BBa_K2039000
1
BBa_K2039000
Part expressing the dimerization protein FKBP and GFP10 (subunit of tripartite GFP)
2016-10-11T11:00:00Z
2016-10-14T12:58:32Z
Synthetic
This part is the complementary part of Bba_K2039001.
The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been optimized in order to be expressed in bacteria: mutations (T2098L, K2095P, W2101F) for dimerization with rapalog and optimization by Jcat plateforme.
FKBP protein is linked to GFP 10.
The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).
false
false
_2506_
31112
31202
9
Not in stock
false
No issues
false
Maxence Pfeiffer
component2506106
1
BBa_J23119
component2506117
1
BBa_B0015
component2506108
1
BBa_B0030
component2506110
1
BBa_K2039003
annotation2506106
1
BBa_J23119
range2506106
1
1
35
annotation2506117
1
BBa_B0015
range2506117
1
603
731
annotation2506110
1
BBa_K2039003
range2506110
1
67
594
annotation2506108
1
BBa_B0030
range2506108
1
44
58
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K2039003
1
BBa_K2039003
Coding sequences of proteins FKBP and GFP10 (subunit of tripartite GFP)
2016-10-13T11:00:00Z
2016-10-14T12:57:36Z
Synthetic.
This part is the complementary part of Bba_K2039004.
The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the medium. It is particularly useful for protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria.
FKBP protein is linked to GFP 10.
The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).
false
false
_2506_
31112
31202
9
Not in stock
false
No specific considerations
false
Maxence Pfeiffer
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K2039000_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtactagatgatgggtgttcaggttgaaaccatctctccgggtgacggtcgtaccttcccgaaacgtggtcagacctgcgttgttcactacaccggtatgctggaagacggtaaaaaattcgactcttctcgtgaccgtaacaaaccgttcaaattcaccctgggtaaacaggaagttatccgtggttgggaagaaggtgttgctcagatgtctgttggtcagcgtgctaaactgatcatctcttctgactacgcttacggtgctaccggtcacccgggtatcatcccgccgcacgctaccctggttttcgacgttgaactgctgaaactggaagatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcatggatctgccggatgatcattatctgagcacccagaccattctgagcaaagatctgaactaataacgctgatagtgctagtgtagatcgctactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_K2039003_sequence
1
tactagatgatgggtgttcaggttgaaaccatctctccgggtgacggtcgtaccttcccgaaacgtggtcagacctgcgttgttcactacaccggtatgctggaagacggtaaaaaattcgactcttctcgtgaccgtaacaaaccgttcaaattcaccctgggtaaacaggaagttatccgtggttgggaagaaggtgttgctcagatgtctgttggtcagcgtgctaaactgatcatctcttctgactacgcttacggtgctaccggtcacccgggtatcatcccgccgcacgctaccctggttttcgacgttgaactgctgaaactggaagatgtaggaggtggaggtagtgaaggaggaggtagcggtggtccgggaagcgcaggcgaaggcagcgcggtaggaggttctgctggaggtggcagcatggatctgccggatgatcattatctgagcacccagaccattctgagcaaagatctgaactaataacgctgatagtgctagtgtagatcgctactagag
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z