LOCUS       BBa_K2037009            1669 bp    DNA     linear       26-Dec-2024
DEFINITION  Laccase for in vitro expression
ACCESSION   BBa_K2037009
VERSION     BBa_K2037009.1  
FEATURES             Location/Qualifiers
     promoter        1..18
                     /label=BBa_K2037000
     promoter        27..49
                     /label=T7- Promotor BBa_I719005
     RBS             51..56
                     /label=RBS
     misc_feature    60..62
                     /label=ATG
     CDS             60..1582
                     /label=bpul
     misc_feature    860..860
                     /label=A (Original) T (Mutation)
     misc_feature    1583..1607
                     /label=6 x HIS
     misc_feature    1608..1610
                     /label=TAA
     misc_feature    1611..1613
                     /label=TAA
     CDS             27..1613
                     /label=BBa_K863000
     misc_feature    1622..1669
                     /label=T7 terminator
     terminator      1622..1669
                     /label=BBa_K731721
ORIGIN
        1 atttaggtga cactatagta ctagagtaat acgactcact atagggagag aggagaaaaa
       61 tgaacctaga aaaatttgtt gacgagctgc caattccaga agttgcgaag cccgtcaaaa
      121 agaacccaaa acaaacgtat tatgaaatcg ctatggagga ggtatttcta aaagttcata
      181 gagatctgcc cccaaccaag ctatggacct ataatggcag tttgcctggt ccaaccattc
      241 atgcgaatcg aaatgaaaaa gtcaaagtga aatggatgaa caaattgcca cttaagcatt
      301 ttctaccggt cgatcacacc attcacgaag gccatcatga tgaaccagaa gttaaaaccg
      361 tcgttcattt acatggtggc gtcacaccag caagcagtga tggctatcca gaggcttggt
      421 tttcacgaga ctttgaagca accggcccct tctttgaacg ggaggtgtac gaatacccaa
      481 atcatcagca agcctgcaca ttgtggtatc acgatcatgc gatggcattg acacgattaa
      541 atgtgtatgc cggcttagct ggattttatt tgatctcaga tgcgtttgaa aagtcgctag
      601 aattaccgaa gggtgagtat gatattccgc taatgatcat ggaccgtacg tttcaggagg
      661 atggcgcact gttttatcca agcaggccaa acaacacacc agaagacagt gacataccag
      721 atccgtctat cgtgcctttc ttttgcggag aaaccatttt ggtcaatgga aaagtatggc
      781 cgtatttaga agtagagccg cgaaaatatc gttttcgtat tttaaatgct tccaatacaa
      841 gaacttacga gctgcatctt gacaacgatg cgacgatttt gcaaattgga tctgatggcg
      901 gctttttacc aagacctgtt caccatcaat cctttagcat tgctcctgct gaacgatttg
      961 atgtcatcat cgatttttca gcttacgaaa acaaaacgat cacccttaaa aataaagccg
     1021 gctgcggaca ggaagtaaat cctgaaacag atgcaaacat catgcaattt aaagtcactc
     1081 gaccgctaaa agggagagca cctaaaacat tacggcctat tttcaaaccg cttccaccac
     1141 ttcggccttg tcgagctgat aaagagcgta cgctcactct taccggtaca caggataaat
     1201 acggccgtcc tattttattg ctagataacc aattttggaa tgaccctgtc acggaaaatc
     1261 ctcgtcttgg cagtgtggag gtttggtcta tcgtcaatcc aacaaggggc acacatccta
     1321 ttcatttaca ccttgttcaa ttcagagtga tagacagaag accatttgat actgaggtct
     1381 atcaatcgac aggggacatt gtgtatacag gaccaaacga agcacctccc ttacatgaac
     1441 aaggctacaa ggacaccatt caagcgcatg ccggtgaagt cattcggatc atcgctcgct
     1501 ttgttccata cagcggcagg tatgtgtggc attgtcatat attagagcac gaggattatg
     1561 acatgatgcg gccgatggat atcatccagc atcaccatca ccatcactaa taatactaga
     1621 gctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttg
//