LOCUS       BBa_K2037014            1524 bp    DNA     linear       22-Nov-2024
DEFINITION  Laccase for in vitro expression
ACCESSION   BBa_K2037014
VERSION     BBa_K2037014.1  
FEATURES             Location/Qualifiers
     promoter        1..18
                     /label=BBa_K2037000
     misc_feature    60..62
                     /label=ATG
     promoter        27..49
                     /label=T7 promoter
     CDS             60..1445
                     /label=tthl
     misc_feature    1446..1463
                     /label=6x HIS
     misc_feature    1464..1466
                     /label=TAA
     misc_feature    1467..1469
                     /label=TAA
     CDS             27..1469
                     /label=BBa_K863010
     stem_loop       1491..1510
                     /label=T7 TE
     misc_feature    1517..1517
                     /label=stop
     misc_feature    1514..1514
                     /label=C
     misc_feature    1487..1487
                     /label=C
     polyA_site      1478..1483
                     /label=''
     polyA_site      1511..1524
                     /label=polya
     misc_feature    1478..1524
                     /label=BBa_B0013
     terminator      1478..1524
                     /label=BBa_B0013
ORIGIN
        1 atttaggtga cactatagta ctagagtaat acgactcact atagggagag aggagaaaaa
       61 tgctggcgcg caggagcttt ctccaagcgg cggcgggcag tctggtcctg ggcctggccc
      121 gggcccaagg cccttccttc cccgagccca aggtggtgcg gagccagggc ggcctcctct
      181 ccctgaagct ttctgccacc cccaccccgc ttgccctggc ggggcaaagg gccaccctcc
      241 tcacctacgg ggggagcttt cccgggccca ccctccgggt ccgccccagg gacacggtgc
      301 gcctcaccct ggaaaaccgc cttcccgagc ccaccaacct ccactggcac ggcctgccca
      361 tctcccctaa ggtggacgac cccttcctgg agatcccccc gggggagagc tggacctacg
      421 agttcaccgt tcccaaggag ctggcaggta ccttctggta ccacccccac ctgcacggcc
      481 gggtagcccc ccagctcttt gccggcctcc tgggagccct cgtggtggaa agctccctgg
      541 acgccatccc cgagctcagg gaggcggagg agcacctcct cgtcctgaag gacctggccc
      601 tccagggcgg gcgcccggcg ccgcacaccc ccatggactg gatgaacggg aaggagggcg
      661 acctggtcct ggtgaacggg gccctgcggc ccaccctggt ggcccagaag gccaccctga
      721 ggcttcgcct cctcaacgcc tccaacgccc gctactaccg cctggccctg caagaccacc
      781 ccctttacct catcgccgcc gatgggggct tcctggaaga gcccctggag gtgtccgagc
      841 tcctcctggc cccaggagag cgggccgagg tcctggtgcg cttgcggaaa gagggccgct
      901 tcctcctcca ggccctgccc tacgaccgcg gggccatggg catgatggac atggggggca
      961 tggcccacgc catgccccaa gggccaagcc ggcccgaaac ccttctttac ctcatcgccc
     1021 ccaagaaccc caagccctta cccctgccca aggccctaag ccccttcccc accctgcccg
     1081 cccccgtggt cacccgccgc ctggtcctca ccgaggacat gatggccgcc cgcttcttca
     1141 tcaacggcca ggtctttgac cacaggcggg tggacctgaa ggggcaggcc cagacggtgg
     1201 aggtctggga ggtggaaaac cagggggaca tggaccaccc cttccacctc cacgtccacc
     1261 ccttccaggt cctctccgtg ggcgggaggc ctttccccta ccgggcctgg aaggatgtgg
     1321 tcaacctgaa ggcgggcgag gtggccaggc ttctggttcc cttgagggaa aagggccgga
     1381 ccgtttttca ctgccacatc gtggagcacg aggaccgggg gatgatggga gtcctcgagg
     1441 tgggtcatca ccatcaccat cactaataat actagagaaa aaatcaaact ggctcacctt
     1501 cgggtgggcc tttttgcgtt tata
//