BBa_K863021 1 BBa_K863021 bhal laccase from Bacillus halodurans 2012-09-22T11:00:00Z 2015-05-08T01:13:36Z Bacillus halodurans C-125 Released HQ 2013 bhal laccase from Bacillus halodurans false false _1123_ 0 12844 9 In stock false test false Isabel Huber annotation2213230 1 ATG range2213230 1 1 3 annotation2213229 1 CDS range2213229 1 1 1503 annotation2213231 1 TAA range2213231 1 1501 1503 BBa_K2037000 1 BBa_K2037000 SP6 Promoter 2016-10-11T11:00:00Z 2016-10-12T03:41:10Z SP6 bacteriophage. SP6 phage promoter that is recognised by SP6 RNA polymerase. This part can be used to assemble a device which can be expressed in vitro, by making use of the bacteriophage SP6 RNA Polymerase. false false _2504_ 26774 26774 9 false None false Ricu Claassens BBa_B0013 1 BBa_B0013 TE from coliphage T7 (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream.<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7021 1 BBa_B0013 range7021 1 1 47 annotation1691 1 T7 TE range1691 1 14 33 annotation1697 1 '' range1697 1 1 6 annotation1695 1 C range1695 1 37 37 annotation1696 1 C range1696 1 10 10 annotation1692 1 stop range1692 1 40 40 annotation1698 1 polya range1698 1 34 47 BBa_K2037017 1 BBa_K2037017 Laccase for in vitro expression 2016-10-13T11:00:00Z 2016-10-14T03:26:26Z Bacillus halodurans Sp6 promoter joined to bhal laccase from Bacillus halodurans joined to a T7 terminator false false _2504_ 26994 26994 9 false None false Vaughn Murray Barendsen component2509704 1 BBa_B0013 component2509702 1 BBa_K863021 component2509698 1 BBa_K2037000 annotation2509698 1 BBa_K2037000 range2509698 1 1 18 annotation2509702 1 BBa_K863021 range2509702 1 25 1527 annotation2509704 1 BBa_B0013 range2509704 1 1536 1582 BBa_K863021_sequence 1 atgaaaaaatcatatggagtgatgggaggcgtccttgctgtcttcttcgcaatagctggctgttcatccgacgatcattctgaaatggatcacggtaacaatcagcaagatgaacaaaatgaagggacatttgaagatgggaagaaagtgttcaacctcgaggtaacagaaacacattggatgtttaatgacgaggtgatgatggatgcttggacttacaatgggacgttaccagggcaagaaatcagagtccaagagggagatgaagttgtcataaacgtgaaaaactccctcaatgtcccaactgcccttcatttacatgggtttcctgttccaaatgaaatggacggagttccgggcgttacgcaaaatgcgattatgccaggtgaagagtttacgtatgaatatcaagcaaatgtaccaggaacttactggtatcattcacaccaagacggggcgactcaagtggataggggtctttacggtgtttttattgtggaaccagtggacccagagtcttatgatacagatgtagctatcgttatagacgagtttgcttctatgggaatgggtatgggtatggatcacagtaccatggaccatggagatatggaacacgacgggaacgacgacatgtctcattcagaaatgatgaatgagatgtatgacacgatggtaattaacggaaaggccgatccacaaatagaatcgatcaatgtagtagaaggaaatagagtgaaacttcgttttgtgaatgcagggttgtttacacaagtggtatctataccagagcactcctttaaaatcacgcattacgacggtcaacctgtaaacgaaccggatgttttacaggatacggccttccgtatcgcaccagccgaacgctacgatcttgaaattataatggataaccctggagcgtggggcattcaagtatttgctgaagaaaatcaagagcgactacaagcgttaattcctcttgtatatgaagagtatgaagacgaagaattacaaacagtcgattccatttcctcattctttgatttaaccacgtatgggataccgaaagaattggacataggagacattacaaaagaatatgacatgctccttggatcagatgatggtggtgacacctttacgattaacggaaagaaatttccaaaccatgaaatatacgatgtcgaagaaggggatatggtaaagttcacgatcatcaacgatacagatttcgaccatccgatgcatttgcatggggatttcttccacgtcatttccaagggcgggaaggctattcaaggctctccggttttaaaagatacgctcaatgtcagacctggagaaacgtatgagattgtatttgaagcgaaaaatcctggaaactggatgtttcactgtcatgagtttcatcatgcaagcggtggaatggtagcagaaattcattatgaagggtttgaacttccatttacgccagatcctaatattccaaatatgcctgagtaa BBa_K2037017_sequence 1 atttaggtgacactatagtactagatgaaaaaatcatatggagtgatgggaggcgtccttgctgtcttcttcgcaatagctggctgttcatccgacgatcattctgaaatggatcacggtaacaatcagcaagatgaacaaaatgaagggacatttgaagatgggaagaaagtgttcaacctcgaggtaacagaaacacattggatgtttaatgacgaggtgatgatggatgcttggacttacaatgggacgttaccagggcaagaaatcagagtccaagagggagatgaagttgtcataaacgtgaaaaactccctcaatgtcccaactgcccttcatttacatgggtttcctgttccaaatgaaatggacggagttccgggcgttacgcaaaatgcgattatgccaggtgaagagtttacgtatgaatatcaagcaaatgtaccaggaacttactggtatcattcacaccaagacggggcgactcaagtggataggggtctttacggtgtttttattgtggaaccagtggacccagagtcttatgatacagatgtagctatcgttatagacgagtttgcttctatgggaatgggtatgggtatggatcacagtaccatggaccatggagatatggaacacgacgggaacgacgacatgtctcattcagaaatgatgaatgagatgtatgacacgatggtaattaacggaaaggccgatccacaaatagaatcgatcaatgtagtagaaggaaatagagtgaaacttcgttttgtgaatgcagggttgtttacacaagtggtatctataccagagcactcctttaaaatcacgcattacgacggtcaacctgtaaacgaaccggatgttttacaggatacggccttccgtatcgcaccagccgaacgctacgatcttgaaattataatggataaccctggagcgtggggcattcaagtatttgctgaagaaaatcaagagcgactacaagcgttaattcctcttgtatatgaagagtatgaagacgaagaattacaaacagtcgattccatttcctcattctttgatttaaccacgtatgggataccgaaagaattggacataggagacattacaaaagaatatgacatgctccttggatcagatgatggtggtgacacctttacgattaacggaaagaaatttccaaaccatgaaatatacgatgtcgaagaaggggatatggtaaagttcacgatcatcaacgatacagatttcgaccatccgatgcatttgcatggggatttcttccacgtcatttccaagggcgggaaggctattcaaggctctccggttttaaaagatacgctcaatgtcagacctggagaaacgtatgagattgtatttgaagcgaaaaatcctggaaactggatgtttcactgtcatgagtttcatcatgcaagcggtggaatggtagcagaaattcattatgaagggtttgaacttccatttacgccagatcctaatattccaaatatgcctgagtaatactagagaaaaaatcaaactggctcaccttcgggtgggcctttttgcgtttata BBa_B0013_sequence 1 aaaaaatcaaactggctcaccttcgggtgggcctttttgcgtttata BBa_K2037000_sequence 1 atttaggtgacactatag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z