LOCUS BBa_K2036027 3676 bp DNA linear 29-Nov-2024 DEFINITION pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAta g-TT-pRM-RBS-GFP-LVAssrAtag ACCESSION BBa_K2036027 VERSION BBa_K2036027.1 FEATURES Location/Qualifiers promoter 1..52 /label=pRE RBS 53..64 /label=RBS core CDS 75..274 /label=Cro RBS 275..286 /label=RBS core CDS 293..586 /label=CII stem_loop 587..715 /label=terminator misc_feature 716..721 /label=scar promoter 722..806 /label=ptrp2 misc_feature 1..806 /label=BBa_K2036018 RBS 815..826 /label=RBS core misc_feature 827..832 /label=Interval sequence CDS 833..1546 /label=CI stem_loop 1547..1675 /label=stem_loop promoter 1676..1769 /label=pR RBS 1770..1781 /label=RBS core misc_feature 1782..1787 /label=Interval sequence CDS 1788..1952 /label=CIII misc_feature 1953..1960 /label=scar RBS 1961..1972 /label=RBS-core misc_feature 1973..1978 /label=Interval sequence CDS 1979..2854 /label=RFP-LAAssrAtag misc_feature 2855..2862 /label=scar promoter 2863..2899 /label=pRM RBS 2900..2911 /label=RBS core misc_feature 2912..2917 /label=Interval sequence CDS 2918..3676 /label=GFP-LVAssrAtag misc_feature 815..3676 /label=BBa_K2036020 ORIGIN 1 agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttag atattaaaga 61 ggagaaatac acgatgaaag cgtattggga ctctttaacc aaagaacagc agggcgagtt 121 ggccggaaaa gttggctcaa cacctggcta cttacggctg gttttcaatg gctataaaaa 181 agccagtttt gtgctggcta aaaaacttga gcaatacaca tcaggtgcaa ttacgaaatc 241 tgacttaaga ccggatatct atccgaaaga ttagaaagag gagaaataca cgatggttcg 301 tgcaaacaaa cgcaacgagg ctctacgaat cgagagtgcg ttgcttaaca aaatcgcaat 361 gcttggaact gagaagacag cggaagctgt gggcgttgat aagtcgcaga tcagcaggtg 421 gaagagggac tggattccaa agttctcaat gctgcttgct gttcttgaat ggggggtcgt 481 tgacgacgac atggctcgat tggcgcgaca agttgctgcg attctcacca ataaaaaacg 541 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaccag gcatcaaata 601 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 661 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 721 gtggcaaata ttctgaaatg agctgttgac aattaatcat cgaactggtt aactcgtacg 781 caagttcacg taaaaagggt atcgactact agagaaagag gagaaatact agatgagcac 841 aaaaaagaaa ccattaacac aagagcagct tgaggacgca cgtcgcctta aagcaattta 901 tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca agatggggat 961 ggggcagtca ggcgttggtg ctttatttaa tggcatcaat gcattaaatg cttataacgc 1021 cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa tttagccctt caatcgccag 1081 agaaatctac gagatgtatg aagcggttag tatgcagccg tcacttagaa gtgagtatga 1141 gtaccctgtt ttttctcatg ttcaggcagg gatgttctca cctgagctta gaacctttac 1201 caaaggtgat gcggagagat gggtaagcac aaccaaaaaa gccagtgatt ctgcattctg 1261 gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc tccaagccaa gctttcctga 1321 cggaatgtta attctcgttg accctgagca ggctgttgag ccaggtgatt tctgcatagc 1381 cagacttggg ggtgatgagt ttaccttcaa gaaactgatc agggatagcg gtcaggtgtt 1441 tttacaacca ctaaacccac agtacccaat gatcccatgc aatgagagtt gttccgttgt 1501 ggggaaagtt atcgctagtc agtggcctga agagacgttt ggctgaccag gcatcaaata 1561 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 1621 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttataaaatc 1681 tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac ctctggcggt 1741 gataatggtt gcatgtacta aggaggttga aagaggagaa atacacgatg caatatgcca 1801 ttgcagggtg gcctgttgct ggctgccctt ccgaatcttt acttgaacga atcacccgta 1861 aattacgtga cggatggaaa cgccttatcg acatacttaa tcagccagga gtcccaaaga 1921 atggatcaaa cacttatggc tatccagact aatactagag aaagaggaga aatactagat 1981 ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc 2041 cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac 2101 ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct 2161 gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga 2221 ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga 2281 cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa 2341 agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat 2401 gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat 2461 caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta 2521 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat 2581 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca 2641 ctccaccggt gctgctgcaa acgacgaaaa ctacgctctg gctgcttaat aacgctgata 2701 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 2761 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 2821 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agttagatat ttatcccttg 2881 cggtgataga tttaacgtaa aagaggagaa atactagatg cgtaaaggag aagaactttt 2941 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc 3001 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg 3061 cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt 3121 tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat 3181 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac 3241 acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat 3301 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca 3361 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag 3421 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat 3481 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc 3541 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg 3601 gattacacat ggcatggatg aactatacaa agctgcaaac gacgaaaact acgctttagt 3661 agcttaataa taataa //