BBa_K2036024 1 BBa_K2036024 placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase 2016-09-19T11:00:00Z 2016-09-24T02:27:51Z iLDH are from the Registry and beta-galactosidase coding gene are referred to pUC119 commerial vector and synthesised by IDT. RBS-iLDH-TT-pRM-RBS-beta-galactosidase,iLDH are lactic dehydrogenase,while beta-galactosidase can transfer lactose into monosaccharide. false false _2503_ 28503 25901 9 false beta-galactosidase coding gene are alpha domain of laz,it functions only in E.coli strain which contains lacZ operon. false Zhangyu Cheng component2484724 1 BBa_K2036023 component2484713 1 BBa_K2036021 component2484714 1 BBa_K2036022 annotation2484724 1 BBa_K2036023 range2484724 1 2476 4222 annotation2484714 1 BBa_K2036022 range2484714 1 975 2467 annotation2484713 1 BBa_K2036021 range2484713 1 1 966 BBa_K2036021 1 BBa_K2036021 placm-pRE-RBS-Cro-RBS-CII-TT 2016-09-19T11:00:00Z 2016-09-24T01:11:15Z The circuit are redesign of bacteriaphage lambda operon. pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAtag-TT-pRM-RBS-GFP-LVAtag false false _2503_ 28503 25901 9 Not in stock false It is a tri-stable system with a certain threshold and it functions as a basic gene expression toolkit for two input and three related output. false Zhangyu Cheng annotation2484685 1 BBa_M36138 range2484685 1 1 245 annotation2484691 1 Interval sequence range2484691 1 538 543 annotation2484686 1 Interval sequence range2484686 1 246 251 annotation2484687 1 pRE range2484687 1 252 303 annotation2484690 1 RBS core range2484690 1 526 537 annotation2484693 1 TT range2484693 1 838 966 annotation2484689 1 Cro range2484689 1 319 525 annotation2484688 1 RBS core range2484688 1 304 318 annotation2484692 1 CII range2484692 1 544 837 BBa_K2036023 1 BBa_K2036023 RBS-iLDH-TT-pRM-RBS-beta-galactosidase 2016-09-19T11:00:00Z 2016-09-24T02:03:17Z patp2 are from Corynebacterium glutamicum ATCC 13032, CI, CIII and pR are all from bacteriaphage lambda. patp2-RBS-CI-TT-pR-RBS-CIII false false _2503_ 28503 25901 9 false patp2 are active within pH7~9, so CI will be produced to inhibit pR thus we can regulate CIII's expression by PH level. false Zhangyu Cheng annotation2484701 1 s_mutation range2484701 1 1450 1450 annotation2484696 1 Interval sequence range2484696 1 16 21 annotation2484698 1 Terminater range2484698 1 1213 1341 annotation2484703 1 s_mutation range2484703 1 1489 1489 annotation2484702 1 s_mutation range2484702 1 1465 1465 annotation2484699 1 beta-galactosidase range2484699 1 1342 1747 annotation2484695 1 RBS core range2484695 1 1 15 annotation2484700 1 s_mutation range2484700 1 945 945 annotation2484697 1 iLDH range2484697 1 22 1212 BBa_K2036022 1 BBa_K2036022 patp2-RBS-CI-TT-pR-RBS-CIII 2016-09-19T11:00:00Z 2016-09-24T01:57:00Z placm is a mutant lac promoter which has been deleted a illegal restriction site. pRE, Cro, CII are from bacteriaphage lambda. placm-pRE-RBS-Cro-RBS-CII-TT false false _2503_ 28503 25901 9 false With this two pormoter design, lactose an induce downstream gene's expression,and CII protein can form a positive feedback by activate pRE so, the circuit can fastly reach a stable state for Cro expression. false Zhangyu Cheng annotation2485653 1 RBS range2485653 1 1311 1328 annotation2485650 1 CI range2485650 1 374 1087 annotation2485654 1 CIII range2485654 1 1329 1493 annotation2484694 1 patp2(RBS included) range2484694 1 1 373 annotation2485651 1 Terminator range2485651 1 1088 1216 annotation2485652 1 pR range2485652 1 1217 1310 BBa_K2036021_sequence 1 gtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattccacgctgtatccgatgtcagtaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagcttacacgagagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttccttagatattaaagaggagaaatacacgatgaaagcgtattgggactctttaaccaaagaacagcagggcgagttggccggaaaagttggctcaacacctggctacttacggctggttttcaatggctataaaaaagccagttttgtgctggctaaaaaacttgagcaatacacatcaggtgcaattacgaaatctgacttaagaccggatatctatccgaaagattagaaagaggagaaatacacgatggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2036024_sequence 1 gtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattccacgctgtatccgatgtcagtaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagcttacacgagagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttccttagatattaaagaggagaaatacacgatgaaagcgtattgggactctttaaccaaagaacagcagggcgagttggccggaaaagttggctcaacacctggctacttacggctggttttcaatggctataaaaaagccagttttgtgctggctaaaaaacttgagcaatacacatcaggtgcaattacgaaatctgacttaagaccggatatctatccgaaagattagaaagaggagaaatacacgatggttcgtgcaaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagtgtccgtgctgggaaactggcggggaacttttagagataaccctccgaattgctggcaaatttctgatgaaatttctccgcgaagcccacatgaactacccccgtttaccctcaaaataagccctgtgacacacataacaccccctaatcgtacccgctcacacgctattttcgaggtgtggttcgccttcggaaacgaatgcccccgccccacttggataaaagacgttaacacctgttagtctataacgcgggttgaaccgagaaacccctcaaggcagcagacaatagccgcaaggggttttgcggagcacgtcccctgtgatcgttgcgctgatgtgcgacggagtccgaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataaaatctatcaccgcaagggataaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcatgtactaaggaggttgaaagaggagaaatacacgatgcaatatgccattgcagggtggcctgttgctggctgcccttccgaatctttacttgaacgaatcacccgtaaattacgtgacggatggaaacgccttatcgacatacttaatcagccaggagtcccaaagaatggatcaaacacttatggctatccagactaatactagagattaaagaggagaaatactagatgattatttccgcagccagcgattatcgcgccgcagcgcaacgcattctgccgccgttcctgttccactatatggatggtggtgcatattctgaatacacgctgcgccgcaacgtggaagatttgtcagaagtggcgctgcgccagcgtattctgaaaaacatgtccgacttaagcctggaaacgacgctgtttaatgagaaattgtcgatgccggtggcactggctccggtgggtttgtgtggcatgtatgcgcgtcgtggcgaagttcaggcagccaaagcggcggacgcgcatggtattccgtttactctctcgacggtttccgtttgcccgattgaagaagtcgcgccagccatcaagcgcccaatgtggttccagctttatgtactgcgcgatcgcggctttatgcgtaacgcgctggagcgagcaaaagcagcgggttgttcgacgctggttttcaccgtggatatgccgacaccgggcgcacgctaccgtgatgcgcattcaggtatgagcggcccgaacgcggcaatgcgccgctacttgcaagcggtgacacatccgcaatgggcgtgggatgtgggcctgaacggtcgtccacatgatttaggtaatatctcagcttatctcggcaaaccgaccggactggaagattacatcggctggctggggaataacttcgatccgtccatctcatggaaagaccttgaatggatccgcgatttctgggatggcccgatggtgatcaaagggatcctcgatccggaagatgcgcgcgatgcagtacgttttggtgctgatggaattgtggtttctaaccacggtggccgccagctggacggtgtactctcttccgcccgtgcactgcctgctattgcagatgcggtgaaaggtgatatagccattctggcggatagcggtattcgtaacgggcttgatgtcgtgcgtatgattgcgctcggtgccgacaccgtactgctgggtcgtgctttcttgtatgcgctggcaacagcgggccaggcgggtgtagctaacctgctaaatctgatcgaaaaagagatgaaagtggcgatgacgctgactggcgcgaaatcgatcagcgaaattacgcaagattcgctggtgcaggggctgggtaaagagttgcctgcggcactggctcccatggcgaaagggaatgcggcatagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataaaatagtcaacacgcacggtgttagatatttatcccttgcggtgatagatttaacgtaattaaagaggagaaatactagatgaccatgattacgccaagcttgcatgcgtgcaggtcgactcttgaggatccccgggtaccgagctccaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaa BBa_K2036022_sequence 1 agtgtccgtgctgggaaactggcggggaacttttagagataaccctccgaattgctggcaaatttctgatgaaatttctccgcgaagcccacatgaactacccccgtttaccctcaaaataagccctgtgacacacataacaccccctaatcgtacccgctcacacgctattttcgaggtgtggttcgccttcggaaacgaatgcccccgccccacttggataaaagacgttaacacctgttagtctataacgcgggttgaaccgagaaacccctcaaggcagcagacaatagccgcaaggggttttgcggagcacgtcccctgtgatcgttgcgctgatgtgcgacggagtccgaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataaaatctatcaccgcaagggataaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcatgtactaaggaggttgaaagaggagaaatacacgatgcaatatgccattgcagggtggcctgttgctggctgcccttccgaatctttacttgaacgaatcacccgtaaattacgtgacggatggaaacgccttatcgacatacttaatcagccaggagtcccaaagaatggatcaaacacttatggctatccagactaa BBa_K2036023_sequence 1 attaaagaggagaaatactagatgattatttccgcagccagcgattatcgcgccgcagcgcaacgcattctgccgccgttcctgttccactatatggatggtggtgcatattctgaatacacgctgcgccgcaacgtggaagatttgtcagaagtggcgctgcgccagcgtattctgaaaaacatgtccgacttaagcctggaaacgacgctgtttaatgagaaattgtcgatgccggtggcactggctccggtgggtttgtgtggcatgtatgcgcgtcgtggcgaagttcaggcagccaaagcggcggacgcgcatggtattccgtttactctctcgacggtttccgtttgcccgattgaagaagtcgcgccagccatcaagcgcccaatgtggttccagctttatgtactgcgcgatcgcggctttatgcgtaacgcgctggagcgagcaaaagcagcgggttgttcgacgctggttttcaccgtggatatgccgacaccgggcgcacgctaccgtgatgcgcattcaggtatgagcggcccgaacgcggcaatgcgccgctacttgcaagcggtgacacatccgcaatgggcgtgggatgtgggcctgaacggtcgtccacatgatttaggtaatatctcagcttatctcggcaaaccgaccggactggaagattacatcggctggctggggaataacttcgatccgtccatctcatggaaagaccttgaatggatccgcgatttctgggatggcccgatggtgatcaaagggatcctcgatccggaagatgcgcgcgatgcagtacgttttggtgctgatggaattgtggtttctaaccacggtggccgccagctggacggtgtactctcttccgcccgtgcactgcctgctattgcagatgcggtgaaaggtgatatagccattctggcggatagcggtattcgtaacgggcttgatgtcgtgcgtatgattgcgctcggtgccgacaccgtactgctgggtcgtgctttcttgtatgcgctggcaacagcgggccaggcgggtgtagctaacctgctaaatctgatcgaaaaagagatgaaagtggcgatgacgctgactggcgcgaaatcgatcagcgaaattacgcaagattcgctggtgcaggggctgggtaaagagttgcctgcggcactggctcccatggcgaaagggaatgcggcatagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataaaatagtcaacacgcacggtgttagatatttatcccttgcggtgatagatttaacgtaattaaagaggagaaatactagatgaccatgattacgccaagcttgcatgcgtgcaggtcgactcttgaggatccccgggtaccgagctccaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z