LOCUS       BBa_K2036020            2862 bp    DNA     linear       30-Dec-2025
DEFINITION  RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag
ACCESSION   BBa_K2036020
VERSION     BBa_K2036020.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=RBS core
     misc_feature    13..18
                     /label=Interval sequence
     CDS             19..732
                     /label=CI
     stem_loop       733..861
                     /label=stem_loop
     promoter        862..955
                     /label=pR
     RBS             956..967
                     /label=RBS core
     misc_feature    968..973
                     /label=Interval sequence
     CDS             974..1138
                     /label=CIII
     misc_feature    1139..1146
                     /label=scar
     RBS             1147..1158
                     /label=RBS-core
     misc_feature    1159..1164
                     /label=Interval sequence
     CDS             1165..2040
                     /label=RFP-LAAssrAtag
     misc_feature    2041..2048
                     /label=scar
     promoter        2049..2085
                     /label=pRM
     RBS             2086..2097
                     /label=RBS core
     misc_feature    2098..2103
                     /label=Interval sequence
     CDS             2104..2862
                     /label=GFP-LVAssrAtag
ORIGIN
        1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
       61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
      241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
      481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
      541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
      601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
      661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
      721 acgtttggct gaccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      781 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      841 gtgggccttt ctgcgtttat aaaatctatc accgcaaggg ataaatatct aacaccgtgc
      901 gtgttgacta ttttacctct ggcggtgata atggttgcat gtactaagga ggttgaaaga
      961 ggagaaatac acgatgcaat atgccattgc agggtggcct gttgctggct gcccttccga
     1021 atctttactt gaacgaatca cccgtaaatt acgtgacgga tggaaacgcc ttatcgacat
     1081 acttaatcag ccaggagtcc caaagaatgg atcaaacact tatggctatc cagactaata
     1141 ctagagaaag aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg
     1201 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa
     1261 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt
     1321 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac
     1381 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa
     1441 tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc
     1501 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac
     1561 ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg
     1621 gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac
     1681 tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct
     1741 tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa
     1801 cagtacgaac gtgctgaagg tcgtcactcc accggtgctg ctgcaaacga cgaaaactac
     1861 gctctggctg cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc
     1921 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1981 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     2041 tactagagtt agatatttat cccttgcggt gatagattta acgtaaaaga ggagaaatac
     2101 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     2161 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
     2221 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
     2281 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
     2341 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
     2401 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
     2461 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
     2521 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
     2581 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
     2641 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     2701 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     2761 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaagct
     2821 gcaaacgacg aaaactacgc tttagtagct taataataat aa
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