagaggagaaatacacgatgcaatatgccattgcagggtggcctgttgctggctgcccttccgaatctttacttgaacgaatcacccgtaaattacgtgacggatggaaacgccttatcgacatacttaatcagccaggagtcccaaagaatggatcaaacacttatggctatccagactaa BBa_K2036006_sequence 1 aaagaggagaaatacacgatgcaatatgccattgcagggtggcctgttgctggctgcccttccgaatctttacttgaacgaatcacccgtaaattacgtgacggatggaaacgccttatcgacatacttaatcagccaggagtcccaaagaatggatcaaacacttatggctatccagactaa BBa_K2036014_sequence 1 aaagaggagaaatacacgatgcaatatgccattgcagggtggcctgttgctggctgcccttccgaatctttacttgaacgaatcacccgtaaattacgtgacggatggaaacgccttatcgacatacttaatcagccaggagtcccaaagaatggatcaaacacttatggctatccagactaatactagagaaagaggagaaatacacgatgcaatatgccattgcagggtggcctgttgctggctgcccttccgaatctttacttgaacgaatcacccgtaaattacgtgacggatggaaacgccttatcgacatacttaatcagccaggagtcccaaagaatggatcaaacacttatggctatccagactaatactagagaaagaggagaaatacacgatggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttccttagataaagaggagaaatacacgactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagctgcaaacgacgaaaactacgctttagtagcttaataataa BBa_K2036011_sequence 1 agagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttccttagataaagaggagaaatacacgactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagctgcaaacgacgaaaactacgctttagtagcttaataataa BBa_K2036013_sequence 1 aaagaggagaaatacacgatggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttccttagataaagaggagaaatacacgactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagctgcaaacgacgaaaactacgctttagtagcttaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z