LOCUS       BBa_K2037016            1638 bp    DNA     linear       22-Dec-2024
DEFINITION  Laccase for in vitro expression
ACCESSION   BBa_K2037016
VERSION     BBa_K2037016.1  
FEATURES             Location/Qualifiers
     promoter        1..18
                     /label=BBa_K2037000
     promoter        27..49
                     /label=T7 
     RBS             51..56
                     /label=RBS
     misc_feature    60..62
                     /label=ATG
     CDS             60..1559
                     /label=bhal
     misc_feature    1560..1577
                     /label=6 x HIS
     misc_feature    1578..1580
                     /label=TAA
     misc_feature    1581..1583
                     /label=TAA
     CDS             27..1583
                     /label=BBa_K863020
     stem_loop       1605..1624
                     /label=T7 TE
     misc_feature    1631..1631
                     /label=stop
     misc_feature    1628..1628
                     /label=C
     misc_feature    1601..1601
                     /label=C
     polyA_site      1592..1597
                     /label=''
     polyA_site      1625..1638
                     /label=polya
     misc_feature    1592..1638
                     /label=BBa_B0013
     terminator      1592..1638
                     /label=BBa_B0013
ORIGIN
        1 atttaggtga cactatagta ctagagtaat acgactcact atagggagag aggagaaaaa
       61 tgaaaaaatc atatggagtg atgggaggcg tccttgctgt cttcttcgca atagctggct
      121 gttcatccga cgatcattct gaaatggatc acggtaacaa tcagcaagat gaacaaaatg
      181 aagggacatt tgaagatggg aagaaagtgt tcaacctcga ggtaacagaa acacattgga
      241 tgtttaatga cgaggtgatg atggatgctt ggacttacaa tgggacgtta ccagggcaag
      301 aaatcagagt ccaagaggga gatgaagttg tcataaacgt gaaaaactcc ctcaatgtcc
      361 caactgccct tcatttacat gggtttcctg ttccaaatga aatggacgga gttccgggcg
      421 ttacgcaaaa tgcgattatg ccaggtgaag agtttacgta tgaatatcaa gcaaatgtac
      481 caggaactta ctggtatcat tcacaccaag acggggcgac tcaagtggat aggggtcttt
      541 acggtgtttt tattgtggaa ccagtggacc cagagtctta tgatacagat gtagctatcg
      601 ttatagacga gtttgcttct atgggaatgg gtatgggtat ggatcacagt accatggacc
      661 atggagatat ggaacacgac gggaacgacg acatgtctca ttcagaaatg atgaatgaga
      721 tgtatgacac gatggtaatt aacggaaagg ccgatccaca aatagaatcg atcaatgtag
      781 tagaaggaaa tagagtgaaa cttcgttttg tgaatgcagg gttgtttaca caagtggtat
      841 ctataccaga gcactccttt aaaatcacgc attacgacgg tcaacctgta aacgaaccgg
      901 atgttttaca ggatacggcc ttccgtatcg caccagccga acgctacgat cttgaaatta
      961 taatggataa ccctggagcg tggggcattc aagtatttgc tgaagaaaat caagagcgac
     1021 tacaagcgtt aattcctctt gtatatgaag agtatgaaga cgaagaatta caaacagtcg
     1081 attccatttc ctcattcttt gatttaacca cgtatgggat accgaaagaa ttggacatag
     1141 gagacattac aaaagaatat gacatgctcc ttggatcaga tgatggtggt gacaccttta
     1201 cgattaacgg aaagaaattt ccaaaccatg aaatatacga tgtcgaagaa ggggatatgg
     1261 taaagttcac gatcatcaac gatacagatt tcgaccatcc gatgcatttg catggggatt
     1321 tcttccacgt catttccaag ggcgggaagg ctattcaagg ctctccggtt ttaaaagata
     1381 cgctcaatgt cagacctgga gaaacgtatg agattgtatt tgaagcgaaa aatcctggaa
     1441 actggatgtt tcactgtcat gagtttcatc atgcaagcgg tggaatggta gcagaaattc
     1501 attatgaagg gtttgaactt ccatttacgc cagatcctaa tattccaaat atgcctgagc
     1561 atcaccatca ccatcactaa taatactaga gaaaaaatca aactggctca ccttcgggtg
     1621 ggcctttttg cgtttata
//