BBa_B0013 1 BBa_B0013 TE from coliphage T7 (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream.<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7021 1 BBa_B0013 range7021 1 1 47 annotation1695 1 C range1695 1 37 37 annotation1696 1 C range1696 1 10 10 annotation1691 1 T7 TE range1691 1 14 33 annotation1692 1 stop range1692 1 40 40 annotation1698 1 polya range1698 1 34 47 annotation1697 1 '' range1697 1 1 6 BBa_K2037000 1 BBa_K2037000 SP6 Promoter 2016-10-11T11:00:00Z 2016-10-12T03:41:10Z SP6 bacteriophage. SP6 phage promoter that is recognised by SP6 RNA polymerase. This part can be used to assemble a device which can be expressed in vitro, by making use of the bacteriophage SP6 RNA Polymerase. false false _2504_ 26774 26774 9 false None false Ricu Claassens BBa_K2037015 1 BBa_K2037015 Laccase for in vitro expression 2016-10-13T11:00:00Z 2016-10-14T03:18:36Z Thermus thermophiles SP6 promoter Joined to tthl laccase from Thermus thermophiles and a T7 terminator false false _2504_ 26994 26994 9 false None false Vaughn Murray Barendsen component2509502 1 BBa_K2037000 component2509508 1 BBa_B0013 component2509506 1 BBa_K863011 annotation2509502 1 BBa_K2037000 range2509502 1 1 18 annotation2509506 1 BBa_K863011 range2509506 1 25 1413 annotation2509508 1 BBa_B0013 range2509508 1 1422 1468 BBa_K863011 1 BBa_K863011 tthl laccase from Thermus thermophilus 2012-09-17T11:00:00Z 2015-05-08T01:13:36Z Thermus thermophilus HB27 Released HQ 2013 tthl (Laccase from Thermus thermophilus) coding sequence false false _1123_ 0 12844 9 In stock false test false Isabel Huber annotation2184888 1 tthl range2184888 1 1 1389 annotation2184889 1 TAG range2184889 1 1387 1389 annotation2184806 1 ATG range2184806 1 1 3 BBa_K863011_sequence 1 atgctggcgcgcaggagctttctccaagcggcggcgggcagtctggtcctgggcctggcccgggcccaaggcccttccttccccgagcccaaggtggtgcggagccagggcggcctcctctccctgaagctttctgccacccccaccccgcttgccctggcggggcaaagggccaccctcctcacctacggggggagctttcccgggcccaccctccgggtccgccccagggacacggtgcgcctcaccctggaaaaccgccttcccgagcccaccaacctccactggcacggcctgcccatctcccctaaggtggacgaccccttcctggagatccccccgggggagagctggacctacgagttcaccgttcccaaggagctggcaggtaccttctggtaccacccccacctgcacggccgggtagccccccagctctttgccggcctcctgggagccctcgtggtggaaagctccctggacgccatccccgagctcagggaggcggaggagcacctcctcgtcctgaaggacctggccctccagggcgggcgcccggcgccgcacacccccatggactggatgaacgggaaggagggcgacctggtcctggtgaacggggccctgcggcccaccctggtggcccagaaggccaccctgaggcttcgcctcctcaacgcctccaacgcccgctactaccgcctggccctgcaagaccaccccctttacctcatcgccgccgatgggggcttcctggaagagcccctggaggtgtccgagctcctcctggccccaggagagcgggccgaggtcctggtgcgcttgcggaaagagggccgcttcctcctccaggccctgccctacgaccgcggggccatgggcatgatggacatggggggcatggcccacgccatgccccaagggccaagccggcccgaaacccttctttacctcatcgcccccaagaaccccaagcccttacccctgcccaaggccctaagccccttccccaccctgcccgcccccgtggtcacccgccgcctggtcctcaccgaggacatgatggccgcccgcttcttcatcaacggccaggtctttgaccacaggcgggtggacctgaaggggcaggcccagacggtggaggtctgggaggtggaaaaccagggggacatggaccaccccttccacctccacgtccaccccttccaggtcctctccgtgggcgggaggcctttcccctaccgggcctggaaggatgtggtcaacctgaaggcgggcgaggtggccaggcttctggttcccttgagggaaaagggccggaccgtttttcactgccacatcgtggagcacgaggaccgggggatgatgggagtcctcgaggtgggttag BBa_B0013_sequence 1 aaaaaatcaaactggctcaccttcgggtgggcctttttgcgtttata BBa_K2037000_sequence 1 atttaggtgacactatag BBa_K2037015_sequence 1 atttaggtgacactatagtactagatgctggcgcgcaggagctttctccaagcggcggcgggcagtctggtcctgggcctggcccgggcccaaggcccttccttccccgagcccaaggtggtgcggagccagggcggcctcctctccctgaagctttctgccacccccaccccgcttgccctggcggggcaaagggccaccctcctcacctacggggggagctttcccgggcccaccctccgggtccgccccagggacacggtgcgcctcaccctggaaaaccgccttcccgagcccaccaacctccactggcacggcctgcccatctcccctaaggtggacgaccccttcctggagatccccccgggggagagctggacctacgagttcaccgttcccaaggagctggcaggtaccttctggtaccacccccacctgcacggccgggtagccccccagctctttgccggcctcctgggagccctcgtggtggaaagctccctggacgccatccccgagctcagggaggcggaggagcacctcctcgtcctgaaggacctggccctccagggcgggcgcccggcgccgcacacccccatggactggatgaacgggaaggagggcgacctggtcctggtgaacggggccctgcggcccaccctggtggcccagaaggccaccctgaggcttcgcctcctcaacgcctccaacgcccgctactaccgcctggccctgcaagaccaccccctttacctcatcgccgccgatgggggcttcctggaagagcccctggaggtgtccgagctcctcctggccccaggagagcgggccgaggtcctggtgcgcttgcggaaagagggccgcttcctcctccaggccctgccctacgaccgcggggccatgggcatgatggacatggggggcatggcccacgccatgccccaagggccaagccggcccgaaacccttctttacctcatcgcccccaagaaccccaagcccttacccctgcccaaggccctaagccccttccccaccctgcccgcccccgtggtcacccgccgcctggtcctcaccgaggacatgatggccgcccgcttcttcatcaacggccaggtctttgaccacaggcgggtggacctgaaggggcaggcccagacggtggaggtctgggaggtggaaaaccagggggacatggaccaccccttccacctccacgtccaccccttccaggtcctctccgtgggcgggaggcctttcccctaccgggcctggaaggatgtggtcaacctgaaggcgggcgaggtggccaggcttctggttcccttgagggaaaagggccggaccgtttttcactgccacatcgtggagcacgaggaccgggggatgatgggagtcctcgaggtgggttagtactagagaaaaaatcaaactggctcaccttcgggtgggcctttttgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z