BBa_B0013 1 BBa_B0013 TE from coliphage T7 (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream.<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1698 1 polya range1698 1 34 47 annotation1696 1 C range1696 1 10 10 annotation7021 1 BBa_B0013 range7021 1 1 47 annotation1695 1 C range1695 1 37 37 annotation1697 1 '' range1697 1 1 6 annotation1692 1 stop range1692 1 40 40 annotation1691 1 T7 TE range1691 1 14 33 BBa_K2037014 1 BBa_K2037014 Laccase for in vitro expression 2016-10-13T11:00:00Z 2016-10-14T03:15:03Z Thermus thermophilus SP6 promoter joined to a tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag false false _2504_ 26994 26994 9 false None false Vaughn Murray Barendsen component2509489 1 BBa_K863010 component2509491 1 BBa_B0013 component2509482 1 BBa_K2037000 annotation2509491 1 BBa_B0013 range2509491 1 1478 1524 annotation2509489 1 BBa_K863010 range2509489 1 27 1469 annotation2509482 1 BBa_K2037000 range2509482 1 1 18 BBa_K863010 1 BBa_K863010 tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag 2012-09-17T11:00:00Z 2015-05-08T01:13:36Z Thermus thermophilus HB27 Released HQ 2013 tthl (Laccase from Thermus thermophilus) with T7, RBS and HIS tag false false _1123_ 0 12844 9 In stock false test false Isabel Huber annotation2184732 1 tthl range2184732 1 34 1419 annotation2184782 1 TAA range2184782 1 1438 1440 annotation2184671 1 ATG range2184671 1 34 36 annotation2184733 1 6x HIS range2184733 1 1420 1437 annotation2184783 1 TAA range2184783 1 1441 1443 annotation2184708 1 T7 promoter range2184708 1 1 23 BBa_K2037000 1 BBa_K2037000 SP6 Promoter 2016-10-11T11:00:00Z 2016-10-12T03:41:10Z SP6 bacteriophage. SP6 phage promoter that is recognised by SP6 RNA polymerase. This part can be used to assemble a device which can be expressed in vitro, by making use of the bacteriophage SP6 RNA Polymerase. false false _2504_ 26774 26774 9 false None false Ricu Claassens BBa_B0013_sequence 1 aaaaaatcaaactggctcaccttcgggtgggcctttttgcgtttata BBa_K863010_sequence 1 taatacgactcactatagggagagaggagaaaaatgctggcgcgcaggagctttctccaagcggcggcgggcagtctggtcctgggcctggcccgggcccaaggcccttccttccccgagcccaaggtggtgcggagccagggcggcctcctctccctgaagctttctgccacccccaccccgcttgccctggcggggcaaagggccaccctcctcacctacggggggagctttcccgggcccaccctccgggtccgccccagggacacggtgcgcctcaccctggaaaaccgccttcccgagcccaccaacctccactggcacggcctgcccatctcccctaaggtggacgaccccttcctggagatccccccgggggagagctggacctacgagttcaccgttcccaaggagctggcaggtaccttctggtaccacccccacctgcacggccgggtagccccccagctctttgccggcctcctgggagccctcgtggtggaaagctccctggacgccatccccgagctcagggaggcggaggagcacctcctcgtcctgaaggacctggccctccagggcgggcgcccggcgccgcacacccccatggactggatgaacgggaaggagggcgacctggtcctggtgaacggggccctgcggcccaccctggtggcccagaaggccaccctgaggcttcgcctcctcaacgcctccaacgcccgctactaccgcctggccctgcaagaccaccccctttacctcatcgccgccgatgggggcttcctggaagagcccctggaggtgtccgagctcctcctggccccaggagagcgggccgaggtcctggtgcgcttgcggaaagagggccgcttcctcctccaggccctgccctacgaccgcggggccatgggcatgatggacatggggggcatggcccacgccatgccccaagggccaagccggcccgaaacccttctttacctcatcgcccccaagaaccccaagcccttacccctgcccaaggccctaagccccttccccaccctgcccgcccccgtggtcacccgccgcctggtcctcaccgaggacatgatggccgcccgcttcttcatcaacggccaggtctttgaccacaggcgggtggacctgaaggggcaggcccagacggtggaggtctgggaggtggaaaaccagggggacatggaccaccccttccacctccacgtccaccccttccaggtcctctccgtgggcgggaggcctttcccctaccgggcctggaaggatgtggtcaacctgaaggcgggcgaggtggccaggcttctggttcccttgagggaaaagggccggaccgtttttcactgccacatcgtggagcacgaggaccgggggatgatgggagtcctcgaggtgggtcatcaccatcaccatcactaataa BBa_K2037014_sequence 1 atttaggtgacactatagtactagagtaatacgactcactatagggagagaggagaaaaatgctggcgcgcaggagctttctccaagcggcggcgggcagtctggtcctgggcctggcccgggcccaaggcccttccttccccgagcccaaggtggtgcggagccagggcggcctcctctccctgaagctttctgccacccccaccccgcttgccctggcggggcaaagggccaccctcctcacctacggggggagctttcccgggcccaccctccgggtccgccccagggacacggtgcgcctcaccctggaaaaccgccttcccgagcccaccaacctccactggcacggcctgcccatctcccctaaggtggacgaccccttcctggagatccccccgggggagagctggacctacgagttcaccgttcccaaggagctggcaggtaccttctggtaccacccccacctgcacggccgggtagccccccagctctttgccggcctcctgggagccctcgtggtggaaagctccctggacgccatccccgagctcagggaggcggaggagcacctcctcgtcctgaaggacctggccctccagggcgggcgcccggcgccgcacacccccatggactggatgaacgggaaggagggcgacctggtcctggtgaacggggccctgcggcccaccctggtggcccagaaggccaccctgaggcttcgcctcctcaacgcctccaacgcccgctactaccgcctggccctgcaagaccaccccctttacctcatcgccgccgatgggggcttcctggaagagcccctggaggtgtccgagctcctcctggccccaggagagcgggccgaggtcctggtgcgcttgcggaaagagggccgcttcctcctccaggccctgccctacgaccgcggggccatgggcatgatggacatggggggcatggcccacgccatgccccaagggccaagccggcccgaaacccttctttacctcatcgcccccaagaaccccaagcccttacccctgcccaaggccctaagccccttccccaccctgcccgcccccgtggtcacccgccgcctggtcctcaccgaggacatgatggccgcccgcttcttcatcaacggccaggtctttgaccacaggcgggtggacctgaaggggcaggcccagacggtggaggtctgggaggtggaaaaccagggggacatggaccaccccttccacctccacgtccaccccttccaggtcctctccgtgggcgggaggcctttcccctaccgggcctggaaggatgtggtcaacctgaaggcgggcgaggtggccaggcttctggttcccttgagggaaaagggccggaccgtttttcactgccacatcgtggagcacgaggaccgggggatgatgggagtcctcgaggtgggtcatcaccatcaccatcactaataatactagagaaaaaatcaaactggctcaccttcgggtgggcctttttgcgtttata BBa_K2037000_sequence 1 atttaggtgacactatag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z