BBa_K2037000 1 BBa_K2037000 SP6 Promoter 2016-10-11T11:00:00Z 2016-10-12T03:41:10Z SP6 bacteriophage. SP6 phage promoter that is recognised by SP6 RNA polymerase. This part can be used to assemble a device which can be expressed in vitro, by making use of the bacteriophage SP6 RNA Polymerase. false false _2504_ 26774 26774 9 false None false Ricu Claassens BBa_K863000 1 BBa_K863000 bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag 2012-09-17T11:00:00Z 2015-05-08T01:13:36Z Bacillus pumilus DSM 27 (ATCC7061) Released HQ 2013 bpul (Laccase from Bacillus pumilus) with T7, RBS and HIS tag false false _1123_ 0 12844 9 In stock true test false Isabel Huber annotation2184912 1 A (Original) T (Mutation) range2184912 1 834 834 annotation2184910 1 ATG range2184910 1 34 36 annotation2184908 1 T7- Promotor BBa_I719005 range2184908 1 1 23 annotation2184913 1 6 x HIS range2184913 1 1557 1581 annotation2184911 1 bpul range2184911 1 34 1556 annotation2184909 1 RBS range2184909 1 25 30 annotation2184915 1 TAA range2184915 1 1585 1587 annotation2184914 1 TAA range2184914 1 1582 1584 BBa_K2037009 1 BBa_K2037009 Laccase for in vitro expression 2016-10-11T11:00:00Z 2016-10-12T05:15:34Z Made from 3 subparts SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end. false false _2504_ 26774 26774 9 false None. false Ricu Claassens component2495332 1 BBa_K2037000 component2495341 1 BBa_K863000 component2495343 1 BBa_K731721 annotation2495343 1 BBa_K731721 range2495343 1 1622 1669 annotation2495332 1 BBa_K2037000 range2495332 1 1 18 annotation2495341 1 BBa_K863000 range2495341 1 27 1613 BBa_K731721 1 BBa_K731721 T7 terminator 2012-08-14T11:00:00Z 2015-05-08T01:13:06Z We amplified it directly from pET21b. Released HQ 2013 This is a short terminator by T7phage that we tested with our new teminators' characterization system. false false _977_ 0 12063 9 In stock true This is a standard sequence, very used in many constructs. As i don't have any design considerations i can say that generally to make screening gels of such short segments is preferable to use TBE1X instead of TAE1X. false Giacomo Giacomelli, Anna Depetris annotation2180632 1 T7 terminator range2180632 1 1 48 BBa_K863000_sequence 1 taatacgactcactatagggagagaggagaaaaatgaacctagaaaaatttgttgacgagctgccaattccagaagttgcgaagcccgtcaaaaagaacccaaaacaaacgtattatgaaatcgctatggaggaggtatttctaaaagttcatagagatctgcccccaaccaagctatggacctataatggcagtttgcctggtccaaccattcatgcgaatcgaaatgaaaaagtcaaagtgaaatggatgaacaaattgccacttaagcattttctaccggtcgatcacaccattcacgaaggccatcatgatgaaccagaagttaaaaccgtcgttcatttacatggtggcgtcacaccagcaagcagtgatggctatccagaggcttggttttcacgagactttgaagcaaccggccccttctttgaacgggaggtgtacgaatacccaaatcatcagcaagcctgcacattgtggtatcacgatcatgcgatggcattgacacgattaaatgtgtatgccggcttagctggattttatttgatctcagatgcgtttgaaaagtcgctagaattaccgaagggtgagtatgatattccgctaatgatcatggaccgtacgtttcaggaggatggcgcactgttttatccaagcaggccaaacaacacaccagaagacagtgacataccagatccgtctatcgtgcctttcttttgcggagaaaccattttggtcaatggaaaagtatggccgtatttagaagtagagccgcgaaaatatcgttttcgtattttaaatgcttccaatacaagaacttacgagctgcatcttgacaacgatgcgacgattttgcaaattggatctgatggcggctttttaccaagacctgttcaccatcaatcctttagcattgctcctgctgaacgatttgatgtcatcatcgatttttcagcttacgaaaacaaaacgatcacccttaaaaataaagccggctgcggacaggaagtaaatcctgaaacagatgcaaacatcatgcaatttaaagtcactcgaccgctaaaagggagagcacctaaaacattacggcctattttcaaaccgcttccaccacttcggccttgtcgagctgataaagagcgtacgctcactcttaccggtacacaggataaatacggccgtcctattttattgctagataaccaattttggaatgaccctgtcacggaaaatcctcgtcttggcagtgtggaggtttggtctatcgtcaatccaacaaggggcacacatcctattcatttacaccttgttcaattcagagtgatagacagaagaccatttgatactgaggtctatcaatcgacaggggacattgtgtatacaggaccaaacgaagcacctcccttacatgaacaaggctacaaggacaccattcaagcgcatgccggtgaagtcattcggatcatcgctcgctttgttccatacagcggcaggtatgtgtggcattgtcatatattagagcacgaggattatgacatgatgcggccgatggatatcatccagcatcaccatcaccatcactaataa BBa_K2037000_sequence 1 atttaggtgacactatag BBa_K2037009_sequence 1 atttaggtgacactatagtactagagtaatacgactcactatagggagagaggagaaaaatgaacctagaaaaatttgttgacgagctgccaattccagaagttgcgaagcccgtcaaaaagaacccaaaacaaacgtattatgaaatcgctatggaggaggtatttctaaaagttcatagagatctgcccccaaccaagctatggacctataatggcagtttgcctggtccaaccattcatgcgaatcgaaatgaaaaagtcaaagtgaaatggatgaacaaattgccacttaagcattttctaccggtcgatcacaccattcacgaaggccatcatgatgaaccagaagttaaaaccgtcgttcatttacatggtggcgtcacaccagcaagcagtgatggctatccagaggcttggttttcacgagactttgaagcaaccggccccttctttgaacgggaggtgtacgaatacccaaatcatcagcaagcctgcacattgtggtatcacgatcatgcgatggcattgacacgattaaatgtgtatgccggcttagctggattttatttgatctcagatgcgtttgaaaagtcgctagaattaccgaagggtgagtatgatattccgctaatgatcatggaccgtacgtttcaggaggatggcgcactgttttatccaagcaggccaaacaacacaccagaagacagtgacataccagatccgtctatcgtgcctttcttttgcggagaaaccattttggtcaatggaaaagtatggccgtatttagaagtagagccgcgaaaatatcgttttcgtattttaaatgcttccaatacaagaacttacgagctgcatcttgacaacgatgcgacgattttgcaaattggatctgatggcggctttttaccaagacctgttcaccatcaatcctttagcattgctcctgctgaacgatttgatgtcatcatcgatttttcagcttacgaaaacaaaacgatcacccttaaaaataaagccggctgcggacaggaagtaaatcctgaaacagatgcaaacatcatgcaatttaaagtcactcgaccgctaaaagggagagcacctaaaacattacggcctattttcaaaccgcttccaccacttcggccttgtcgagctgataaagagcgtacgctcactcttaccggtacacaggataaatacggccgtcctattttattgctagataaccaattttggaatgaccctgtcacggaaaatcctcgtcttggcagtgtggaggtttggtctatcgtcaatccaacaaggggcacacatcctattcatttacaccttgttcaattcagagtgatagacagaagaccatttgatactgaggtctatcaatcgacaggggacattgtgtatacaggaccaaacgaagcacctcccttacatgaacaaggctacaaggacaccattcaagcgcatgccggtgaagtcattcggatcatcgctcgctttgttccatacagcggcaggtatgtgtggcattgtcatatattagagcacgaggattatgacatgatgcggccgatggatatcatccagcatcaccatcaccatcactaataatactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_K731721_sequence 1 ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z