BBa_K2037016 1 BBa_K2037016 Laccase for in vitro expression 2016-10-13T11:00:00Z 2016-10-14T03:22:21Z Bacillus halodurans Sp6 promoter combined with bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag and a T7 terminator false false _2504_ 26994 26994 9 false None false Vaughn Murray Barendsen component2509596 1 BBa_K2037000 component2509604 1 BBa_K863020 component2509606 1 BBa_B0013 annotation2509596 1 BBa_K2037000 range2509596 1 1 18 annotation2509604 1 BBa_K863020 range2509604 1 27 1583 annotation2509606 1 BBa_B0013 range2509606 1 1592 1638 BBa_B0013 1 BBa_B0013 TE from coliphage T7 (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream.<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7021 1 BBa_B0013 range7021 1 1 47 annotation1695 1 C range1695 1 37 37 annotation1697 1 '' range1697 1 1 6 annotation1691 1 T7 TE range1691 1 14 33 annotation1696 1 C range1696 1 10 10 annotation1692 1 stop range1692 1 40 40 annotation1698 1 polya range1698 1 34 47 BBa_K2037000 1 BBa_K2037000 SP6 Promoter 2016-10-11T11:00:00Z 2016-10-12T03:41:10Z SP6 bacteriophage. SP6 phage promoter that is recognised by SP6 RNA polymerase. This part can be used to assemble a device which can be expressed in vitro, by making use of the bacteriophage SP6 RNA Polymerase. false false _2504_ 26774 26774 9 false None false Ricu Claassens BBa_K863020 1 BBa_K863020 bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag 2012-09-18T11:00:00Z 2015-05-08T01:13:36Z Bacillus halodurans C-125 Released HQ 2013 bhal laccase from Bacillus halodurans with T7 and HIS false false _1123_ 0 12844 9 In stock false test false Isabel Huber annotation2202251 1 T7 range2202251 1 1 23 annotation2202257 1 TAA range2202257 1 1555 1557 annotation2202256 1 TAA range2202256 1 1552 1554 annotation2202255 1 6 x HIS range2202255 1 1534 1551 annotation2202254 1 bhal range2202254 1 34 1533 annotation2202252 1 RBS range2202252 1 25 30 annotation2202253 1 ATG range2202253 1 34 36 BBa_B0013_sequence 1 aaaaaatcaaactggctcaccttcgggtgggcctttttgcgtttata BBa_K2037016_sequence 1 atttaggtgacactatagtactagagtaatacgactcactatagggagagaggagaaaaatgaaaaaatcatatggagtgatgggaggcgtccttgctgtcttcttcgcaatagctggctgttcatccgacgatcattctgaaatggatcacggtaacaatcagcaagatgaacaaaatgaagggacatttgaagatgggaagaaagtgttcaacctcgaggtaacagaaacacattggatgtttaatgacgaggtgatgatggatgcttggacttacaatgggacgttaccagggcaagaaatcagagtccaagagggagatgaagttgtcataaacgtgaaaaactccctcaatgtcccaactgcccttcatttacatgggtttcctgttccaaatgaaatggacggagttccgggcgttacgcaaaatgcgattatgccaggtgaagagtttacgtatgaatatcaagcaaatgtaccaggaacttactggtatcattcacaccaagacggggcgactcaagtggataggggtctttacggtgtttttattgtggaaccagtggacccagagtcttatgatacagatgtagctatcgttatagacgagtttgcttctatgggaatgggtatgggtatggatcacagtaccatggaccatggagatatggaacacgacgggaacgacgacatgtctcattcagaaatgatgaatgagatgtatgacacgatggtaattaacggaaaggccgatccacaaatagaatcgatcaatgtagtagaaggaaatagagtgaaacttcgttttgtgaatgcagggttgtttacacaagtggtatctataccagagcactcctttaaaatcacgcattacgacggtcaacctgtaaacgaaccggatgttttacaggatacggccttccgtatcgcaccagccgaacgctacgatcttgaaattataatggataaccctggagcgtggggcattcaagtatttgctgaagaaaatcaagagcgactacaagcgttaattcctcttgtatatgaagagtatgaagacgaagaattacaaacagtcgattccatttcctcattctttgatttaaccacgtatgggataccgaaagaattggacataggagacattacaaaagaatatgacatgctccttggatcagatgatggtggtgacacctttacgattaacggaaagaaatttccaaaccatgaaatatacgatgtcgaagaaggggatatggtaaagttcacgatcatcaacgatacagatttcgaccatccgatgcatttgcatggggatttcttccacgtcatttccaagggcgggaaggctattcaaggctctccggttttaaaagatacgctcaatgtcagacctggagaaacgtatgagattgtatttgaagcgaaaaatcctggaaactggatgtttcactgtcatgagtttcatcatgcaagcggtggaatggtagcagaaattcattatgaagggtttgaacttccatttacgccagatcctaatattccaaatatgcctgagcatcaccatcaccatcactaataatactagagaaaaaatcaaactggctcaccttcgggtgggcctttttgcgtttata BBa_K863020_sequence 1 taatacgactcactatagggagagaggagaaaaatgaaaaaatcatatggagtgatgggaggcgtccttgctgtcttcttcgcaatagctggctgttcatccgacgatcattctgaaatggatcacggtaacaatcagcaagatgaacaaaatgaagggacatttgaagatgggaagaaagtgttcaacctcgaggtaacagaaacacattggatgtttaatgacgaggtgatgatggatgcttggacttacaatgggacgttaccagggcaagaaatcagagtccaagagggagatgaagttgtcataaacgtgaaaaactccctcaatgtcccaactgcccttcatttacatgggtttcctgttccaaatgaaatggacggagttccgggcgttacgcaaaatgcgattatgccaggtgaagagtttacgtatgaatatcaagcaaatgtaccaggaacttactggtatcattcacaccaagacggggcgactcaagtggataggggtctttacggtgtttttattgtggaaccagtggacccagagtcttatgatacagatgtagctatcgttatagacgagtttgcttctatgggaatgggtatgggtatggatcacagtaccatggaccatggagatatggaacacgacgggaacgacgacatgtctcattcagaaatgatgaatgagatgtatgacacgatggtaattaacggaaaggccgatccacaaatagaatcgatcaatgtagtagaaggaaatagagtgaaacttcgttttgtgaatgcagggttgtttacacaagtggtatctataccagagcactcctttaaaatcacgcattacgacggtcaacctgtaaacgaaccggatgttttacaggatacggccttccgtatcgcaccagccgaacgctacgatcttgaaattataatggataaccctggagcgtggggcattcaagtatttgctgaagaaaatcaagagcgactacaagcgttaattcctcttgtatatgaagagtatgaagacgaagaattacaaacagtcgattccatttcctcattctttgatttaaccacgtatgggataccgaaagaattggacataggagacattacaaaagaatatgacatgctccttggatcagatgatggtggtgacacctttacgattaacggaaagaaatttccaaaccatgaaatatacgatgtcgaagaaggggatatggtaaagttcacgatcatcaacgatacagatttcgaccatccgatgcatttgcatggggatttcttccacgtcatttccaagggcgggaaggctattcaaggctctccggttttaaaagatacgctcaatgtcagacctggagaaacgtatgagattgtatttgaagcgaaaaatcctggaaactggatgtttcactgtcatgagtttcatcatgcaagcggtggaatggtagcagaaattcattatgaagggtttgaacttccatttacgccagatcctaatattccaaatatgcctgagcatcaccatcaccatcactaataa BBa_K2037000_sequence 1 atttaggtgacactatag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z