LOCUS BBa_K2036030 6051 bp DNA linear 23-Dec-2024 DEFINITION PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-prd29 A-Kozak-GFP-LVAssrAtag ACCESSION BBa_K2036030 VERSION BBa_K2036030.1 FEATURES Location/Qualifiers CDS 1..1200 /label=PP2CA misc_feature 207..207 /label=s_mutation misc_feature 264..264 /label=s_mutation misc_feature 877..877 /label=s_mutation misc_feature 1201..1549 /label=TTADH1 promoter 1550..1793 /label=pCyc misc_feature 1..1793 /label=BBa_K2036031 RBS 1802..1813 /label=Kozak CDS 1814..2902 /label=SnRK2.2 misc_feature 2903..3251 /label=BBa_K801012 misc_feature 3252..3495 /label=BBa_I766555 misc_feature 1802..3495 /label=BBa_K2036025 RBS 3504..3515 /label=Kozak CDS 3516..4766 /label=ABF2 misc_feature 4767..5115 /label=TTADH1 promoter 5116..5295 /label=prd29A CDS 5296..6051 /label=GFP-LVAssrA misc_feature 3504..6051 /label=BBa_K2036029 ORIGIN 1 atggctggga tttgttgcgg tgttgttgga gagactgaac cggcggcacc ggttgattca 61 acttctcgag cttctcttag acggaggtta gatctacttc cgtcgatcaa aattgttgct 121 gactccgccg ttgctcctcc gcttgaaaac tgccgtaaac ggcagaagcg tgagacagta 181 gtgctctcga cgttaccggg aaatcttgat ctggattcga atgtgaggag tgagaataag 241 aaggctagat cagcggttac gaactcaaat tctgttacgg aagcagagag tttcttttcc 301 gatgtaccta agatcggtac gacgtcggtt tgtggtagaa gacgagacat ggaagacgct 361 gtctcgattc atccttcgtt tcttcaacgg aactctgaga atcatcattt ctacggtgtc 421 tttgacggcc atggctgctc tcatgttgcg gagaagtgca gagaacggtt gcatgatata 481 gtgaagaagg aagttgaagt tatggcttcc gacgagtgga cagagacgat ggttaagagt 541 tttcaaaaaa tggataaaga agttagccaa cgagagtgta acttagttgt taatggtgct 601 actcggagta tgaaaaattc ttgtagatgt gaattgcagt ctcctcagtg tgacgccgtc 661 ggatccaccg ctgttgtatc ggttgtcacg ccggagaaga tcattgtttc taattgcggt 721 gattcacggg ctgtgctttg tcgtaacggt gtagccattc ctctctccgt agatcacaag 781 ccggatcgac ccgatgaatt gattcggatc caacaagctg gtgggcgagt tatatattgg 841 gatggagcta gggttcttgg agttcttgcc atgtctcgag caattggtga taattacttg 901 aaaccgtatg tgattccgga tccggaggta acagtgacgg atcgaactga tgaggatgag 961 tgtttgatct tggcgagtga tggactatgg gatgttgtac caaacgagac ggcgtgtggc 1021 gtggctcgca tgtgccttcg aggtgctggt gcgggtgacg actcagatgc ggcgcacaat 1081 gcgtgctccg atgcggcgtt gctcttaaca aagttggctc tagcaagaca gagctccgat 1141 aacgtgagcg tcgttgtggt tgacttgagg aagaggagga ataatcaagc gtcgtcttaa 1201 agctttggac ttcttcgcca gaggtttggt caagtctcca atcaaggttg tcggcttgtc 1261 taccttgcca gaaatttacg aaaagatgga aaagggtcaa atcgttggta gatacgttgt 1321 tgacacttct aaataagcga atttcttatg atttatgatt tttattatta aataagttat 1381 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt 1441 attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc 1501 tcttattgac cacacctcta ccggccggtc gaaattcccc taccctatgc agatccgcca 1561 ggcgtgtata tatagcgtgg atggccaggc aactttagtg ctgacacata caggcatata 1621 tatatgtgtg cgacgacaca tgatcatatg gcatgcatgt gctctgtatg tatataaaac 1681 tcttgttttc ttcttttctc taaatattct ttccttatac attaggacct ttgcagcata 1741 aattactata cttctataga cacacaaaca caaatacaca cactaaatta atatactaga 1801 gcccgccgcc accatggatc cggcgactaa ttcaccgatt atgccgattg atttaccgat 1861 tatgcacgac agtgatcgtt acgacttcgt taaagatatt ggctctggta atttcggcgt 1921 tgctcgtctc atgaccgata gagtcaccaa ggagcttgtt gctgttaaat acatcgagag 1981 aggagaaaag attgatgaaa atgttcagag ggagattatc aatcatagat cattgagaca 2041 tcctaatatt gttaggttta aagaggtgat tttgacgcct tcccatttgg ctattgttat 2101 ggaatatgct gctggtggag aactttatga gcggatttgt aatgccggac ggtttagtga 2161 agatgaggct cggttcttct ttcagcagct tatatctgga gttagctatt gtcatgcaat 2221 gcaaatatgc catcgggatc tgaagctgga aaatacattg ttagatggaa gtccggcacc 2281 tcgtttgaaa atatgtgatt ttggttattc caagtcttct gttcttcatt cccaaccaaa 2341 gtcaactgtt ggtactcctg catacattgc accagagatt cttcttcgac aggaatatga 2401 tggcaagctt gcagatgtat ggtcttgcgg tgtaacatta tatgtaatgt tggttggagc 2461 ttatccattc gaggatccac aggagccacg agattatcga aagacaatac aaagaatcct 2521 tagtgtcaca tactcgatcc cagaggactt acacctctca ccagaatgtc gccatctaat 2581 atcgaggatc ttcgtggctg atccggcaac aagaatcact attccggaga tcacatccga 2641 taaatggttc ttgaagaatc taccaggtga tttgatggat gagaaccgaa tgggaagtca 2701 gtttcaagag cctgagcagc caatgcagag ccttgacacg attatgcaga taatatcgga 2761 ggctacgatt ccgactgttc gtaatcgttg cctcgatgat ttcatggcgg ataatcttga 2821 tcttgacgat gacatggatg actttgattc cgaatctgag attgatgttg acagtagtgg 2881 agagatagtt tatgctctct gaagctttgg acttcttcgc cagaggtttg gtcaagtctc 2941 caatcaaggt tgtcggcttg tctaccttgc cagaaattta cgaaaagatg gaaaagggtc 3001 aaatcgttgg tagatacgtt gttgacactt ctaaataagc gaatttctta tgatttatga 3061 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt 3121 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt 3181 ctcaggtata gcatgaggtc gctcttattg accacacctc taccggccgg tcgaaattcc 3241 cctaccctat gcagatccgc caggcgtgta tatatagcgt ggatggccag gcaactttag 3301 tgctgacaca tacaggcata tatatatgtg tgcgacgaca catgatcata tggcatgcat 3361 gtgctctgta tgtatataaa actcttgttt tcttcttttc tctaaatatt ctttccttat 3421 acattaggac ctttgcagca taaattacta tacttctata gacacacaaa cacaaataca 3481 cacactaaat taatatacta gagcccgccg ccaccatgga tggtagtatg aatttgggga 3541 atgagccacc aggagatggt ggtggaggtg gagggttgac tagacaaggt tcgatatact 3601 cgttgacgtt tgatgagttt cagagcagtg tagggaaaga ttttgggtca atgaacatgg 3661 atgagttgtt aaagaatata tggagtgctg aagaaacaca agccatggct agtggtgtgg 3721 ttccagttct tggtggaggt caagagggtt tgcagctgca gaggcaaggc tcgttgactc 3781 tgcctcgaac gcttagtcag aagacggttg atcaagtttg gaaagatcta tccaaagttg 3841 gaagtagtgg agtaggggga agtaacttgt ctcaggtggc tcaggctcag agtcagagtc 3901 agagtcagag gcagcaaaca ttaggtgaag taactttgga ggagtttttg gttcgtgctg 3961 gtgttgtgag agaggaagct caggttgctg caagagctca gattgctgag aacaataaag 4021 gcggttactt tggtaatgat gccaacacag gtttctctgt cgagtttcag cagccttctc 4081 cacgagttgt tgccgctggt gtaatgggaa atcttggtgc agagactgca aattctttgc 4141 aggttcaagg ttctagtttg cctctgaatg tgaatggagc tagaacaaca taccagcaat 4201 cgcaacagca acagccaatc atgcctaagc agcctggttt tggttatgga acacaaatgg 4261 gtcagcttaa tagtcctggg ataagaggtg gtggtcttgt gggacttgga gatcagtctt 4321 taacgaacaa tgtgggcttt gtccaaggtg cttctgctgc aattcctgga gctttaggcg 4381 ttggtgctgt gtcgcctgtt acgccattgt catcagaagg gatagggaag agtaatggtg 4441 attcttcatc actctctccg tctccttaca tgtttaatgg tggtgtgaga ggtagaaaga 4501 gtggcactgt ggagaaagtt gtagagagaa ggcaaaggag aatgataaag aaccgagaat 4561 cagctgcaag gtcccgggcc aggaaacagg cttacaccgt ggagcttgaa gctgaagttg 4621 caaagttaaa ggaagagaat gacgagttac aacgaaagca ggcaaggatc atggaaatgc 4681 aaaagaatca ggagacggag atgaggaatc ttctgcaagg aggtccaaag aaaaagctga 4741 ggaggacaga gtcgggacct tggtgaagct ttggacttct tcgccagagg tttggtcaag 4801 tctccaatca aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag 4861 ggtcaaatcg ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt 4921 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac 4981 tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg 5041 ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg ccggtcgaaa 5101 ttcccctacc ctatggatat actaccgaca tgagttccaa aaagcaaaaa aaaagatcaa 5161 gccgacacag acacgcgtag agagcaaaat gactttgacg tcacaccacg aaaacagacg 5221 cttcatacgt gtccctttat ctctctcagt ctctctataa acttagtgag accctcctct 5281 gttttactca caaatatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt 5341 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt 5401 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt 5461 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca 5521 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa 5581 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa 5641 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac 5701 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac 5761 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc 5821 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta 5881 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga 5941 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa 6001 ctatacaaag ctgcaaacga cgaaaactac gctttagtag cttaataata a //