LOCUS BBa_K2036024 4222 bp DNA linear 26-Nov-2024 DEFINITION placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-T T-pRM-RBS-beta-galactosidase ACCESSION BBa_K2036024 VERSION BBa_K2036024.1 FEATURES Location/Qualifiers misc_feature 1..245 /label=BBa_M36138 misc_feature 246..251 /label=Interval sequence promoter 252..303 /label=pRE RBS 304..318 /label=RBS core CDS 319..525 /label=Cro RBS 526..537 /label=RBS core misc_feature 538..543 /label=Interval sequence CDS 544..837 /label=CII stem_loop 838..966 /label=TT misc_feature 1..966 /label=BBa_K2036021 promoter 975..1347 /label=patp2(RBS included) CDS 1348..2061 /label=CI stem_loop 2062..2190 /label=Terminator promoter 2191..2284 /label=pR RBS 2285..2302 /label=RBS CDS 2303..2467 /label=CIII misc_feature 975..2467 /label=BBa_K2036022 RBS 2476..2490 /label=RBS core misc_feature 2491..2496 /label=Interval sequence CDS 2497..3687 /label=iLDH stem_loop 3688..3816 /label=Terminater CDS 3817..4222 /label=beta-galactosidase misc_feature 3420..3420 /label=s_mutation misc_feature 3925..3925 /label=s_mutation misc_feature 3940..3940 /label=s_mutation misc_feature 3964..3964 /label=s_mutation misc_feature 2476..4222 /label=BBa_K2036023 ORIGIN 1 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc 61 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 121 tgagcgcaac gcaattccac gctgtatccg atgtcagtag gcaccccagg ctttacactt 181 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 241 cagcttacac gagagcctcg ttgcgtttgt ttgcacgaac catatgtaag tatttcctta 301 gatattaaag aggagaaata cacgatgaaa gcgtattggg actctttaac caaagaacag 361 cagggcgagt tggccggaaa agttggctca acacctggct acttacggct ggttttcaat 421 ggctataaaa aagccagttt tgtgctggct aaaaaacttg agcaatacac atcaggtgca 481 attacgaaat ctgacttaag accggatatc tatccgaaag attagaaaga ggagaaatac 541 acgatggttc gtgcaaacaa acgcaacgag gctctacgaa tcgagagtgc gttgcttaac 601 aaaatcgcaa tgcttggaac tgagaagaca gcggaagctg tgggcgttga taagtcgcag 661 atcagcaggt ggaagaggga ctggattcca aagttctcaa tgctgcttgc tgttcttgaa 721 tggggggtcg ttgacgacga catggctcga ttggcgcgac aagttgctgc gattctcacc 781 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgacca 841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 961 tttatatact agagagtgtc cgtgctggga aactggcggg gaacttttag agataaccct 1021 ccgaattgct ggcaaatttc tgatgaaatt tctccgcgaa gcccacatga actacccccg 1081 tttaccctca aaataagccc tgtgacacac ataacacccc ctaatcgtac ccgctcacac 1141 gctattttcg aggtgtggtt cgccttcgga aacgaatgcc cccgccccac ttggataaaa 1201 gacgttaaca cctgttagtc tataacgcgg gttgaaccga gaaacccctc aaggcagcag 1261 acaatagccg caaggggttt tgcggagcac gtcccctgtg atcgttgcgc tgatgtgcga 1321 cggagtccga aagaggagaa atactagatg agcacaaaaa agaaaccatt aacacaagag 1381 cagcttgagg acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc 1441 ttatcccagg aatctgtcgc agacaagatg gggatggggc agtcaggcgt tggtgcttta 1501 tttaatggca tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa 1561 gttagcgttg aagaatttag cccttcaatc gccagagaaa tctacgagat gtatgaagcg 1621 gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc tcatgttcag 1681 gcagggatgt tctcacctga gcttagaacc tttaccaaag gtgatgcgga gagatgggta 1741 agcacaacca aaaaagccag tgattctgca ttctggcttg aggttgaagg taattccatg 1801 accgcaccaa caggctccaa gccaagcttt cctgacggaa tgttaattct cgttgaccct 1861 gagcaggctg ttgagccagg tgatttctgc atagccagac ttgggggtga tgagtttacc 1921 ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa cccacagtac 1981 ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc tagtcagtgg 2041 cctgaagaga cgtttggctg accaggcatc aaataaaacg aaaggctcag tcgaaagact 2101 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 2161 caccttcggg tgggcctttc tgcgtttata aaatctatca ccgcaaggga taaatatcta 2221 acaccgtgcg tgttgactat tttacctctg gcggtgataa tggttgcatg tactaaggag 2281 gttgaaagag gagaaataca cgatgcaata tgccattgca gggtggcctg ttgctggctg 2341 cccttccgaa tctttacttg aacgaatcac ccgtaaatta cgtgacggat ggaaacgcct 2401 tatcgacata cttaatcagc caggagtccc aaagaatgga tcaaacactt atggctatcc 2461 agactaatac tagagattaa agaggagaaa tactagatga ttatttccgc agccagcgat 2521 tatcgcgccg cagcgcaacg cattctgccg ccgttcctgt tccactatat ggatggtggt 2581 gcatattctg aatacacgct gcgccgcaac gtggaagatt tgtcagaagt ggcgctgcgc 2641 cagcgtattc tgaaaaacat gtccgactta agcctggaaa cgacgctgtt taatgagaaa 2701 ttgtcgatgc cggtggcact ggctccggtg ggtttgtgtg gcatgtatgc gcgtcgtggc 2761 gaagttcagg cagccaaagc ggcggacgcg catggtattc cgtttactct ctcgacggtt 2821 tccgtttgcc cgattgaaga agtcgcgcca gccatcaagc gcccaatgtg gttccagctt 2881 tatgtactgc gcgatcgcgg ctttatgcgt aacgcgctgg agcgagcaaa agcagcgggt 2941 tgttcgacgc tggttttcac cgtggatatg ccgacaccgg gcgcacgcta ccgtgatgcg 3001 cattcaggta tgagcggccc gaacgcggca atgcgccgct acttgcaagc ggtgacacat 3061 ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccac atgatttagg taatatctca 3121 gcttatctcg gcaaaccgac cggactggaa gattacatcg gctggctggg gaataacttc 3181 gatccgtcca tctcatggaa agaccttgaa tggatccgcg atttctggga tggcccgatg 3241 gtgatcaaag ggatcctcga tccggaagat gcgcgcgatg cagtacgttt tggtgctgat 3301 ggaattgtgg tttctaacca cggtggccgc cagctggacg gtgtactctc ttccgcccgt 3361 gcactgcctg ctattgcaga tgcggtgaaa ggtgatatag ccattctggc ggatagcggt 3421 attcgtaacg ggcttgatgt cgtgcgtatg attgcgctcg gtgccgacac cgtactgctg 3481 ggtcgtgctt tcttgtatgc gctggcaaca gcgggccagg cgggtgtagc taacctgcta 3541 aatctgatcg aaaaagagat gaaagtggcg atgacgctga ctggcgcgaa atcgatcagc 3601 gaaattacgc aagattcgct ggtgcagggg ctgggtaaag agttgcctgc ggcactggct 3661 cccatggcga aagggaatgc ggcatagcca ggcatcaaat aaaacgaaag gctcagtcga 3721 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 3781 ctggctcacc ttcgggtggg cctttctgcg tttataaaat agtcaacacg cacggtgtta 3841 gatatttatc ccttgcggtg atagatttaa cgtaattaaa gaggagaaat actagatgac 3901 catgattacg ccaagcttgc atgcgtgcag gtcgactctt gaggatcccc gggtaccgag 3961 ctccaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 4021 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 4081 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta 4141 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac 4201 gcgccctgta gcggcgcatt aa //