BBa_K2037000 1 BBa_K2037000 SP6 Promoter 2016-10-11T11:00:00Z 2016-10-12T03:41:10Z SP6 bacteriophage. SP6 phage promoter that is recognised by SP6 RNA polymerase. This part can be used to assemble a device which can be expressed in vitro, by making use of the bacteriophage SP6 RNA Polymerase. false false _2504_ 26774 26774 9 false None false Ricu Claassens BBa_K2037007 1 BBa_K2037007 Laccase for in vitro expression 2016-10-11T11:00:00Z 2016-10-12T05:09:07Z Made from 3 subparts SP6 promoter attached to Laccase 'EucgrG03098' protein sequence with a His (6x) tag after the start codon. T7 terminator at the end. false false _2504_ 26774 26774 9 false None false Ricu Claassens component2495327 1 BBa_K731721 component2495324 1 BBa_K2037000 component2495325 1 BBa_K2037003 annotation2495324 1 BBa_K2037000 range2495324 1 1 18 annotation2495327 1 BBa_K731721 range2495327 1 1767 1814 annotation2495325 1 BBa_K2037003 range2495325 1 25 1758 BBa_K2037003 1 BBa_K2037003 Laccase from Eucalyptus grandis 2016-10-11T11:00:00Z 2016-10-12T04:39:58Z Eucalyptus grandis Laccase 'EucgrG03098' protein from Eucalyptus grandis with His (x6) tag after start codon. false false _2504_ 26774 26774 9 false His tag after start codon. Optimised for E. coli. false Ricu Claassens BBa_K731721 1 BBa_K731721 T7 terminator 2012-08-14T11:00:00Z 2015-05-08T01:13:06Z We amplified it directly from pET21b. Released HQ 2013 This is a short terminator by T7phage that we tested with our new teminators' characterization system. false false _977_ 0 12063 9 In stock true This is a standard sequence, very used in many constructs. As i don't have any design considerations i can say that generally to make screening gels of such short segments is preferable to use TBE1X instead of TAE1X. false Giacomo Giacomelli, Anna Depetris annotation2180632 1 T7 terminator range2180632 1 1 48 BBa_K2037003_sequence 1 atgcatcatcaccatcaccatgccgccgtaggcaagacatcgtttctgctgggagcgctgttattgttctctgttgcagttacgcttgcggatgcaaaggtctactaccacgatttcgtcgtacaggccactaaagtgaaacgtctttgtacgacacataataccatcactgtaaacggtcaattccctggtccaacgctggaagtcaatgatggagacactttagttgttaatgttgttaacaaagcccgttacaacgtgacaatccattggcatggcgttcgtcaggtacgtagcgggtgggctgatggtccagagttcgtaactcaatgccccattcgtccgggaggctcatacacgtatcgcttcacgatccagggacaggtaggcactttgtggtggcatgcccacagctcatggttacgcgccacagtatatggagcgctggtgattcgccctaaggagggaacgtcctacccatttcctaaaccaaaacgtgagaccccaatcttgctgggcgaatggtgggatgctaatccgatcgacgtggtacgtgaagcgacgcgtacaggcggcgcgcccaatgtaagcgacgcctatacgatcaatggacagccgggcgatttatataattgttcgagtaaagataccgtgatcgtgccaatcgattctggagagacacatttattacgcgttattaacgctgctctgaatcaggagctgtttttcaccgttgccaatcaccgtttcactgtagtaggtgctgacgcgtcttaccttaaacccttcaccacgtcggtgattatgctgggtccaggacagaccactgacgtgttaatttctggtgatcaaccaccggcccgctattacatggccgcagagccgtatcagtctgctcagggtgcaccattcgataacaccacaaccaccgcgatcttagagtataaatcagcaccttgccccgcgaaggggattagctccaaacccgtgatgccgacgttacccgcgttcaatgacactgctactgtaacggctttcatccaaagttttcgttccccgaacaaagtggatgtcccaacagatattgacgaaaaccttttcatcacagtaggacttgggctttttaactgccctaaaaactttggctcatctcgctgccaagggcctaatggcacacgtttcacggcttctatgaacaatgtttccttcgtattgcccagcaacgtgtctattttgcaagcctacaaacaaggcgtccccggcgtcttcaccacagacttccccgccaatccacctgttcagttcgattatactggcaatgtgagtcgtagtttgtggcagccagtgcctggcactaaagtgtacaagctgaagtacgggagccgcgtacagattgtattacaaggaacgaacattcaaaccgctgaaaatcatcctattcatattcacggctacgatttttatattctggccaccggatttggtaacttcaaccctcaaaaggacacagctaaattcaatctggttgatccccccatgcgtaacacagttggggtatccgtcaacggttgggcggtaatccgtttcgtcgctgacaatccaggtgcttggcttatgcactgccatttggacgttcatatcacctggggattggcagttgtttttttggtggaaaatggtgttggggaattgcaatcgctgcaaccgccaccagccgatctgcctccatgttaa BBa_K2037000_sequence 1 atttaggtgacactatag BBa_K2037007_sequence 1 atttaggtgacactatagtactagatgcatcatcaccatcaccatgccgccgtaggcaagacatcgtttctgctgggagcgctgttattgttctctgttgcagttacgcttgcggatgcaaaggtctactaccacgatttcgtcgtacaggccactaaagtgaaacgtctttgtacgacacataataccatcactgtaaacggtcaattccctggtccaacgctggaagtcaatgatggagacactttagttgttaatgttgttaacaaagcccgttacaacgtgacaatccattggcatggcgttcgtcaggtacgtagcgggtgggctgatggtccagagttcgtaactcaatgccccattcgtccgggaggctcatacacgtatcgcttcacgatccagggacaggtaggcactttgtggtggcatgcccacagctcatggttacgcgccacagtatatggagcgctggtgattcgccctaaggagggaacgtcctacccatttcctaaaccaaaacgtgagaccccaatcttgctgggcgaatggtgggatgctaatccgatcgacgtggtacgtgaagcgacgcgtacaggcggcgcgcccaatgtaagcgacgcctatacgatcaatggacagccgggcgatttatataattgttcgagtaaagataccgtgatcgtgccaatcgattctggagagacacatttattacgcgttattaacgctgctctgaatcaggagctgtttttcaccgttgccaatcaccgtttcactgtagtaggtgctgacgcgtcttaccttaaacccttcaccacgtcggtgattatgctgggtccaggacagaccactgacgtgttaatttctggtgatcaaccaccggcccgctattacatggccgcagagccgtatcagtctgctcagggtgcaccattcgataacaccacaaccaccgcgatcttagagtataaatcagcaccttgccccgcgaaggggattagctccaaacccgtgatgccgacgttacccgcgttcaatgacactgctactgtaacggctttcatccaaagttttcgttccccgaacaaagtggatgtcccaacagatattgacgaaaaccttttcatcacagtaggacttgggctttttaactgccctaaaaactttggctcatctcgctgccaagggcctaatggcacacgtttcacggcttctatgaacaatgtttccttcgtattgcccagcaacgtgtctattttgcaagcctacaaacaaggcgtccccggcgtcttcaccacagacttccccgccaatccacctgttcagttcgattatactggcaatgtgagtcgtagtttgtggcagccagtgcctggcactaaagtgtacaagctgaagtacgggagccgcgtacagattgtattacaaggaacgaacattcaaaccgctgaaaatcatcctattcatattcacggctacgatttttatattctggccaccggatttggtaacttcaaccctcaaaaggacacagctaaattcaatctggttgatccccccatgcgtaacacagttggggtatccgtcaacggttgggcggtaatccgtttcgtcgctgacaatccaggtgcttggcttatgcactgccatttggacgttcatatcacctggggattggcagttgtttttttggtggaaaatggtgttggggaattgcaatcgctgcaaccgccaccagccgatctgcctccatgttaatactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_K731721_sequence 1 ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z