##gff-version 3
##sequence-region BBa_K2036024 1 4222
BBa_K2036024	.	engineered_region	975	2467	.	+	0	ID=BBa_K2036022;Name=BBa_K2036022
BBa_K2036024	.	stem_loop	2062	2190	.	+	0	ID=annotation2485651;Name=Terminator;Parent=BBa_K2036022
BBa_K2036024	.	promoter	975	1347	.	+	0	ID=annotation2484694;Name=patp2(RBS included);Parent=BBa_K2036022
BBa_K2036024	.	promoter	2191	2284	.	+	0	ID=annotation2485652;Name=pR;Parent=BBa_K2036022
BBa_K2036024	.	CDS	2303	2467	.	+	0	ID=annotation2485654;Name=CIII;Parent=BBa_K2036022
BBa_K2036024	.	CDS	1348	2061	.	+	0	ID=annotation2485650;Name=CI;Parent=BBa_K2036022
BBa_K2036024	.	ribosome_entry_site	2285	2302	.	+	0	ID=annotation2485653;Name=RBS;Parent=BBa_K2036022
BBa_K2036024	.	engineered_region	1	966	.	+	0	ID=BBa_K2036021;Name=BBa_K2036021
BBa_K2036024	.	sequence_feature	538	543	.	+	0	ID=annotation2484691;Name=Interval sequence;Parent=BBa_K2036021
BBa_K2036024	.	CDS	544	837	.	+	0	ID=annotation2484692;Name=CII;Parent=BBa_K2036021
BBa_K2036024	.	stem_loop	838	966	.	+	0	ID=annotation2484693;Name=TT;Parent=BBa_K2036021
BBa_K2036024	.	engineered_region	1	245	.	+	0	ID=annotation2484685;Name=BBa_M36138;Parent=BBa_K2036021
BBa_K2036024	.	ribosome_entry_site	526	537	.	+	0	ID=annotation2484690;Name=RBS core;Parent=BBa_K2036021
BBa_K2036024	.	ribosome_entry_site	304	318	.	+	0	ID=annotation2484688;Name=RBS core;Parent=BBa_K2036021
BBa_K2036024	.	sequence_feature	246	251	.	+	0	ID=annotation2484686;Name=Interval sequence;Parent=BBa_K2036021
BBa_K2036024	.	promoter	252	303	.	+	0	ID=annotation2484687;Name=pRE;Parent=BBa_K2036021
BBa_K2036024	.	CDS	319	525	.	+	0	ID=annotation2484689;Name=Cro;Parent=BBa_K2036021
BBa_K2036024	.	engineered_region	2476	4222	.	+	0	ID=BBa_K2036023;Name=BBa_K2036023
BBa_K2036024	.	CDS	2497	3687	.	+	0	ID=annotation2484697;Name=iLDH;Parent=BBa_K2036023
BBa_K2036024	.	sequence_feature	2491	2496	.	+	0	ID=annotation2484696;Name=Interval sequence;Parent=BBa_K2036023
BBa_K2036024	.	point_mutation	3925	3925	.	+	0	ID=annotation2484701;Name=s_mutation;Parent=BBa_K2036023
BBa_K2036024	.	point_mutation	3964	3964	.	+	0	ID=annotation2484703;Name=s_mutation;Parent=BBa_K2036023
BBa_K2036024	.	ribosome_entry_site	2476	2490	.	+	0	ID=annotation2484695;Name=RBS core;Parent=BBa_K2036023
BBa_K2036024	.	stem_loop	3688	3816	.	+	0	ID=annotation2484698;Name=Terminater;Parent=BBa_K2036023
BBa_K2036024	.	point_mutation	3940	3940	.	+	0	ID=annotation2484702;Name=s_mutation;Parent=BBa_K2036023
BBa_K2036024	.	point_mutation	3420	3420	.	+	0	ID=annotation2484700;Name=s_mutation;Parent=BBa_K2036023
BBa_K2036024	.	CDS	3817	4222	.	+	0	ID=annotation2484699;Name=beta-galactosidase;Parent=BBa_K2036023
>BBa_K2036024
gtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattccacgctgtatccgatgtcagt
aggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacagg
aaacagcttacacgagagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttccttagatattaaagaggaga
aatacacgatgaaagcgtattgggactctttaaccaaagaacagcagggcgagttggccggaaaagttggctcaacacc
tggctacttacggctggttttcaatggctataaaaaagccagttttgtgctggctaaaaaacttgagcaatacacatca
ggtgcaattacgaaatctgacttaagaccggatatctatccgaaagattagaaagaggagaaatacacgatggttcgtg
caaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagc
ggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgct
gttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaac
gcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagagtgtccgtgctgggaaactggcggggaacttttagagataaccctccgaatt
gctggcaaatttctgatgaaatttctccgcgaagcccacatgaactacccccgtttaccctcaaaataagccctgtgac
acacataacaccccctaatcgtacccgctcacacgctattttcgaggtgtggttcgccttcggaaacgaatgcccccgc
cccacttggataaaagacgttaacacctgttagtctataacgcgggttgaaccgagaaacccctcaaggcagcagacaa
tagccgcaaggggttttgcggagcacgtcccctgtgatcgttgcgctgatgtgcgacggagtccgaaagaggagaaata
ctagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaa
aagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttat
ttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttag
cccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtac
cctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatggg
taagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctc
caagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagcc
agacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccac
agtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtt
tggctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataaaatctatcaccgcaagggata
aatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcatgtactaaggaggttgaaagagg
agaaatacacgatgcaatatgccattgcagggtggcctgttgctggctgcccttccgaatctttacttgaacgaatcac
ccgtaaattacgtgacggatggaaacgccttatcgacatacttaatcagccaggagtcccaaagaatggatcaaacact
tatggctatccagactaatactagagattaaagaggagaaatactagatgattatttccgcagccagcgattatcgcgc
cgcagcgcaacgcattctgccgccgttcctgttccactatatggatggtggtgcatattctgaatacacgctgcgccgc
aacgtggaagatttgtcagaagtggcgctgcgccagcgtattctgaaaaacatgtccgacttaagcctggaaacgacgc
tgtttaatgagaaattgtcgatgccggtggcactggctccggtgggtttgtgtggcatgtatgcgcgtcgtggcgaagt
tcaggcagccaaagcggcggacgcgcatggtattccgtttactctctcgacggtttccgtttgcccgattgaagaagtc
gcgccagccatcaagcgcccaatgtggttccagctttatgtactgcgcgatcgcggctttatgcgtaacgcgctggagc
gagcaaaagcagcgggttgttcgacgctggttttcaccgtggatatgccgacaccgggcgcacgctaccgtgatgcgca
ttcaggtatgagcggcccgaacgcggcaatgcgccgctacttgcaagcggtgacacatccgcaatgggcgtgggatgtg
ggcctgaacggtcgtccacatgatttaggtaatatctcagcttatctcggcaaaccgaccggactggaagattacatcg
gctggctggggaataacttcgatccgtccatctcatggaaagaccttgaatggatccgcgatttctgggatggcccgat
ggtgatcaaagggatcctcgatccggaagatgcgcgcgatgcagtacgttttggtgctgatggaattgtggtttctaac
cacggtggccgccagctggacggtgtactctcttccgcccgtgcactgcctgctattgcagatgcggtgaaaggtgata
tagccattctggcggatagcggtattcgtaacgggcttgatgtcgtgcgtatgattgcgctcggtgccgacaccgtact
gctgggtcgtgctttcttgtatgcgctggcaacagcgggccaggcgggtgtagctaacctgctaaatctgatcgaaaaa
gagatgaaagtggcgatgacgctgactggcgcgaaatcgatcagcgaaattacgcaagattcgctggtgcaggggctgg
gtaaagagttgcctgcggcactggctcccatggcgaaagggaatgcggcatagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttataaaatagtcaacacgcacggtgttagatatttatcccttgcggtgatagatttaac
gtaattaaagaggagaaatactagatgaccatgattacgccaagcttgcatgcgtgcaggtcgactcttgaggatcccc
gggtaccgagctccaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcg
ccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgc
agcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaa
gcaaccatagtacgcgccctgtagcggcgcattaa