LOCUS       BBa_K2037012            1686 bp    DNA     linear       29-Nov-2024
DEFINITION  Laccase For in vitro expression
ACCESSION   BBa_K2037012
VERSION     BBa_K2037012.1  
FEATURES             Location/Qualifiers
     promoter        1..18
                     /label=BBa_K2037000
     promoter        27..49
                     /label=T7 promoter
     misc_feature    60..62
                     /label=ATG
     misc_feature    1629..1631
                     /label=TAA
     misc_feature    1626..1628
                     /label=TAA
     misc_feature    1608..1625
                     /label=6x HIS 
     CDS             60..1607
                     /label=ecol
     RBS             51..56
                     /label=RBS
     CDS             27..1631
                     /label=BBa_K863005
     stem_loop       1653..1672
                     /label=T7 TE
     misc_feature    1679..1679
                     /label=stop
     misc_feature    1676..1676
                     /label=C
     misc_feature    1649..1649
                     /label=C
     polyA_site      1640..1645
                     /label=''
     polyA_site      1673..1686
                     /label=polya
     misc_feature    1640..1686
                     /label=BBa_B0013
     terminator      1640..1686
                     /label=BBa_B0013
ORIGIN
        1 atttaggtga cactatagta ctagagtaat acgactcact atagggagag aggagaaaaa
       61 tgcaacgtcg tgatttctta aaatattccg tcgcgctggg tgtggcttcg gctttgccgc
      121 tgtggaaccg cgcagtattt gcggcagaac gcccaacgtt accgatccct gatttgctca
      181 cgaccgatgc ccgtaatcgc attcagttaa ctattggcgc aggccagtcc acctttggcg
      241 ggaaaactgc aactacctgg ggctataacg gcaatctgct gggaccggcg gtgaaattac
      301 agcgcggcaa agcggtaacg gttgatatct acaaccaact gacggaagag acaacgttgc
      361 actggcacgg gctggaagta ccgggtgaag tcgacggcgg cccgcaggga attattccgc
      421 caggtggcaa gcgctcggtg acgttgaacg ttgatcaacc tgccgctacc tgctggttcc
      481 atccgcatca gcacggcaaa accgggcgac aggtggcgat ggggctggct gggctggtgg
      541 tgattgaaga tgacgagatc ctgaaattaa tgctgccaaa acagtggggt atcgatgatg
      601 ttccggtgat cgttcaggat aagaaattta gcgccgacgg gcagattgat tatcaactgg
      661 atgtgatgac cgccgccgtg ggctggtttg gcgatacgtt gctgaccaac ggtgcaatct
      721 acccgcaaca cgctgccccg cgtggttggc tgcgcctgcg tttgctcaat ggctgtaatg
      781 cccgttcgct caatttcgcc accagcgaca atcgcccgct gtatgtgatt gccagcgacg
      841 gtggtctgct acctgaacca gtgaaggtga gcgaactgcc ggtgctgatg ggcgagcgtt
      901 ttgaagtgct ggtggaggtt aacgataaca aaccctttga cctggtgacg ctgccggtca
      961 gccagatggg gatggcgatt gcgccgtttg ataagcctca tccggtaatg cggattcagc
     1021 cgattgctat tagtgcctcc ggtgctttgc cagacacatt aagtagcctg cctgcgttac
     1081 cttcgctgga agggctgacg gtacgcaagc tgcaactctc tatggacccg atgctcgata
     1141 tgatggggat gcagatgcta atggagaaat atggcgatca ggcgatggcc gggatggatc
     1201 acagccagat gatgggccat atggggcacg gcaatatgaa tcatatgaac cacggcggga
     1261 agttcgattt ccaccatgcc aacaaaatca acggtcaggc gtttgatatg aacaagccga
     1321 tgtttgcggc ggcgaaaggg caatacgaac gttgggttat ctctggcgtg ggcgacatga
     1381 tgctgcatcc gttccatatc cacggcacgc agttccgtat cttgtcagaa aatggcaaac
     1441 cgccagcggc tcatcgcgcg ggctggaaag ataccgttaa ggtagaaggt aatgtcagcg
     1501 aagtgctggt gaagtttaat cacgatgcac cgaaagaaca tgcttatatg gcgcactgcc
     1561 atctgctgga gcatgaagat acggggatga tgttagggtt tacggtacat caccatcacc
     1621 atcactaata atactagaga aaaaatcaaa ctggctcacc ttcgggtggg cctttttgcg
     1681 tttata
//