LOCUS       BBa_K2036015            1882 bp    DNA     linear       31-Dec-2025
DEFINITION  RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag
ACCESSION   BBa_K2036015
VERSION     BBa_K2036015.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=RBS core sequence
     misc_feature    19..21
                     /label=Start codon
     CDS             1..294
                     /label=BBa_K2036000
     RBS             301..312
                     /label=RBS core sequence
     misc_feature    319..321
                     /label=Start codon
     CDS             301..594
                     /label=BBa_K2036000
     misc_feature    1..594
                     /label=BBa_K2036007
     RBS             603..614
                     /label=RBS core
     CDS             621..914
                     /label=CII
     stem_loop       915..1043
                     /label=terminator
     misc_feature    603..1043
                     /label=BBa_K2036012
     promoter        1052..1103
                     /label=pRE
     RBS             1104..1115
                     /label=RBS core
     CDS             1127..1840
                     /label=GFP
     misc_feature    1122..1126
                     /label=scar
     misc_feature    1841..1882
                     /label=LVAssrAtag
     misc_feature    1052..1882
                     /label=BBa_K2036011
     misc_feature    603..1882
                     /label=BBa_K2036013
ORIGIN
        1 atggttcgtg caaacaaacg caacgaggct ctacgaatcg agagtgcgtt gcttaacaaa
       61 atcgcaatgc ttggaactga gaagacagcg gaagctgtgg gcgttgataa gtcgcagatc
      121 agcaggtgga agagggactg gattccaaag ttctcaatgc tgcttgctgt tcttgaatgg
      181 ggggtcgttg acgacgacat ggctcgattg gcgcgacaag ttgctgcgat tctcaccaat
      241 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgatactag
      301 atggttcgtg caaacaaacg caacgaggct ctacgaatcg agagtgcgtt gcttaacaaa
      361 atcgcaatgc ttggaactga gaagacagcg gaagctgtgg gcgttgataa gtcgcagatc
      421 agcaggtgga agagggactg gattccaaag ttctcaatgc tgcttgctgt tcttgaatgg
      481 ggggtcgttg acgacgacat ggctcgattg gcgcgacaag ttgctgcgat tctcaccaat
      541 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgatactag
      601 agaaagagga gaaatacacg atggttcgtg caaacaaacg caacgaggct ctacgaatcg
      661 agagtgcgtt gcttaacaaa atcgcaatgc ttggaactga gaagacagcg gaagctgtgg
      721 gcgttgataa gtcgcagatc agcaggtgga agagggactg gattccaaag ttctcaatgc
      781 tgcttgctgt tcttgaatgg ggggtcgttg acgacgacat ggctcgattg gcgcgacaag
      841 ttgctgcgat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      901 agatggagtt ctgaccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      961 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1021 gggtgggcct ttctgcgttt atatactaga gagagcctcg ttgcgtttgt ttgcacgaac
     1081 catatgtaag tatttcctta gataaagagg agaaatacac gactagatgc gtaaaggaga
     1141 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
     1201 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
     1261 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
     1321 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
     1381 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
     1441 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
     1501 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
     1561 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
     1621 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
     1681 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
     1741 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
     1801 agctgctggg attacacatg gcatggatga actatacaaa gctgcaaacg acgaaaacta
     1861 cgctttagta gcttaataat aa
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