LOCUS       BBa_K2036023            1747 bp    DNA     linear       26-Apr-2024
DEFINITION  RBS-iLDH-TT-pRM-RBS-beta-galactosidase
ACCESSION   BBa_K2036023
VERSION     BBa_K2036023.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS core
     misc_feature    16..21
                     /label=Interval sequence
     CDS             22..1212
                     /label=iLDH
     stem_loop       1213..1341
                     /label=Terminater
     CDS             1342..1747
                     /label=beta-galactosidase
     misc_feature    945..945
                     /label=s_mutation
     misc_feature    1450..1450
                     /label=s_mutation
     misc_feature    1465..1465
                     /label=s_mutation
     misc_feature    1489..1489
                     /label=s_mutation
ORIGIN
        1 attaaagagg agaaatacta gatgattatt tccgcagcca gcgattatcg cgccgcagcg
       61 caacgcattc tgccgccgtt cctgttccac tatatggatg gtggtgcata ttctgaatac
      121 acgctgcgcc gcaacgtgga agatttgtca gaagtggcgc tgcgccagcg tattctgaaa
      181 aacatgtccg acttaagcct ggaaacgacg ctgtttaatg agaaattgtc gatgccggtg
      241 gcactggctc cggtgggttt gtgtggcatg tatgcgcgtc gtggcgaagt tcaggcagcc
      301 aaagcggcgg acgcgcatgg tattccgttt actctctcga cggtttccgt ttgcccgatt
      361 gaagaagtcg cgccagccat caagcgccca atgtggttcc agctttatgt actgcgcgat
      421 cgcggcttta tgcgtaacgc gctggagcga gcaaaagcag cgggttgttc gacgctggtt
      481 ttcaccgtgg atatgccgac accgggcgca cgctaccgtg atgcgcattc aggtatgagc
      541 ggcccgaacg cggcaatgcg ccgctacttg caagcggtga cacatccgca atgggcgtgg
      601 gatgtgggcc tgaacggtcg tccacatgat ttaggtaata tctcagctta tctcggcaaa
      661 ccgaccggac tggaagatta catcggctgg ctggggaata acttcgatcc gtccatctca
      721 tggaaagacc ttgaatggat ccgcgatttc tgggatggcc cgatggtgat caaagggatc
      781 ctcgatccgg aagatgcgcg cgatgcagta cgttttggtg ctgatggaat tgtggtttct
      841 aaccacggtg gccgccagct ggacggtgta ctctcttccg cccgtgcact gcctgctatt
      901 gcagatgcgg tgaaaggtga tatagccatt ctggcggata gcggtattcg taacgggctt
      961 gatgtcgtgc gtatgattgc gctcggtgcc gacaccgtac tgctgggtcg tgctttcttg
     1021 tatgcgctgg caacagcggg ccaggcgggt gtagctaacc tgctaaatct gatcgaaaaa
     1081 gagatgaaag tggcgatgac gctgactggc gcgaaatcga tcagcgaaat tacgcaagat
     1141 tcgctggtgc aggggctggg taaagagttg cctgcggcac tggctcccat ggcgaaaggg
     1201 aatgcggcat agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1261 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1321 gtgggccttt ctgcgtttat aaaatagtca acacgcacgg tgttagatat ttatcccttg
     1381 cggtgataga tttaacgtaa ttaaagagga gaaatactag atgaccatga ttacgccaag
     1441 cttgcatgcg tgcaggtcga ctcttgagga tccccgggta ccgagctcca attcactggc
     1501 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     1561 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     1621 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     1681 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     1741 gcattaa
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